DURAClone SC Hematopoietic Example Data & Gating Hierarchy

Hematopoietic progenitor cells and stem cells (HPCs, HSCs) are mainly defined by a CD45dim CD34+ phenotype. Multipotent progenitors (MPPs) can be identified by their phenotype of CD45RA- CD133+ CD38low/- CD10-, accompanied by CD90 expression in least differentiated hematopoietic cells. MPPs either differentiate into CD45RA- CD133- progenitor cells of the erythromyeloid lineage or become CD45RA+CD133+ lympho-myeloid progenitors and neutrophil and monocyte precursors. Acquisition of CD10 expression predominantly indicates commitment to the B lymphoid lineage. CD49f expression is considered indicative of a long-term repopulating fraction of HSCs.

 

Example data showing gating strategy for DURAClone SC Hematopoietic Stem Cell Antibody Panel

Identification of Hematopoietic Progenitors/stem Cells. The sample was prepared by staining bone marrow in a DURAClone SC Hematopoietic tube (Part Number C49589) following manufacturer’s instructions and acquired on a Navios EX Flow Cytometer. Data was analyzed using Kaluza Analysis software and the gating hierarchy established according to the DURAClone SC Hematopoietic Instructions For Use.