CD300a (IRp60) Antibodies

The CD300a, also known as inhibitory receptor protein (IRp60), is a surface molecule of 60 kDa expressed by all human natural killer (NK) cells. The IRp60 gene, located on human chromosome region 17q25 encodes a molecule, highly O- and N-glycosylated, belonging to the immunoglobulin superfamily (Ig-SF) that displays one extracellular Ig‑like domain of the V-type, a hydrophobic transmembrane portion and a cytoplasmic tail containing ITIM motifs involved in the inhibitory function. The ligand of the CD300a is still unknown but CD300a does not appear to recognize HLA-class I molecules. Upon cross-linking, IRp60 recruits and activates SH2-containing phosphatases SHP-1 and SHP-2 which switch off the activating signaling cascade by dephosphorylating a variety of tyrosine phosphorylated proteins. Cross‑linking of IRp60 by E59.126 monoclonal antibody strongly inhibits the spontaneous cytotoxicity of NK cells as well as the NK-mediated cytolytic activity induced via different non-HLA-specific or HLA-specific activating receptors. CD300a may play a more general role in the regulation of the immune response since it is expressed on other cell types, including T lymphocyte subsets, monocytes and granulocytes.


Clone: E59.126 Isotype: IgG1 Mouse

The E59.126 antibody has been used in flow cytometry and Western blotting to characterize IRp60.

Explore CD300a Models

Content and Resources

SuperNova v428: New Bright Polymer Dye for Flow Cytometry Applications In this paper you will discover the latest generation of polymer dyes for flow cytometry applications – SuperNova fluorochromes.
CD Chart CD Chart
CE-IVD Single Color Antibodies (OUS version) CE-IVD Single Color Antibodies (OUS version)
Clinical Single Color Antibodies (US version) Clinical Single Color Antibodies (US version)
7 Tips for Multi Color Panel Design Seven Tips for Achieving the Perfect Panel for Multicolor Flow Cytometry