Fluorescent amplification for next generation sequencing (FA-NGS) library preparation: A Streamlined Workflow to Improve NGS Quality Control Testing
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation: A Streamlined Workflow to Improve NGS Quality Control Testing Abstract: This webinar will discuss a streamlined library preparation method for next-generation sequencing (NGS) that improves quality control testing capability while reducing the total number of steps compared to conventional multiplexed library workflows. NGS library preparation methods typically entail quality control testing at the end of the workflow, which can be laborious, particularly for high-throughput applications. The streamlined modification discussed in this webinar, Fluorescent Amplification for Next Generation Sequencing (FA-NGS), replaces PCR with real-time qPCR and SYBR Green to save time and implement single tube and single reagent QC. FA-NGS enables in situ monitoring of library amplification and replaces the need for a separate quantification step of individual libraries. Additionally, FA-NGS allows for melting curve analysis as an intermediate library quality control step to aid in troubleshooting library failures prior to sequencing.
Attendees of this webinar will learn:
- How to integrate qPCR and melting curve analysis into high-throughput NGS library preparation
- Potential applications to various library workflows
Speaker: Jennifer Chiniquy Senior Research Associate Lawrence Berkeley National Laboratory