DNA Isolation from Tissue

DNAdvance

A high-throughput genomic DNA (gDNA) isolation reagent kit that enables purification of high-quality DNA from mammalian tissue samples.

  • Compatible with a variety of downstream analysis tools (e.g., PCR, qPCR, SNP genotyping and NGS)
  • Manual and automation-friendly chemistry
  • Process three 96-well plates in ~1 hour using automation

DNAdvance Genomic Reagent Kits



DNAdvance for Tissue Workflow

Genomics DNAdvance Workflow

Product Features

Overview
DNAdvance is a high throughput gDNA isolation reagent kit that enables the purification of high quality DNA from mammalian tissue samples.

Turnaround and Scale
Combining the DNAdvance kit with Biomek Workstations offers a high throughput and walk-away solution; 15 mins hands-on-time for 96 samples and 1 hr 46 mins for 96 well plate.

High Recovery of Nucleic Acids
DNAdvance consistently delivers superior recovery of nucleic acids. Isolate up to 40 ug of DNA from a variety of tissue types, e.g. brain, kidney, liver, lung, and tail.

Downstream Applications
DNA isolated with DNAdvance is compatible with a variety of downstream analysis tools such as PCR, qPCR, SNP genotyping, and sequencing.

Product Specifications

Application Uses Genomic DNA
Format Liquid
Starting Sample Material Tissue
Automated Available Yes
Item Specifications Referenced A48706

Citations

Cain-Hom, C., Splinter, E., van Min, M., Simonis, M., van de Heijining, M., Martinez, M., . . . Warming, S. (2017, January 4). Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification. Nucleic Acids Research, 45(8), e62. doi:10.1093/nar/gkw1329

  • Used to extract DNA from mouse tissue

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V., Nguyen, N. T., Zheng, Z., . . . Joung, J. K. (2015, July 23). Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature, 523(7561), 481–485. doi:10.1038/nature14592

  • Used to extract DNA from U2OS.EGFP human cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Documentation and Related Materials

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Technical Documents