Exosomes

Leading the charge in exosomes research requires pure preparations, proven reagents and accurate characterizations. Beckman Coulter stands ready to enable your analyses from the smallest particle to the deepest deconvolution. Experience the difference our leadership in exosome research can provide.

Exosomes: Moving Cancer Research Forward ~100 nm at a Time

When Professor Frederik Vannberg talks about exosomes, his enthusiasm regarding these once-neglected microvesicles and their potential role in cancer therapeutics is undeniable. Vannberg’s primary focus is the genomics of disease and his recent research to understand the role of exosomes during the immune response underscores the potential for using these particles to deliver novel therapeutics into the lymph nodes of cancer patients.

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Title	Description
Is there a difference between exosomes recovered by an isolation reagent kit and ultracentrifugation? How do I determine which protocol is "best"? {C761825C-5739-42D5-90E3-D058FB587ADC}	Options for exosome isolation.
How are exosomes visualized and characterized? {C761825C-5739-42D5-90E3-D058FB587ADC}	Identifying and characterizing exosomes.
What role do exosomes play in cancer? {C761825C-5739-42D5-90E3-D058FB587ADC}	Intracellular communication and cancer progression.
Does the type of rotor and tubes I use matter when isolating exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Rotor and tube selection for exosome isolation by ultracentrifugation.
Is there a risk that my exosomes will be damaged during ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Considerations when isolating exosomes.
Based on research, how does ultracentrifugation compare to precipitation as an isolation technique? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for achieving highest purity exosomes.
What are exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosomes - a definition.
What are typical protein, DNA, and RNA yields from exosomes isolated by ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Downstream yields from exosomes isolated by differential ultracentrifugation.
Does ultracentrifugation processing duration correlate with successful isolation of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Centrifugation processing time and successful exosome isolation.
What are exosomes made of? {C761825C-5739-42D5-90E3-D058FB587ADC}	Anatomy of an exosome.
Can diluting biofluids with PBS prior to ultracentrifugation enhance recovery of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	PBS dilution and exosome recovery.
Can I tell exosomes apart from other microvesicles? {C761825C-5739-42D5-90E3-D058FB587ADC}	Differences between exosomes and other small microvesicles.
How can I estimate where my exosome fraction of interest will be? {C761825C-5739-42D5-90E3-D058FB587ADC}	Solution for successfully finding your fraction of interest.
Are reagent kits and/or microfiltration effective alternatives to differential ultracentrifugation for isolating exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Ultracentrifugation gold standard for exosome isolation.
For which applications is it important to quantitate exosome number? {C761825C-5739-42D5-90E3-D058FB587ADC}	Quantifying exosomal protein - why and when important?
What is the function of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	The role of exosomes - normal conditions and in disease progression.
How are exosomes isolated? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for exosome isolation.
How are exosomes counted? {C761825C-5739-42D5-90E3-D058FB587ADC}	Basics of counting exosomes.
How much starting material will I need for exosome isolation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation - starting material requirements.
What medium should I use when isolating exosomes using ultracentrifugation and a density gradient? {C761825C-5739-42D5-90E3-D058FB587ADC}	Iodixanol for exosome isolation.
What cargoes do exosomes carry? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome cargoes.
Protocols for isolating exosomes frequently include differential ultracentrifugation with a density gradient. What benefits does this method offer? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation by differential ultracentrifugation with a density gradient.
Why should I consider <em>k</em>-factor when establishing a protocol for ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Answers to centrifugation questions - importance of <em>k</em>-factor.
Why don’t I see a pellet after centrifuging my exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation by ultracentrifugation - pelleted material.
Is there an isolation protocol that tends to yield the most reproducible results? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation. Reproducible results.
What is the tumor microenvironment and why is it important to tumor development? {C761825C-5739-42D5-90E3-D058FB587ADC}	Relevance of tumor microenvironment to growth.
What are the three main types of extracellular vesicles? {C761825C-5739-42D5-90E3-D058FB587ADC}	Three types of extracellular vesicles.
How useful is flow cytometry for EV isolation? What controls should be used? {C761825C-5739-42D5-90E3-D058FB587ADC}	Use and controls for extracellular vesicle isolation by flow cytometry.
Following isolation, what are the main methods for analyzing EVs? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for extracellular vesicle analysis.
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad Schwartz, Ph.D., Application Scientist I \| Randy Lockner, M.S., Centrifugation Marketing \| Randy Pawlovich, Director of Product Management & Strategy \| Beckman Coulter, Inc., Indianapolis, IN USA
To the Standardization of Exosome Isolation and Characterization {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Chad Schwartz, Ph.D. \| Zach Smith, M.S.
