Exploring the Stoichiometry of Macromolecular Complexes Using Multi-Signal Sedimentation Velocity Analytical Ultracentrifugation
This presentation covers advances in sedimentation velocity (SV) analysis including methods for accurately characterizing heterogeneous systems at high resolution; approaches to extract rich information from SV experiments and techniques to measure the strength of macromolecular interactions with discrete binding models to whole boundary SV experiments in analytical ultracentrifugation.
The analytical ultracentrifugation (AUC) technique is presented as a highly effective method for characterizing the structural aspects of macromolecules and as a quantitative technique that provides access to detailed thermodynamic characterization of a system under study. Also learn how AUC enables study of conformational changes and complex formation in physiologically relevant buffers. Dr. Andy Herr provides a great overview of analytical ultracentrifugation (AUC). He discusses basic theory of AUC, sedimentation velocity, sedimentation equilibrium, and more.
Webinar covering protein aggregate quantification by sedimentation velocity by Michael Stoner PhD of Amgen - PART I. AUC instrumentation & protein aggregate quantification; PART II. Size exclusion chromatography vs sedimentation velocity; PART III. Simulation as a tool for understanding results.