Beer, Evaluation of Final Product and Filtration Efficiency
The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method. A suitable electrolyte solution is required to perform the analysis.The sample is prepared by dissolving a certain volume of beer in the electrolyte and then analyzed using a Beckman Coulter Multisizer 3 to determine the size distribution and concentration for the particles present in the beer. The results are reported as number of particles per milliliter for the desired size range.The use of the Multisizer 3 provides a fast, easy, accurate and automatic method to determine the particle content in beer. The use of this instrument also provides reliable results not dependent on the operator’s judgment making it possible to compare data from different work shifts and/or breweries.
SIGNIFICANCE
The determination of particle concentration in beers is important for evaluating and/or correcting several steps during the brewing process and finishing of the product.- Evaluation of the Final Product. Each kind of beer has its own characteristics and distinctive flavor; these properties will be influenced to some extent by the content and size distribution of particles present in the final product.The stability and therefore the shelf life of beer are also affected by its particle content.
- Evaluation of Chill Haze Effect. This is the most common, and in some sense, the most important type of beer hazesince it is relevant to many beer types. As the name suggests, this haze appears when the beer is suitably chilled; the haze disappears upon warming. The temperatures at which the haze appears and disappears depend on the physical stability of the beer.The more stable the beer, the closer to 0 °C before chill haze occurs.The haze involves complexes of highmolecularweight proteins and polyphenols (tannins). These compounds form weak, temperature sensitive
hydrogen bonds that are broken as the beer’s temperature increases, allowing the resulting compounds to form a complex with water molecules and go into solution. - Filtration Efficiency. Brewers have been using some type of filtration for centuries. If properly used, it can serve as an effective nonadditive tool in beer clarification. Filtration is used in conjunction with fining agents to render beer brilliantly clear and stable with respect to temperature changes.
In this paper we will refer to the evaluation of the final product and filtration efficiency.
EVALUATION OF FINAL PRODUCT
INSTRUMENT SET UP AND CALIBRATION
A 50 µm aperture tube is used for the evaluation of the final product.The linear dynamic range for any aperture is 2% to 60% of its size, i.e. a 50 µm aperture tube will be capable of analyze the particle concentration and size distribution from 1 µm to 30 µm. Set up and calibrate the instrument according to the Multisizer 3 Operator’s Manual. For determining particle concentration the control mode for the instrument must be Volumetric Mode, select 500 µL.
PROCEDURE
- Running a Background
Entering background information in the Multisizer 3 Software. By entering the background information, the software will be able to calculate the concentration of particles in the Isoton.
- Sample Volume: 20 mL
- Electrolyte Volume: 0
- Analytical Volume 500µL
- Place 20 mL of Isoton® II in an Accuvette® II.
- Place into the analyzer the Accuvette® II containing the Isoton, flush the aperture tube before the run.
- On the Multisizer software set the background. The background will be automatically subtracted from all subsequent
- Analyzing the Sample
- Entering sample information in the Multisizer 3 Software.
Enter the required sample information in the software: analytical volume electrolyte (Isoton) volume and volume of beer used for the analysis. By entering the sample information, the software will be able to calculate the concentration of particles in the beer.
- Sample Volume: Enter the amount of sample to be use in the analysis
- Electrolyte Volume: Enter the amount of Isoton to be use in the analysis
- Analytical Volume 500 µL
- Sample preparation
After removing gas from the beer, measure exactly 15 mL of Isoton® II into a 20 mL Accuvette® II. Pipette 5.0 mL of beer into the Isoton, these quantities may be different according to the kind of beer, for example for Wheat Ales a smaller amount of sample and more Isoton will be needed. Cap the Accuvette and stir gently to dissolve thoroughly without creating bubbles. Prepare each sample at the moment it will be analyzed. - Place into the analyzer the Accuvette® II containing the sample, flush the aperture tube before the analysis.
- After each run rinse the aperture and electrode before proceeding to the next sample.
