Posters

Each year, Beckman Coulter scientists present posters on diverse topics at conferences, meetings and symposia around the world. 

For your convenience, we’ve collected all these posters in one place. 

What’s your area of interest?

Title Description
Fluorochrome Guide MoFlo XDP and MoFlo Astrios
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Standard MoFlo XDP Fluorochromes
CytoFLEX System Performance Evaluation
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Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus , John Nolan, Kathy Ragheb, Jennifer Sturgis, J Paul Robinson, Zhao, Jing, Domenic Fenoglio, Yijun, Huang, Paul Scibelli | Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center, Sun Yat-Sen University, School of Medicine, Guangzhou, China
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents
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Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung - Beckman Coulter, Miami FL
FoxP3+ Tregs Cells Sensitivity to Low Doses of IL-2 Can be Rapidly and Easily Monitored by p-STAT-5 Detection in Ex-Vivo Whole Blood Samples
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Authors: Fabrice Malergue, Valarie Mallet, Caroline Scifo, Tewfik Miloud, Felix A. Montero-Julian | Beckman Coulter Life Sciences, Inc. ; Global Assay and Applications Development, Marseille, France
Designing Multi-Color Panels for T/Treg Populations using FITMaN Guidelines: a Case Study
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Authors: James W Tung, Jonel Lawson, Carlos A Garcia, Jeffrey R Cobb, Yetty Irwan, William Godfrey, Michael Reed, Enrique Rabellino | Beckman Coulter Inc, Cellular Analysis Business Group, Scientific Affairs, Miami FL
Twenty-seven Markers on a 3-laser Flow Cytometer
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This poster was presented at Cyto in 2018 and demonstrates a technique to maximize the usage of channels on a flow cytometer. The method relies on stacking biomarkers that are not expressed on the same cell and a gating technique to interrogate the populations of interest.
Demonstration of the Analytical Performance of the Navios Flow Cytometer in a Multi-Center Study
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Authors: Diana Careaga, Robert Magari, Karen Lo, Michael Keeney, Janice Popma, Joanne Luider, Aito Ueno, Gerard Lozanski, Bruce Briggs, Elena Afonina, Enrique Rabellino and Liliana Tejidor
SWOFF - The Unrecognized Sibling of FMO
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Author: Michael Kapinsky - Beckman Coulter Intl.
MoFlo™ Astrios* Enhanced Forward Scatter+: 0.2 μm to 30 μm Dynamic FSC Detection
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Authors: Dr. Carley D. Ross, Mike Morrell, Robin Morris, Blake McCarty, Alan Dean Ph.D. and Nicole Lewis - Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
Comparison between Mutation Profiles of Paired Whole Blood and cfDNA Samples
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Review our poster we presented at ASHG 2019 where we looked at the data associated with the comparison between mutation profiles of paired whole blood and cfDNA samples
ASHG 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
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Review our poster we presented at ASHG 2019 where we looked at the data associated with correlation between mutations found In FFPE tumor tissue and paired cfDNA samples
AMP 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
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In this poster, presented at AMP 2019, we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis
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In this poster, we will demonstrate the VSSC-based size resolution and fluorescence sensitivity of our prototype using a variety of NIST-traceable size standards and fluorescent nanoparticles
An Analytical Revolution: Introducing the Next Generation Optima AUC
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Author: Chad Schwartz, Ph.D. | Beckman Coulter Life Sciences
Immunophenotyping reference table - For haematopoietic malignancies
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Poster - Immunophenotyping reference table - For haematopoietic malignancies
Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation
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Here we describe the automation of the MCI Advantage Cancer Panel at Miami Cancer Institute, a 170 gene panel assay based upon the Illumina TruSight® Tumor 170 Panel, on the Beckman Coulter Biomek i5 Span-8 NGS Workstation
Roche GS FLX Titanium* Rapid Library Preparation Kit Automation Using Biomek® FXp Laboratory Automation Workstation
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Authors: Ruth Zhang Ph.D. , Zach Smith , Laura Pajak Ph.D. , Keithanne Mockaitis, Ph.D. , John Colbourne Ph.D. , and Alisa Jackson - Beckman Coulter, Inc., Indianapolis, Indiana and The Center for Genomics and Bioinformatics, Indiana University, Bloomington, Indiana
Automation of a Cell-Based Screening Assay for Intracellular Apoptosis Activity using the Biomek Assay Workstation
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Authors: Yu Suen, Ph.D; Keith Roby; Javorka Gunic ; and Graham Threadgill, Ph.D - Biomedical Research Division, Beckman Coulter, Inc.