To the Standardization of Exosome Isolation and Characterization {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Chad Schwartz, Ph.D., Zach Smith, M.S. \| Beckman Coulter, Inc., Indianapolis, IN, 46268
Exploring Exsomes {A0D0EF0B-43F2-431C-BEB0-69F98765BDC4}	Exosomes are small (30-100 nm) secreted extracellular microvesicles involved in the transport of bioactive molecules between cells
Exosome Isolation: Density Gradient Ultracentrifugation {A0D0EF0B-43F2-431C-BEB0-69F98765BDC4}	Beckman Coulter Life Sciences
Complete Solutions for your Specific Exosome Workflow from Preparation to Analysis {749D68FB-4893-4B2D-BD6C-C662F0520F64}	Beckman Coulter provides solutions customized for each step in your specific exosome research workflow – from sample preparation to sample analysis – and can also help improve processes and assist with related challenges.
Illumina Nextera Flex for Enrichment on the Biomek i7 Hybrid Genomics Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this flyer, we describe and demonstrate the automated performance of the Illumina Nextera Flex for Enrichment assay on the Biomek i7 Hybrid Genomics Workstation
Exosome-Depleted FBS Using Beckman Coulter Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D. \| Beckman Coulter Life Sciences, Indianapolis, IN
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years
A Standardized, Automated Approach for Exosome Isolation and Characterization using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D., Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN 46268
The Basics and Vision of Exosome Research	Authors: Dr. Takahiro Ochiya and Dr. Yusuke Yoshioka \| Published 2016
Exosome Subclass Analysis	Authors: Dr. Kiyotaka Shiba, Dr. Satoshi Yamamoto and Dr. Kazuya Iwai \| Tokyo Japan
Exosome Biomarkers of Disease - Big Potential from a Little Vesicle {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	The tiny exosome (30-120 nm) is effecting a big impact on the landscape of molecular diagnostics, making it possible to diagnose and characterize disease with increasingly non-invasive methods
Small Particle {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	We explore research of small biological particles, including extracellular vesicles, algae, viruses, bacteria, and more.
Density-based Exosome Subclass Analysis {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 4 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 5 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 1 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Isolation and Characterization of Exosomes and Ectosomes	Presentation from Suresh Mathivanan at La Trobe University covers practical insights and experience relating to exosome and ectosome isolation & characterization.
Extracellular Vesicle Isolation by Flow Cytometric Sorting and Characterization by Analytical Ultra-Centrifugation and Dynamic Light Scatter	This presentation covers extracellular vesicle isolation via flow cytometric sorting and characterization by AUC and dynamic light scatter.
How Stem Cells Speak with Immune Cells	Watch this webinar from Stefan Pluchino on how stem cells signal to the host immune system
An Overview of Exosome Isolation {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief visual demonstration that explains why density gradient ultracentrifugation is considered the gold standard for isolating and purifying exosomes from body fluids
Exosomes Workflow Testimonial with Dr. Vannberg {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief discussion with Dr. Frederik Vannberg from Georgia Tech, who talks about the role exosomes play in his research on infectious diseases
Webinar: An Intro to Characterization of Biomolecules Using AUC	<span style="background-color: #ffffff; color: #5b5b5b;">Analytical Ultracentrifugation (AUC) is one of the most powerful biophysical tools used today for the characterization of biological samples ranging from small drug molecules to intact viruses, vesicles and microparticles</span>
Exosome Biogenesis and the Budding of Proteins and Viruses	Various models of exosome biogenesis have been proposed. The field however, lacks the robust mechanistic studies needed to obtain a molecular understanding of vesicle secretion. Learn how one lab has taken a cargo-based approach in which the team focuses on the cis-acting signals necessary and sufficient for the budding of specific proteins. Learn from Stephen J Gould why his work supports the hypothesis that the plasma membrane is a major site of exosome budding.
Isolation of Extracellular Vesicles: Latest Advances and Challenges	A webinar from Winston Patrick Kuo on advances and challenges in isolating extracellular (EV) vesicles. He highlights the need for streamlined, reliable and reproducible EV isolation for downstream studies.
Diversity of Extracellular Vesicles and Their Cargo in Cell-To-Cell Communication	Dr. Jan Lötvall discusses the study of extracellular vesicles and their relevance for cell-to-cell communication and how they have been implicated in several ways in disease progression. Understand the wide diversity of extracellular vesicles, with diverse morphology according to electron microscopy studies and difference in RNA and protein cargo according to bioinformatics analyses.