- Entering sample information in the Multisizer 3 Software.
- Reporting the results
Results are reported as the total number of particles per mL from 1 to 30 µm and /or particles per mL larger than 1, 2, 3, 4, 5, 10, 15 and 20 µm.
Particles / mL Larger than | ||||||||
1 µm | 2 µm | 3 µm | 4 µm | 5 µm | 10 µm | 15 µm | 20 µm | |
BECK'S | 10.213 | 2.493 | 1.012 | 538 | 281 | 88 | 51 | 7 |
BUDWEISER | 16.950 | 1.776 | 697 | 426 | 341 | 183 | 110 | 44 |
COORS LIGHT | 11.731 | 1.702 | 586 | 273 | 154 | 38 | 16 | 0 |
CORONA EXTRA | 3.601 | 751 | 377 | 231 | 147 | 59 | 29 | 11 |
FULLER'S LONDON PRIDE | 744.862 | 107.715 | 35.884 | 16.347 | 8.423 | 693 | 118 | 24 |
GRANT'S IPA | 330.673 | 58.915 | 15.908 | 5.800 | 2.655 | 205 | 48 | 9 |
HEINEKEN | 81.292 | 13.888 | 5.302 | 2.968 | 2.071 | 877 | 354 | 76 |
MILLER LITE | 3.144 | 747 | 378 | 298 | 217 | 76 | 45 | 14 |
MILLER MDG | 12.638 | 1.845 | 456 | 181 | 110 | 22 | 4 | 0 |
PRESIDENTE | 29.508 | 5.801 | 2.332 | 1.177 | 615 | 124 | 62 | 23 |
SAMUEL ADAMS | 352.452 | 80.701 | 27.613 | 12.089 | 6.204 | 700 | 133 | 32 |
SAM ADAMS WINTER LAGER | 87.196 | 12.763 | 4.501 | 2.048 | 961 | 69 | 7 | 0 |
SINGHA | 99.980 | 24.166 | 9.879 | 4.857 | 2.678 | 255 | 37 | 12 |
THE KNIGHT'S ALE (WHITE ALE) | 15.36 x 106 | 1.545 x 106 | 1.368 x 106 | 1.278 x 106 | 661.568 | 1.654 | 306 | 79 |
Particles/ml 1-30 µm) | Particles/ml 1-30 µm) | ||
Beck’s (Germany) | 10,213 | Miller Lite (USA) | 3,144 |
Budweiser (USA) | 16,950 | Miller MGD | 12,638 |
Coors Light (USA) | 11,731 | Presidente (Dominican Republic) | 29,508 |
Corona Extra (Mexico) | 3,601 | Samuel Adams (USA) | 352,452 |
Fuller’s London Pride (UK) | 744,862 | Sam.Adams Winter Lager (USA) | 87,196 |
Grant’s IPA (USA) | 330,673 | Singha (Thailand) | 99,980 |
Heineken (Holland) | 81,292 | The Knight’s Ale (Belgium White Ale) | 15.36 x 106 |
The above data does not represent by any means a comparison for different brands of beer.The samples were randomly selected from brands and kinds of beers available at the market, therefore they have different characteristics and they have been manufactured at different dates and stored under diverse conditions and length of time.The only purpose of this table and following graphs is to show how the results are reported.
DETERMINATION OF SIZE AND CONCENTRATION OF PARTICLES IN BEER FINAL PRODUCT
Comparison Graph for Different Kinds of Beer
Belgian Wheat Ale
FILTRATION EFFICIENCY
Set up the instrument and follow the same procedure described for the final product steps 1 through 2.4. Perform the analysis for beer getting into the filter and coming out from the filter.
REPORTING THE RESULTS
The efficiency of the filter is determined by comparing the results before and after filtration.The amount of particles removed as a percentage of the particles present before the filtration gives the percentage of efficiency.