Characterizing the Light-Scatter Sensitivity of the CytoFLEX Flow Cytometer
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The CytoFLEX is so sensitive that the SSC detector actually has an attenuation filter to reduce 95% of the scatter signal, adjusting it to a range useful for cells
GenFind V3: Automated genomic DNA extraction from large volume whole blood
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In this poster we present an automated, high-quality, research-ready DNA extraction method from large volume blood. GenFind V3 DNA isolation kit automated on the KingFisher Duo Prime system provides scalable and consistent system for gDNA extraction from 2 mL of whole blood.
Accelerate Biomarker Discovery and Improve Results Using Automation
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Author: Zach Smith, MS | Published 2017
Automated TruSeq RNA Sample Preparation from FFPE Tissue Specimens Utilizing the Biomek FXP Liquid Handler
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Authors: Michaela Bowden Ph.D., Ashley Rossi, Bee Na Lee Ph.D., Sarah Simons Svitlana Tyekucheva, Ed Stack Ph.D., Massimo Loda MD., | Dana Farber Cancer Institute and Brigham, Women’s Hospital and Beckman Coulter Life Sciences
Next Generation Sequencing High-throughput Solution for Illumina Fragment Library Sample Preparation
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Authors: Scott Verrow, Kevin Boynton, Mary Blair, Kelly Marshall, Ruth Zhang, Alisa Jackson | Beckman Coulter, Inc.
Enrichment and Characterization of Ferritin for Nanomaterial Applications
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Authors: Rodolfo GhirlandoƗ, Radina Mutskova¥, Chad SchwartzɈ | National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Honors College, Integrated Life Sciences, University of Maryland |Beckman Coulter, Inc., Indianapolis, IN, 46268
Automation of a Cell-Based Screening Assay for Intracellular Apoptosis Activity using the Biomek Assay Workstation
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Automation of the assay is achieved using the Biomek Assay Workstation with a Biomek NX integrated with a Cytomat* incubator and storage carousel for cell preparation, apoptosis induction and detection.
Cell Signaling Assay - Hardware Integration on Beckman Coulter Biomek
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Authors: Valentin Quesada | Kelechi Eluwa | Lidice Lopez | Vincent Shankey | Robert Auer | Sergei Gulnik
Using Violet Side Scatter to Detect and Resolve Nanoparticles on the CytoFLEX Flow Cytometer
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This poster provides detailed instructions for setting up the CytoFLEX flow cytometer Violet Side Scatter for nanoparticle detection. Experimental considerations for nanoscale flow cytometry and illustrated with data from the instrument.
A Flexible Solution for Automating Cell Staining Using the Biomek 4000 Workstation
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The automated Cell Staining Application on the Biomek 4000 Workstation can improve throughput and reproducibility of conventional staining of live and fixed adherent cells.
Moflo™ Astrios EQ Enhanced Forward Scatter: Simuultaneous Sorting of Micro- and Macro- Samples
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Authors: Alan Dean, PhD, Carly Ross, PhD, Kerry Weinberg, Blake McCarty, Robin Morris, and Mike Morrell- Beckman Coulter Fort Collins, CO
Automation of PCR Reaction Setup and AMPure XP Purification Using the Biomek 4000 Workstation
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Both polymerase chain reaction (PCR) reaction setup and AMPure XP PCR reagent-mediated purification were automated on the Biomek 4000 Laboratory Automation Workstation.
Automated NGS Sample Prep with Integrated Data Management and Reporting Tools
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Authors: Zach Smith, M.S. | John Snider, M.S. | David Horvath, M.S.
Measurement of ZAP- 70 Expression in CLL using an Optimized Flow Cytometric Assay for ZAP-70 Protein Levels in Whole Blood Samples
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ZAP-70 protein plays a critical role in the initiation of T-cell signaling through the TCR and also regulates B cell differentiation
Enrichment and Characterization of Ferritin for Nanomaterial Applications
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Authors: Rodolfo Ghirlando | Radina Mutskova | Chad Schwartz
To the Standardization of Exosome Isolation and Characterization
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Authors: Chad Schwartz, Ph.D. | Zach Smith, M.S.
Automate Like a Pro: Fully Integrated System for ELISA and Cell Viability Absorbance Assays
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Authors: Michael P. Kowalski | Tara Jones-Roe
MoFlo AstriosEQ™ Forward Scatter: Cell Sorting of Nano and Large Phytoplankton Simultaneously with High Purity
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Authors: Carley D. Ross Ph.D., and Robin Morris Beckman Coulter Life Sciences, Flow Cytometry Business Center - Fort Collins, CO
Roche GS FLX Titanium Rapid Library Preparation Kit Automation Using Biomek® FXp Laboratory Automation Workstation
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This poster presents the automated Roche GS FLX Titanium “Rapid Library Preparation Kit” using Beckman Coulter’s Biomek FXP dual hbrid laboratory automation workstation.
Automated TruSeq RNA Sample Preparation from FFPE tissue specimens utilizing the Biomek FXP Liquid Handler
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Describes a TruSeq RNA library preparation for FFPE samples using the Biomek automation platform, capable of constructing 96 libraries in 7 hours.
