DNA and RNA Isolation from FFPE Tissue
Extract DNA and RNA from the same formalin-fixed paraffin-embedded tissue sample using our proprietary SPRI paramagnetic bead-based chemistry.
FormaPure reagent kits are available in multiple kit sizes and extraction types based on your application and throughput needs. For optimum convenience, you also receive knowledgeable support for reagents and automation methods from a single vendor.
- Scalable, single-chemistry system compatible with manual and automated processing (including sample deparaffinization and lysis)
- Simple workflow with minimal hands-on time
- Flexibility to accommodate sample input variation
- High performance for yield, integrity, and purity
- Validated for NGS applications with Illumina’s TruSight Tumor 170 and TruSeq Stranded RNA
- Performance demonstrated with a variety of sample and tissue types
FormaPure Total Workflow Steps
FormaPure Total is a single chemistry that isolates both DNA and RNA from single FFPE samples. The chemistry is designed and optimized for use in next-generation sequencing (NGS) and other PCR-based assays.
Flexible extraction kit suitable for manual or automated workflows. The method can be run on a variety of Biomek platforms that offer mimimal hands-on time: 30 minutes for 96 samples; 5hr 45 mins runtime for a 96-well plate.
Validated with NGS workflows. FormaPure Total isolates nucleic acids that give optimal NGS results.
FormaPure reagent kits retain the best possible nucleic acid integrity even with 10+ year old tissue blocks.
FormaPure Total Products
|Application Uses||Nucleic acid sample prep, Total NA Isolation, Total NA Extraction, Total NA Sequencing|
|Starting Sample Material||FFPE (formalin fixed paraffin embedded) tissue.|
|Nucleic Acid Sample||DNA, RNA|
|Item Specifications Referenced||C16675|
FormaPure Total for RNA Extraction
NGS performance starts with RNA integrity. Fragment analysis shows FormaPure reagent kits retain the best possible RNA integrity even with 10+ year old tissue blocks.
FormaPure RNA isolates RNA with superior integrity. Fragment analyses were performed with RNA isolated with FormaPure RNA (blue traces) and an alternative method (orange traces) from four different FFPE sample types. All extractions were performed with one 10 μm curl. DV200 values are presented for each of the chromatograms. DV200 values represent the percentage of RNA fragments > 200 nucleotides.
FormaPure Total for DNA Extraction