The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems

Speaker: Dr. Lake Paul  - Founder and President, BioAnalysis, LLC

Date: September 3, 2020


As SV-AUC becomes the go-to method for quantitation and characterization of AAV, caution must be taken during the experimental design and analysis. The heterogeneous nature of the intermediate capsids, possible self-associations, pressure effect of the hydrodynamic shape of the capsids, and even the approach to the data fitting are factors that must be carefully considered. A complete and robust understanding of the aforementioned factors along with a strong experimental design can produce reliable SV-AUC methods suitable for cGMP qualification and beyond

Learning Objectives

  1. Identify approaches to correctly analyzing AAV systems.
  2. Applying Analytical Ultracentrifugation (AUC) in a cGMP environment.
  3. Develop proper experimental design for AAV systems and extracting the correct information.