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
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Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
Evaluation of the Analytical Performance of the Aquios CL Flow Cytometer in a Multicenter Study
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Authors: Diana Careaga, Robert Magari, Karen Lo, Michael Keeney, Ben Hedley, Dominika Benjamins, Dr. You-Wen Qian, Larry Hinson, Cristina Ceja, Alexandra Amador Casiana Fernandez-Bango, Rosa Colon, Oilda Rubio, Eileen Landrum, Justin Rohrbach, Robert Ortega, Ana Vieira, Dawn Holmes, Raquel Cabana, Pedro Diaz and Liliana Tejidor | Beckman Coulter Diagnostics, Inc., Clinical Research, Miami, Florida, London Health Sciences Centre, London, Ontario, Canada, The University of Texas Medical Branch, Galveston, Texas, University of Miami Miller School of Medicine, Miami, Florida
Comparison of Aquios Tetra and Navios Tetra System Performance
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Authors: Violetta Headley, Michael Keeney, Dominika Benjamins, Justin Rohrbach, Robert Ortega, Karen Lo, Liliana Tejidor and Elena Afonina | Clinical Application Development, Life Sciences, Beckman Coulter, Inc London Health Sciences Centre, London, Ontario, Canada Clinical Affairs, Diagnostics Beckman Coulter, Inc
Automated NGS Sample Prep with Integrated Data Management and Reporting Tools
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Authors: Zach Smith, M.S., John Snider, M.S., David Horvath, M.S.- Beckman Coulter Life Sciences | Published 2015
Efficient Factorial Optimization of Transfection Conditions Poster
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Automate the creation of 96 factorial combinations of different transfection reagents, concentrations, and cell numbers to improve transfection efficiency
To the Standardization of Exosome Isolation and Characterization
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Authors: Chad Schwartz, Ph.D., Zach Smith, M.S. | Beckman Coulter, Inc., Indianapolis, IN, 46268
Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
In this poster we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
Automate Like a Pro: Fully Integrated System for ELISA and Cell Viability Absorbance Assays
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Authors: Michael P. Kowalski, Tara Jones-Roe | Beckman Coulter Life Sciences, Indianapolis, IN
Automation and Miniaturization of 3D Tumor Models for Compound Screens
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Authors: Michael Kowalski, Kayla Hill, Vipat Raksakulthai, Kristin Prasauckas, Tara Jones-Roe - Beckman Coulter Life Sciences, Indianapolis, IN Molecular Devices, Sunnyvale, CA
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry
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Poster: By integrating additional components such as incubators and cell analyzers to the Biomek FXP Workstation, the entire workflow can be automated in a scheduled fashion that further minimizes user interventions and helps manage overlapping experiments
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation
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To understand the changes that occur during differentiation of murine embryonic stem cells (mESCs)
Fully-Automated Cellular Analysis by Flow Cytometry Poster
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The Biomek i7 Automated Workstation was used to automate the complete cellular workflow for induction and analysis of apoptosis in two cancer lines
A High-Throughput and Reproducible Workflow for MRM Analysis of Biological Samples
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Authors: Qin Fu, Michael P Kowalski, Xiaoqian Liu, Jie Zhu, Graham J Threadgill, Christie L. Hunter and Jennifer E. Van Eyk | Johns Hopkins Bayview Proteomics Center, Division of Cardiology, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA | Beckman Coulter Life Sciences, Indianapolis, IN, USA, Beckman Coulter, Inc., Brea, CA, USA, 4AB SCIEX, Foster City, CA, USA
ProteomeLabtm XL-A/XL-I Protein Characterization System
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Native condition protein characterization with Beckman Coulter.
Mastering Cell Counting
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Image-based systems use bright field or fluorescent microscopy to capture an image of the cells
Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: William L. Godfrey, Li Yang, Samantha Bauchiero, James W. Tung, Valerie Mallet, Lidice Lopez, Vincent Shankey, Emmanuel Gautherot | Beckman Coulter Inc, Miami, FL, USA; Marseille, FRANCE
Beyond Matching Dim Expression Densities with Bright Fluorochromes A Novel Practical Approach to Design Multicolor Applications
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Author: Michael Kapinsky, Beckman Coulter Life Science, Inc., Krefeld, Germany
An 8-color Panel for Detection of Human Blood Dendritic Cells by Flow Cytometry
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Author: Tewfik Miloud, Nathalie Dupas, Anders Kverneland, Mathias Streitz, Birgit Sawitzki and Felix A. Montero-Julian | Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France , 2Institute of Medical Immunology, Charité Universitätsmedizin, Berlin, Germany Institute of Medical Immunology, Charité Universitätsmedizin, Berlin, Germany
An Automated Cell Preparation Method for FlowCARE™ Pan-Leukocyte Gating (PLG) CD4 Assay in a 96-Well Plate Offers Increased Throughput and Ease of Use
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: Jin Zhang, Valentin Quesada, Juan De Castro, Jennifer Zawislak, Jorge Acevedo, Ed Jachimowicz, and Liliana Tejidor | Beckman Coulter, Inc., Cellular Analysis Business Group, Miami, FL
Drift Analysis Approach to Investigate the Effect of Specimen Age and Prepared Sample Age on the Stability of Lymphocyte Subsets
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Authors: Robert Magari, Jin Zhang, Samantha Bauchiero, Violetta Headley, Liliana Tejidor, and Elena Afonina | Clinical Applications Development, Life Sciences, Beckman Coulter, Inc. - Clinical Research, Diagnostics, Beckman Coulter, Inc.
