Exosomes

Leading the charge in exosomes research requires pure preparations, proven reagents and accurate characterizations. Beckman Coulter stands ready to enable your analyses from the smallest particle to the deepest deconvolution. Experience the difference our leadership in exosome research can provide.

Exosomes: moving cancer research forward ~100 nm at a time

When Professor Frederik Vannberg talks about exosomes, his enthusiasm regarding these once-neglected microvesicles and their potential role in cancer therapeutics is undeniable. Vannberg’s primary focus is the genomics of disease and his recent research to understand the role of exosomes during the immune response underscores the potential for using these particles to deliver novel therapeutics into the lymph nodes of cancer patients.

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Title	Description
Exosome-Depleted FBS Using Beckman Coulter Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D. \| Beckman Coulter Life Sciences, Indianapolis, IN
A Standardized, Automated Approach for Exosome Isolation and Characterization using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D., Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN 46268
Exosome Isolation: Density Gradient Ultracentrifugation {A0D0EF0B-43F2-431C-BEB0-69F98765BDC4}	Beckman Coulter Life Sciences
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years
The Basics and Vision of Exosome Research {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	Authors: Dr. Takahiro Ochiya and Dr. Yusuke Yoshioka \| Published 2016
Exosome Subclass Analysis {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	Authors: Dr. Kiyotaka Shiba, Dr. Satoshi Yamamoto and Dr. Kazuya Iwai \| Tokyo Japan
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad Schwartz, Ph.D., Application Scientist I \| Randy Lockner, M.S., Centrifugation Marketing \| Randy Pawlovich, Director of Product Management & Strategy \| Beckman Coulter, Inc., Indianapolis, IN USA
Exploring Exsomes {A0D0EF0B-43F2-431C-BEB0-69F98765BDC4}	Exosomes are small (30-100 nm) secreted extracellular microvesicles involved in the transport of bioactive molecules between cells
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Application Scientist - Beckman Coulter, Inc., Indianapolis, IN USA \| Published 2014
Complete Solutions for your Specific Exosome Workflow from Preparation to Analysis {749D68FB-4893-4B2D-BD6C-C662F0520F64}	Beckman Coulter provides solutions customized for each step in your specific exosome research workflow – from sample preparation to sample analysis – and can also help improve processes and assist with related challenges.
To the Standardization of Exosome Isolation and Characterization {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Chad Schwartz, Ph.D., Zach Smith, M.S. \| Beckman Coulter, Inc., Indianapolis, IN, 46268
To the Standardization of Exosome Isolation and Characterization {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Chad Schwartz, Ph.D. \| Zach Smith, M.S.
Exosomes	Learn about the huge therapeutic potential inherent in some of the tiniest microvesicles ever discovered
What are the three main types of extracellular vesicles? {C761825C-5739-42D5-90E3-D058FB587ADC}	Three types of extracellular vesicles.
Following isolation, what are the main methods for analyzing EVs? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for extracellular vesicle analysis.
What is the tumor microenvironment and why is it important to tumor development? {C761825C-5739-42D5-90E3-D058FB587ADC}	Relevance of tumor microenvironment to growth.
What are typical protein, DNA, and RNA yields from exosomes isolated by ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Downstream yields from exosomes isolated by differential ultracentrifugation.
How are exosomes isolated? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for exosome isolation.
Protocols for isolating exosomes frequently include differential ultracentrifugation with a density gradient. What benefits does this method offer? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation by differential ultracentrifugation with a density gradient.
What is the function of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	The role of exosomes - normal conditions and in disease progression.
Are reagent kits and/or microfiltration effective alternatives to differential ultracentrifugation for isolating exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Ultracentrifugation gold standard for exosome isolation.
What role do exosomes play in cancer? {C761825C-5739-42D5-90E3-D058FB587ADC}	Intracellular communication and cancer progression.
How are exosomes counted? {C761825C-5739-42D5-90E3-D058FB587ADC}	Basics of counting exosomes.
Can I tell exosomes apart from other microvesicles? {C761825C-5739-42D5-90E3-D058FB587ADC}	Differences between exosomes and other small microvesicles.
