DURAClone IF T Activation Kit Example Data

Example Data in Kaluza Analysis File (download)


Analysis of activated T cells in human blood using DURAClone IF T Activation kit


Analysis of Activated T Cells.  Human blood from an apparent healthy donor was treated with DURActive 1 containing PMA, Ionomycin, and Brefeldin A (Part Number C11101). Cells were then processed with Perfix-nc Cellular Staining Preparation Kit (Part Number B10825) and stained with the DURAClone IF T Activation Cell panel (Part Number B88649) following the protocol in the Instructions for Use.  Data was acquired using the CytoFLEX S N-V-B-R Series Flow Cytometer and analyzed with Kaluza Analysis software. An exemplary gating strategy is shown.

Kaluza Analysis Tree Plot comparing T cell populations in human blood

Kaluza Analysis Tree Plot Comparison. The Tree plot shown in the gating example was created to compare TNFα+, IFNγ+ and IL-2+ expression across all CD4+ T cells and all CD8+ T cells.