Krome Orange™: A Novel Orange-Emitting Dye for Use in Multicolor Flow Cytometry Panels Using Violet Diode Laser Excitation
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Authors: Felix Montero, Ravinder Gupta, Hashem Akhavan-Tafti, Robert Eickholt, Mark Sandison, Rhonda Federspiel, Laura Nieto Gligorovsky, Franck Gaille, Jeffrey Cobb and Emmanuel Gautherot | Beckman Coulter, Inc., Miami, FL, USA; Detroit, MI, USA; Marseille, FRANCE
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-Occurring Regulatory T-Cells
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Authors: Carlos A. Garcia Santana M.D., Ph.D., James W. Tung Ph.D. and Sergei Gulnik Ph.D. | Life Science Research; Life Science Development, Beckman Coulter, Inc., Miami, FL., USA
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. | Life Science Automation, Beckman Coulter Inc, Indianapolis, IN
Enumeration of T, B and NK Subpopulations in Aged Whole Blood Samples using AQUIOS CL Instrument with Tetra Application
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Authors: Violetta Headley, Xiangyang Dong , Raquel Cabana , Veronica Trujillo , Michael Thomas, Robert Magari, Elena Afonina | Clinical Application Development, Life Sciences, Beckman Coulter, Inc Research, Life Sciences Beckman Coulter Inc. Clinical Affairs, Diagnostics, Beckman Coulter, Inc
Simultaneously DNA and RNA extraction from formalin-fixed paraffin embedded (FFPE) tissue
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
In this poster we present a streamlined NGS ready to use sample prep workflow for FFPE tissue using a product in current development, FormaPure XL Total* (Beckman Coulter Life Science). FormaPure XL Total uses SPRI (Solid Phase Reverse Immobilization) paramagnetic beads to isolate DNA and RNA simultaneously.
Resolving Dyes with Identical Emission Wavelengths on the CytoFLEX
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Compensation in flow cytometry is a special case of spectral deconvolution. In this poster we demonstrate that the CytoFLEX can deconvolute highly overlapping fluorophores using the compensation algorithm.
How do I preserve sensitivity?
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Poster - How to preserve sensitivity
Nano- and Microparticle Bead Mix for Use as a Sizing Standard in Flow Cytometry
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The CytoFLEX platform is distinguished for not only its fluorescence sensitivity but also its ability to detect in the nanoparticle range using Violet Side Scatter. This poster, presented at Cyto in 2016 describes a NIST-traceable bead mix for use in standardizing flow cytometry experiments.
Flow Cytometric Analysis of Multiple Signaling Pathways in LPS Stimulated Human Peripheral Blood Monocytes
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Authors: T Shankey,Sue Chow, and David Hedley | Advanced Technology Group, Beckman Coulter, Inc., Miami, FL, USA , Division of Applied Molecular Oncology, Princess Margaret Hospital, Univ of Toronto, Toronto, Ont., Canada
Preparing a CytoFLEX for Nanoscale Flow Cytometry
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In this poster, we will demonstrate how to properly setup and clean a CytoFLEX flow cytometer for NP analyses
Comparison between mutation profiles of paired whole blood and cfDNA samples
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Next-Gen Sequencing of cfDNA is coming into maturity as a non-invasive method to identify mutational profiles in many cancer types
Automating Cell Line Development for Biologics
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Cell line development is a highly-involved, multi-week workflow that involves the plating, screening, and expansion of single cell-derived clones to establish a protein-expression cell line
CytoFLEX Instrument Evaluation Using Biological Specimens
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Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus, John Nolan, Kathy Ragheb, Jennifer Sturgis, Paul Scibelli, J Paul Robinson, Domenic Fenoglio | Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center
Impact of Clean-Up Kits on DNA Sequencing Quality and Efficiency
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
The applications of DNA sequencing in biological research are growing. A significant driver of this growth is Next-Generation Sequencing (NGS), a modern DNA sequencing technology instrumental in achieving complete DNA sequences or genomes of humans, many animals, plants, and microbial species.


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