Based on research, how does ultracentrifugation compare to precipitation as an isolation technique? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for achieving highest purity exosomes.
How much starting material will I need for exosome isolation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation - starting material requirements.
What medium should I use when isolating exosomes using ultracentrifugation and a density gradient? {C761825C-5739-42D5-90E3-D058FB587ADC}	Iodixanol for exosome isolation.
How are exosomes visualized and characterized? {C761825C-5739-42D5-90E3-D058FB587ADC}	Identifying and characterizing exosomes.
For which applications is it important to quantitate exosome number? {C761825C-5739-42D5-90E3-D058FB587ADC}	Quantifying exosomal protein - why and when important?
Is there a difference between exosomes recovered by an isolation reagent kit and ultracentrifugation? How do I determine which protocol is "best"? {C761825C-5739-42D5-90E3-D058FB587ADC}	Options for exosome isolation.
Can diluting biofluids with PBS prior to ultracentrifugation enhance recovery of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	PBS dilution and exosome recovery.
How can I estimate where my exosome fraction of interest will be? {C761825C-5739-42D5-90E3-D058FB587ADC}	Solution for successfully finding your fraction of interest.
Is there a risk that my exosomes will be damaged during ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Considerations when isolating exosomes.
Does ultracentrifugation processing duration correlate with successful isolation of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Centrifugation processing time and successful exosome isolation.
Why don’t I see a pellet after centrifuging my exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation by ultracentrifugation - pelleted material.
Why should I consider <em>k</em>-factor when establishing a protocol for ultracentrifugation? {C761825C-5739-42D5-90E3-D058FB587ADC}	Answers to centrifugation questions - importance of <em>k</em>-factor.
What are exosomes made of? {C761825C-5739-42D5-90E3-D058FB587ADC}	Anatomy of an exosome.
What cargoes do exosomes carry? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome cargoes.
What are exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosomes - a definition.
Does the type of rotor and tubes I use matter when isolating exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Rotor and tube selection for exosome isolation by ultracentrifugation.
Is there an isolation protocol that tends to yield the most reproducible results? {C761825C-5739-42D5-90E3-D058FB587ADC}	Exosome isolation. Reproducible results.
Isolation of Extracellular Vesicles: Latest Advances and Challenges	A webinar from Winston Patrick Kuo on advances and challenges in isolating extracellular (EV) vesicles. He highlights the need for streamlined, reliable and reproducible EV isolation for downstream studies.
Exosome Biogenesis and the Budding of Proteins and Viruses	Various models of exosome biogenesis have been proposed. The field however, lacks the robust mechanistic studies needed to obtain a molecular understanding of vesicle secretion. Learn how one lab has taken a cargo-based approach in which the team focuses on the cis-acting signals necessary and sufficient for the budding of specific proteins. Learn from Stephen J Gould why his work supports the hypothesis that the plasma membrane is a major site of exosome budding.
Diversity of Cancer-Derived Extracellular Vesicles	This webinar from Dr. Dolores Di Vizio covers extracellular vesicle heterogeneity, large oncosomes and potentials for biomarker development.
High-Performance Exosome Purification and Characterization via automated Density Gradient	Rapid, accurate exosome purification and sizing with density gradient, ultracentrifugation and dynamic light scattering by Joshua T. Robinson.
Extracellular Vesicle Isolation by Flow Cytometric Sorting and Characterization by Analytical Ultra-Centrifugation and Dynamic Light Scatter	This presentation covers extracellular vesicle isolation via flow cytometric sorting and characterization by AUC and dynamic light scatter.
Isolation and Characterization of Exosomes and Ectosomes	Presentation from Suresh Mathivanan at La Trobe University covers practical insights and experience relating to exosome and ectosome isolation & characterization.
Exploring Exosomes and the Tumor Microenvironment	Drs. David Lyden and Dr. Thomas Kislinger cover the topic of exosomes and the tumor microenvironment.
Extracellular Vesicles Delivery and RNA Translation	This webinar by Charles Lai, PhD covers visualization and tracking for extracellular delivery and RNA translation using multiplexed reporters.
