SPRIselect for Size Selection

Purify DNA fragments of the desired size for library preparation using our proprietary SPRI paramagnetic bead-based chemistry, which provides:

  • Tunable fragment size selection to suit specific applications
  • Predictable, consistent size selection between runs and reagent lots
  • Compatibility with manual and automated processing

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SPRIselect Left Workflow

Genomics SPRIselect Left Side Size Selection Workflow

SPRIselect Right Workflow

Genomics SPRIselect Right Side Size Selection Workflow

Product Features

Overview

SPRIselect chemistry speeds and simplifies nucleic acid size selection for fragment library preparation for next-generation sequencing.

Customizable Size Selection

SPRIselect allows tunable size selection from 150 to 800 base pairs to adjust to specific workflow needs.

Consistent Results

SPRIselect yields consistent size selections without bead calibration between reagent lots.

Flexible and Scalable

Suitable for manual or automated workflows - the SPRIselect method can be run on a variety of Biomek platforms that offer minimal hands-on time.

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-51

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz1811

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.0571

  • Used on ChIP-seq Libraries

SPRIselect vs. AMPure XP Feature Comparison

SPRIselect AMPure XP
Stable at room temperature Stable at 4oC
QC'd for size selection QC'd for recovery
Suggested for use in library construction kits Suggested for use in library construction kits

SPRIselect and AMPure XP

  • Can be substituted for each other in clean-up applications
  • Use the same sample to bead ratios
  • Are suggested for use by the most well-known library construction kit manufactures:
    • Illumnia®, Integrated DNA Technologies, Inc (IDT), Swift Biosciences, etc.
  • Both have identical workflows

SPRIselect Benefits

  • Stability at room temperature allows:
    • Start clean-up steps without calibrating to room temperature
    • Frees up 4⁰C storage space
  • Quality control for size selection allows:
    • Trust that lot to your size selection steps are accurate
    • Accurate and consistent size selection from lot to lot

1 Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Automated Size Selection using SPRIselect Beads and the Biomek FX Liquid Handler Authors: Alexandre Bélisle, Vicky Arsenault, Belisle Tir, Alfredo Staffa Sylvie LaBoissière and Alexandre Montpetit - McGill University and Génome Québec Innovation Centre - Montréal, Québec, Canada
Genomic Reagent Solutions Brochure: Next-generation sequencing (NGS) cleanup and size selection - finding the right reagent.
Customer Spotlight with Bi Lab Studying how the biochemical and transcriptional regulation and evolution of myomaker and myomixer drive muscle development, Dr. Bi and his team evaluate the newly developed EMnetik 24 microparticle processor system.
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.

Technical Documents