Viral Isolation & Characterization

Viral particles can be characterized in various ways depending on your intent. From isolation of viral particles, to their characterization at a genetic or whole particle level, to defining their interaction partners, each method requires a uniquely qualified tool to produce reliable, quality results.

Viral Isolation & Characterization Workflows

Nucleic Acid Isolation from Viral Samples

Extract RNA from viral samples utilizing high-performing RNAdvance Viral XP to prepare for downstream PCR protocols and data analysis, which determine positive and negative results. 
DNA Viruses

Genetic Detection of Viruses

Genetic characterization or analysis of viruses using PCR, qPCR, RT-PCR, or sequencing can be setup with automated liquid handlers that enable 21 CFR Part 11 compliance, such as the Echo 525 or Biomek i-Series, for higher throughput and improved data management.

Library Construction for Sequencing Viruses

Library construction for sequencing viruses to identify genetic variants for evolutionary and epidemiological studies using RNAClean XP for cDNA cleanup and AMPure XP for amplification cleanup.

Viral Particle Purification

High-resolution isolation and purification of intact viral particles and plasmids encoding virus/vaccine production with Optima X Series Ultracentrifuges.`

Interaction Quantification & Characterization

Characterize and quantify virus/host cell protein/nucleic acid interactions in solution with the Optima AUC.

Flow Cytometry Viral Characterization

Viral characterization by flow cytometry using the VSSC on the CytoFLEX platform will allow you to detect individual viruses and characterize their surface antigen expression.

Virion Isolation

Isolation of virions by the MoFlo Astrios EQ cell sorter for downstream characterization and study of viral composition, integrity and infectivity.