Protocol C00162 DuraClone IM Count

This protocol is for reference purposes only.  It may be necessary to adapt the method for specific research needs.

SAMPLE PREPARATION FOR WHOLE BLOOD (Example)

  1. Pipette 100 µL of whole blood into DuraClone IM Count Tube (Normal blood contains ~3000-11700 white blood cells per µL). Vortex at high speed for 6-8 seconds and incubate for 15 minutes at laboratory conditions (18-25°C).
  2. Add 2 mL of VersaLyse, vortex on high for 1-3 seconds and incubate for 15 minutes at laboratory conditions (18-25°C).
  3. The sample is ready for acquisition.
  4. For sample acquisition on Navios/Gallios:
  1. Prepare an unstained sample by lysing 100µL of whole blood as per step(2) and use it to set the PMT voltages for FL1 and FL4 channels by setting the X-median of the unstained sample at 0.3 for each channel.
  2. Acquire the stained tube prepared using DuraClone IM Count Tube.

Note: Keep the FL2 channel on and set PMT voltage at minimum setting