DNA and RNA Extraction from FFPE Tissue

FormaPure XL Total

Extract DNA and RNA from the same formalin-fixed paraffin-embedded tissue sample using SPRI paramagnetic bead-based chemistry.

FormaPure XL reagent kits are available in multiple kit sizes and extraction types based on your application and throughput needs. For optimum convenience, you also receive knowledgeable support for reagents and automation methods from a single vendor.

  • Scalable, single-chemistry system compatible with manual and automated processing (including sample deparaffinization and lysis)
  • Simple workflow with minimal hands-on-time
  • Demonstrated flexibility to accommodate sample input variation
  • High performance for yield, integrity and purity has been shown
  • Performance demonstrated with a variety of sample and tissue types
  • Demonstrated performance with 7x 10 μM curls

Technical Documents:

Explore From FFPE Tissue Models

FormaPure XL Total Visual Workflow

Genomics FormaPure Total XL Workflow

Product Specifications: FormaPure XL Total

Application Uses Nucleic Acid Sample Prep, Total NA Extraction, Total NA Isolation, Total NA Sequencing
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Automated Available Yes
Item Specifications Referenced C35991

Content and Resources

Agencourt FormaPure Reagent Kits Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE
Total Nucleic Acid Isolation from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues using FormaPure XL Total on a KingFisher™ Duo Prime This protocol demonstrates the FormaPure XL Total performance on the KingFisher Duo Prime purification system. Automating chemistry can reduce the risk of human error, hands-on time (HOT) and total turn-around time (TAT).
Formalin-fixed and non-paraffin-embedded tissue DNA extraction Here we demonstrated a DNA extraction protocol from formalin-fixed and nonparaffin-embedded brain tissue using FormaPure DNA XL kit. In this protocol, protein-DNA cross-links are reversed using heat
Simultaneously DNA and RNA extraction from formalin-fixed paraffin embedded (FFPE) tissue In this poster we present a streamlined NGS ready to use sample prep workflow for FFPE tissue using a product in current development, FormaPure XL Total* (Beckman Coulter Life Science). FormaPure XL Total uses SPRI (Solid Phase Reverse Immobilization) paramagnetic beads to isolate DNA and RNA simultaneously.
AMP 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples In this poster, presented at AMP 2019, we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.

Technical Documents