DURAClone IF T Helper Kit Example Data
Example Data in Kaluza Analysis File (download)
Analysis of Activated T Helper Cells. Human blood from an apparent healthy donor was treated with DURActive 1 containing PMA, Ionomycin, and Brefeldin A (Part Number C11101). Cells were then processed with Perfix-nc Cellular Staining Preparation Kit (Part Number B10825) and stained with the DURAClone IF T Helper Cell panel (Part Number C04666) following the protocol in the Instructions for Use. Data was acquired using the CytoFLEX LX N-V-B-Y-R-I Series Flow Cytometer and analyzed with Kaluza Analysis software. An exemplary gating strategy is shown.
Kaluza Analysis Tree Plot Comparison. The Tree plot shown in the gating example was created to compare IFNγ+, IL-17A+ and IL-4+ expression across all CD3+C/D4+ T cells.