SPRIselect for Size Selection

Purify DNA fragments of the desired size for library preparation using our proprietary SPRI paramagnetic bead-based chemistry, which provides:

  • Tunable fragment size selection to suit specific applications
  • Predictable, consistent size selection between runs and reagent lots
  • Compatibility with manual and automated processing

Need Technical Documents or a Certificate of Analysis (COA)? Use the search bar at the top of the page and search your lot number or click below for specific SPRIselect part numbers.

SPRISelect Reagent for DNA/RNA Size Selection and Cleanup

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Explore Size Selection Models

SPRIselect Left Workflow

Genomics SPRIselect Left Side Size Selection Workflow

SPRIselect Right Workflow

Genomics SPRIselect Right Side Size Selection Workflow

Product Features

Overview
SPRIselect chemistry speeds and simplifies nucleic acid size selection for fragment library preparation for next-generation sequencing.

Customizable Size Selection
SPRIselect allows tunable size selection from 150 to 800 base pairs to adjust to specific workflow needs.

Consistent Results
SPRIselect yields consistent size selections without bead calibration between reagent lots.

Flexible and Scalable
Suitable for manual or automated workflows - the SPRIselect method can be run on a variety of Biomek platforms that offer minimal hands-on time.

Product Specifications

Application Uses Purification & Clean-up, Nucleic Acid Sample Prep, DNA Size Selection, RNA Size Selection, PCR Clean-up, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material Nucleic Acid
Automated Available Yes
Item Specifications Referenced B23318

Citations

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-51

  • Size Selection of Libraries

Kubo, M., Nishiyama, T., Tamada, Y., Sano, R., Ishikawa, M., Murata, T., . . . Hasebe, M. (2019). Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation. Nucleic Acids Research, 47(9), 4539–4553. doi:10.1093/nar/gkz1811

  • Used on cDNA Libraries

Behera, V., Stonestrom, A. J., Hamagami, N., Hsiung, C. C., Keller, C. A., Giardine, B., . . . Blobel, G. A. (2019, April 9). Interrogating Histone Acetylation and BRD4 as Mitotic Bookmarks of Transcription. Cell Reports, 27, 400–415. doi:10.1016/j.celrep.2019.03.0571

  • Used on ChIP-seq Libraries

SPRIselect vs. AMPure XP Feature Comparison

SPRIselect AMPure XP
Stable at room temperature Stable at 4oC
QC'd for size selection QC'd for recovery
Suggested for use in library construction kits Suggested for use in library construction kits

SPRIselect and AMPure XP

  • Can be substituted for each other in clean-up applications
  • Use the same sample to bead ratios
  • Are suggested for use by the most well-known library construction kit manufactures:
    • Illumnia®, Integrated DNA Technologies, Inc (IDT), Swift Biosciences, etc.
  • Both have identical work flows 

SPRIselect Benefits

  • Stability at room temperature allows:
    • Start clean-up steps without calibrating to room temperature
    • Frees up 4⁰C storage space
  • Quality control for size selection allows:
    • Trust that lot to your size selection steps are accurate
    • Accurate and consistent size selection from lot to lot

1. Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.
AMPure XP- Gold Standard for bead based, Next-Generation Sequencing (NGS) clean-up Nucleic acid purification and clean-up are mandatory for genomic applications, such as sequencing, qPCR/ddPCR/PCR, and microarrays. Maximizing recovery, consistency, and speed, AMPure XP meets the stringent needs of today’s genomic applications and minimizes the risk of losing important genetic information.
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
Impact of Clean-Up Kits on DNA Sequencing Quality and Efficiency The applications of DNA sequencing in biological research are growing. A significant driver of this growth is Next-Generation Sequencing (NGS), a modern DNA sequencing technology instrumental in achieving complete DNA sequences or genomes of humans, many animals, plants, and microbial species.
SPRI Select DataSheet The SPRISelect Reagent Kit is designed to give scientists more flexibility and control over the size selection process. With proven, industry leading Solid Phase Reversible Immobilization (SPRI)technology, this reagent provides robust, reproducible and customizable size selection.

Technical Documents