The filtration process may also be monitored for specific size ranges.The total number of particles removed not always provide a complete picture of a filtration deficiency, sometimes it is necessary to target certain size range for adjusting the filtration process.
Particle Diameter (µm) | Filter IN Number per mL larger than |
Filter OUT Number per mL larger than |
Efficiency (%) |
1 | 35.296 | 1.875 | 94.68 |
2 | 5.427 | 744 | 86.29 |
3 | 1.560 | 327 | 79.02 |
4 | 498 | 135 | 72.95 |
5 | 244 | 77 | 68.30 |
10 | 56 | 19 | 66.66 |
15 | 22 | 8 | 63.61 |
20 | 4 | 0 | 100 |
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- Flow Cytometry Testing in Hospital Laboratories
- Fundamentals of Ultracentrifugal Virus Purification
- Tumor Suppressor Gene p53 research and DNA Cleanup Process
- Fundamentals of Ultracentrifugal Virus Purification
- Dr Yabui UCF Lecture
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Posters
- Applications of Ultracentrifugation in Purification and Characterization of Biomolecules
- Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
- ABRF 2019: Automated Genomic DNA Extraction from Large Volume Whole Blood
- Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation
- Automating Cell Line Development for Biologics
- Cell-Line Engeneering
- Characterizing the Light-Scatter Sensitivity of the CytoFLEX Flow Cytometer
- AACR 2019: Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample
- Mastering Cell Counting
- Preparing a CytoFLEX for Nanoscale Flow Cytometry
- A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis
- ABRF 2019: Simultaneous DNA and RNA Extraction from Formalin-Fixed Paraffin Embedded (FFPE) Tissue
- Quantification of AAV Capsid Loading Fractions: A Comparative Study
- Using Standardized Dry Antibody Panels for Flow Cytometry in Response to SARS-CoV2 Infection
- Product Instructions
- Experimental Protocols
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Whitepapers
- Centrifugation is a complete workflow solution for protein purification and protein aggregation quantification
- AUC Insights - Analysis of Protein-Protein-Interactions by Analytical Ultracentrifugation
- A General Guide to Lipid Nanoparticles
- Analytical Ultracentrifugation: A Versatile and Valuable Technique for Macromolecular Characterization
- Addressing issues in purification and QC of Viral Vectors
- GMP Cleanrooms Classification and Routine Environmental Monitoring
- Purification of Biomolecules by DGUC
- AUC Insights - Assessing the quality of adeno-associated virus gene therapy vectors by sedimentation velocity analysis
- AUC Insights - Sample concentration in the Analytical Ultracentrifuge AUC and the relevance of AUC data for the mass of complexes, aggregation content and association constants
- Analyzing Biological Systems with Flow Cytometry
- Changes to USP <1788> Subvisible Particulate Matter
- Changes to USP <643> Total Organic Carbon
- Characterization of RNAdvance Viral XP RNA Extraction Kit using AccuPlex™ SARS–CoV–2 Reference Material Kit
- CytoFLEX Platform Gain Independent Compensation Enables New Workflows
- CytoFLEX Platform Flow Cytometers with IR Laser Configurations: Considerations for Red Emitting Dyes
- Evaluation of the Analytical Performance of the AQUIOS CL Flow Cytometer in a Multi-Center Study
- Simultaneous Isolation and Parallel Analysis of gDNA and total RNA for Gene Therapy
- Hydraulic Particle Counter Sample Preparation
- Inactivation of COVID–19 Disease Virus SARS–CoV–2 with Beckman Coulter Viral RNA Extraction Lysis Buffers
- Tips for Cell Sorting
- Liquid Biopsy Cancer Biomarkers – Current Status, Future Directions
- MET ONE 3400+ IT Implementation Guide
- Reproducibility in Flow Cytometry
- SuperNova v428: New Bright Polymer Dye for Flow Cytometry
- SuperNova v428: New Bright Polymer Dye for Flow Cytometry
- Japan Document
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Application Notes