Automated Nucleic Acid Sample Preparation Methods


Document

We offer a growing portfolio of Biomek-automated nucleic acid sample prep methods utilizing our own Agencourt reagent kits and other leading technology vendors. Each method is demonstrated to generate quality data using real-world samples by internal application scientists and customer laboratories.

Review our methods list below and supporting application overview documents to find the kits you’re currently working with in your lab or those you plan to evaluate in the future.

DEMONSTRATED METHOD
BIOMEK i7
HYBRID
BIOMEK i7/i5
MULTICHANNEL
BIOMEK i5
SPAN-8
BIOMEK FXP
HYBRID
BIOMEK FXP/NXP
MULTICHANNEL
BIOMEX NXP
SPAN-8
BIOMEK 4000
DNA AND RNA PURIFICATION AND CLEAN UP/ SIZE SELECTION/ QUANTITATION/ NORMALIZATION/ POOLING
Agilent TapeStation 2200 Plate Setup for analysis of DNA Fragments
 
Beckman AMPure XP - PCR Purification
 
 
 
 
 
 
Beckman CleanSEQ - Dye Terminator Removal
 
 
 
 
 
 
Beckman RNAClean XP - Post cDNA Purification and Post-IVT cRNA Purification
 
 
 
Beckman SPRIselect - Size Selection
 
 
 
COVARIS DNA Shearing Plate Setup for E220 Focused Ultrasonicators
 
Promega Wizard MagneSil Tfx Plasmid Purification System
 
qPCR/PCR Setup 384
 
 
 
qPCR/PCR Setup 96
 
 
 
Quantitation/Normalization
 
 
 
Roche Kapa Biosystems Library Quantification Kit - Illumina (96 and 384)
 
 
 
Sample Pooling for Multiplexing
 
NUCLEIC ACID ISOLATION
Apostle Minimax High Efficiency cfDNA Isolation kit - Isolation of cFDA from Plasma
 
 
Beckman CosMCPrep – High/Low Copy Plasmid Purification
 
 
Beckman DNAdvance - DNA Isolation from Tissue
 
 
 
 
 
Beckman Formapure XL DNA - DNA Isolation from FFPE Tissue
 
 
 
 
 
Beckman Formapure XL RNA - RNA Isolation from FFPE Tissue
 
 
Beckman Formapure XL Total -DNA and RNA Isolation from a Single FFPE Tissue Sample
 
 
Beckman GenFind v2 - DNA Isolation from Blood
 
 
 
 
Beckman GenFind v3 - DNA Isolation from Blood, Cells & Serum
 
 
 
Beckman RNAdvance Blood - RNA Isolation from Blood
 
 
 
 
Beckman RNAdvance Cell - RNA Isolation from Cells
 
 
 
Beckman RNAdvance Viral - RNA Isolation from Viral samples
 
Beckman RNAdvance Tissue - RNA Isolation from Tissue
 
 
 
 
 
Mo Bio PowerMag Soil DNA Isolation Kit
 

Method Key

 
Demonstrated Method: This indicates that the method was developed for a sample preparation kit following a vendor’s published manual protocol. Each one is tested with scientifically relevant samples and has yielded results that meet the kit vendor’s specifications either in a customer lab (customer demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated). Beckman Coulter makes no claims regarding the use or performance of these methods.
 
Illumina Qualified Methods: This indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually. This method is not an Illumina product, and Illumina does not directly support this product. Illumina makes no representations or warranties with respect to this product.
 
Method created and provided by kit vendor.



Biomek Automated Workstation Stations and methods are not intended or validated for use in the diagnosis of disease or other conditions. In some cases, method data was generated on pre-production automated workstations.

The automated methods identified are demonstrated methods only and are not validated by Beckman Coulter. Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. The demonstrated methods are available through Beckman Coulter automation field applications team.

© 2021 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All additional trademarks used throughout this document are the property of their respective owners. 21.08.2280 AUTO



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