RNAClean XP Performance and Data

RNAClean XP provides a simple, flexible and highly reproducible method for purifying RNA and nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions. This method utilizes the Solid Phase Reversible Immobilization (SPRI) magnetic bead-based technology. It is uniquely formatted for purification of both the cDNA and RNA steps in NGS based procedures. This technique is easily performed manually in far less time than competitive methods. The RNAClean XP system doesn’t use organic solvents, vacuum filtration, or centrifugation, and delivers superior nucleic acid recovery and purity for use in downstream applications.

  • Purification of cDNA and RNA
  • Complete removal of salts, unincorporated primers and dNTPs
  • Simple automation-friendly protocol with no centrifugation, filtration or precipitation steps

 

Starting Concentration and Percent Yield

Three starting concentrations were purified using RNAClean XP. The average for each starting concentration is shown in the graph. The three starting concentrations all had a percent yield that was similar. The average recovery from the three cleanup experiments was 78% with a σ2 of 10%.

 

Genomics RNAClean XP Percent Yield

 

Visual Workflow

Genomics RNAClean XP Workflow 
  1. Bind RNA to magnetic beads
  2. Separate beads from contaminants
  3. Wash magnetic beads with 70% ethanol to remove contaminants
  4. Elute RNA from magnetic beads
  5. Transfer to new plate

 

Products to Clean RNA & cDNA

PART # NAME PREPS
A63987 RNAClean XP Kit 40 mL
A66514 RNAClean XP Kit 450 mL

 

 

Not intended or validated for use in the diagnosis of disease or other conditions.

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks are the property of their respective owners.