How Stem Cells Speak with Immune Cells	Watch this webinar from Stefan Pluchino on how stem cells signal to the host immune system
The Role of Exosomes in Inflammatory Disease - Pathogenesis and Treatment	This webinar covers the biology of exosomes and how they are involved in inflammatory diseases. Exosomes as biomarkers and therapeutic agents.
Diversity of Extracellular Vesicles and Their Cargo in Cell-To-Cell Communication	Dr. Jan Lötvall discusses the study of extracellular vesicles and their relevance for cell-to-cell communication and how they have been implicated in several ways in disease progression. Understand the wide diversity of extracellular vesicles, with diverse morphology according to electron microscopy studies and difference in RNA and protein cargo according to bioinformatics analyses.
Proteome Profiling of the Tumor Microenvironment: Role of Human Primary Fibroblasts Derived Exosomes in Oral Cancer Progression	<p>Proteomics analysis of tumor microenvironment focused on primary fibroblast-derived exosomes in oral cancer progression presented by Simone Principe from Princess Margaret Cancer Center University Health Network.</p>
An Overview of Exosome Isolation {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief visual demonstration that explains why density gradient ultracentrifugation is considered the gold standard for isolating and purifying exosomes from body fluids
Exosomes Workflow Testimonial with Dr. Vannberg {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	A brief discussion with Dr. Frederik Vannberg from Georgia Tech, who talks about the role exosomes play in his research on infectious diseases
Exosome Biomarkers of Disease - Big Potential from a Little Vesicle {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	The tiny exosome (30-120 nm) is effecting a big impact on the landscape of molecular diagnostics, making it possible to diagnose and characterize disease with increasingly non-invasive methods
How useful is flow cytometry for EV isolation? What controls should be used? {C761825C-5739-42D5-90E3-D058FB587ADC}	Use and controls for extracellular vesicle isolation by flow cytometry.
Cell Signaling	Learn more about the complex communication system that governs basic cellular activities and coordinates actions such as division, differentiation, metabolism and death
Exosome Isolation	Learn why no other step is as critical to the success of exosome research as the choice of isolation technique
Apoptosis Detection	Learn why research on apoptosis (the process of programmed cell death) is playing a vital role in identifying causative or contributive factors in certain diseases
Exosomes: moving cancer research forward ~100 nm at a time	When Professor Frederik Vannberg talks about exosomes, his enthusiasm regarding these once-neglected microvesicles and their potential role in cancer therapeutics is undeniable. Vannberg’s primary focus is the genomics of disease and his recent research to understand the role of exosomes during the immune response underscores the potential for using these particles to deliver novel therapeutics into the lymph nodes of cancer patients.
Cell-Based Assays	Because cell-based assays can be an important step in many types of research, reliable pipetting that yields accurate data is more important than ever
Targeting Efficient Drug Delivery With Exosome Nanoparticles	Research suggests exosomes may be useful for drug-delivery in the brain, though a few crucial issues must be addressed before drug-delivery via exosomes anywhere in the body can be safely implemented; learn more here
Isolation and characterization of exosomes for assays and –omics approaches	Importance of achieving highly pure and well-characterized exosome populations for downstream assays or –omics approaches.
Deciphering Disease using Exosome Biomarkers	Learn how scientists are deciphering disease states with exosome biomarkers and shedding light on the major predictive value of these tiny circulating messengers
Targeting Efficient Drug Delivery with Exosome Nanoparticles	Learn about these tiny particles, typically 30-120 nm in size, that are secreted by most cell types and found in body fluids such as plasma, urine, saliva, serum and cerebrospinal fluid
Simplifying Exosome Isolation	Automation: making the exosome isolation workflow work for you
Exosomes Take Disease Modeling to the Next Level: From the Cage to the Plate	Exosomes are released not only by healthy human cells, but also by diseased cells, giving rise to liquid biopsies that have confirmed that exosomes from ill patients can contain disease-promoting nucleic acids
Isolation and characterization of exosomes for assays and –omics approaches	Exosomes are secreted by most cells types, and can be isolated from cell culture as well as body fluids (e.g., plasma, urine, saliva); protocols for isolating exosomes have been tailored for each sample type