The Role of Exosomes in Inflammatory Disease - Pathogenesis and Treatment	This webinar covers the biology of exosomes and how they are involved in inflammatory diseases. Exosomes as biomarkers and therapeutic agents.
High-Performance Exosome Purification and Characterization via automated Density Gradient	Rapid, accurate exosome purification and sizing with density gradient, ultracentrifugation and dynamic light scattering by Joshua T. Robinson.
Characterization of Extracellular Vesicles with Nanoscale Flow Cytometry	How to set up a flow cytometer for nanoparticle analyses
Looking Under the Hood: Getting Started with the Optima AUC	n the webinar we discussed the fundamentals of analytical ultracentrifugation and looked at a few sample applications. <h3></h3>
Diversity of Cancer-Derived Extracellular Vesicles	This webinar from Dr. Dolores Di Vizio covers extracellular vesicle heterogeneity, large oncosomes and potentials for biomarker development.
Exploring Exosomes and the Tumor Microenvironment	Drs. David Lyden and Dr. Thomas Kislinger cover the topic of exosomes and the tumor microenvironment.
Proteome Profiling of the Tumor Microenvironment: Role of Human Primary Fibroblasts Derived Exosomes in Oral Cancer Progression	<p>Proteomics analysis of tumor microenvironment focused on primary fibroblast-derived exosomes in oral cancer progression presented by Simone Principe from Princess Margaret Cancer Center University Health Network.</p>
Extracellular Vesicles Delivery and RNA Translation	This webinar by Charles Lai, PhD covers visualization and tracking for extracellular delivery and RNA translation using multiplexed reporters.
Exosome Isolation	Learn why no other step is as critical to the success of exosome research as the choice of isolation technique
Exosome Subclass Analysis {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Present and Future {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 5 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 6 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 1 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Final Remarks {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Scientist Profile {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
【テクニカルノート】フローサイトメーターCytoFLEXを用いた、バイオレットレーザー側方散乱光を利用してナノ粒子を検出する方法 {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	バイオレットレーザー側方散乱光（Violet Side Scatter, VSSC）を用いてナノ粒子を検出する際のフローサイトメーターCytoFLEXの設定方法をご説明します。
Cell Signaling	Learn more about the complex communication system that governs basic cellular activities and coordinates actions such as division, differentiation, metabolism and death
Isolation and Characterization of Exosomes for Assays Approaches	Exosomes are secreted by most cells types, and can be isolated from cell culture as well as body fluids (e.g., plasma, urine, saliva); protocols for isolating exosomes have been tailored for each sample type
Exosomes: Moving Cancer Research Forward ~100 nm at a Time	When Professor Frederik Vannberg talks about exosomes, his enthusiasm regarding these once-neglected microvesicles and their potential role in cancer therapeutics is undeniable. Vannberg’s primary focus is the genomics of disease and his recent research to understand the role of exosomes during the immune response underscores the potential for using these particles to deliver novel therapeutics into the lymph nodes of cancer patients.
Targeting Efficient Drug Delivery With Exosome Nanoparticles	Research suggests exosomes may be useful for drug-delivery in the brain, though a few crucial issues must be addressed before drug-delivery via exosomes anywhere in the body can be safely implemented; learn more here
Deciphering Disease using Exosome Biomarkers	Learn how scientists are deciphering disease states with exosome biomarkers and shedding light on the major predictive value of these tiny circulating messengers
Exosomes	Learn about the huge therapeutic potential inherent in some of the tiniest microvesicles ever discovered
Apoptosis Detection	Learn why research on apoptosis (the process of programmed cell death) is playing a vital role in identifying causative or contributive factors in certain diseases
Exosomes Take Disease Modeling to the Next Level: From the Cage to the Plate	Exosomes are released not only by healthy human cells, but also by diseased cells, giving rise to liquid biopsies that have confirmed that exosomes from ill patients can contain disease-promoting nucleic acids
Simplifying Exosome Isolation	Automation: making the exosome isolation workflow work for you
Cell-Based Assays	Because cell-based assays can be an important step in many types of research, reliable pipetting that yields accurate data is more important than ever
Part 5 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
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Part 5 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 6 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
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Fundamentals of Recovery {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 3 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 4 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
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{AF9E1A10-BC29-46AA-A2A1-05BB530B6198}	Purification Method and Cautions in using Equilibrium Density Gradient Ultracentrifugal Isolation Method
Part 1 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 2 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 3 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Handling Purified Exosomes {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
Part 6 {AF9E1A10-BC29-46AA-A2A1-05BB530B6198}