Antibody Characterization and Sample Preparation

During antibody characterization, labs will need to validate lot-to-lot reproducibility. Or, every lot they are manufacturing, the environment has been controlled and every lot is behaving as previously defined

The analytical lab is involved in many steps of lead optimization, including antibody characterization and then in every step of scale-up. When looking at characterization, the lab looks at the purity, potency, and identity of the protein.

Purity – Dosing will depend on the level of glycosylation. Are there breakdown products? Are there contaminants?
Potency- Should it be glycosylated? Does each batch contain the right combination of structures?
Identity – Does the protein have the right size, right charge, and glycosylation? 

During scale-up, manufacturing and process development are critical in validating that the antibody is behaving correctly in children and grandchildren generations. Factors such as temperature, pH, and media additives can impact cell viability and the bi-product: proteins. Each lot needs characterized to ensure critical quality attributes have been validated. 

The below resources are tools to help you characterize the binding site of your antibody to its target protein. 

Products for Antibody Characterization and Sample Prep

Optima AUC Analytical Ultracentrifuge

Analytical Ultracentrifuge

Our highest throughput centrifuge. Spin through the analytical ultracentrifuge to achieve characterization of the protein's properties. 
Biomek iSeries Automated Liquid Handlers

iSeries Automated Liquid Handlers

Automate protein characterization assays with tools such as solid phase extraction and protein digest. Automation can reduce sources of error associated with manual pipetting and improve the reliability of characterization assays. 


Research Flow Cytometers

Identify and characterize surface markers using flow cytometry. 
Duraclone B27

Research Flow Reagents

Highlight your antibody of interest with our flow reagents. 

Helpful Links

Content and Resources

Automated ELISAs—Save Time and Increase Throughput We demonstrate how the integration of the MultiWash™+ Microplate Washer and EMax® Plus Microplate Reader to a Biomek NXP Workstation facilitates the automated processing and analysis of ELISAs.