Automated Nucleic Acid Sample Preparation Methods

We offer a growing portfolio of Biomek-automated nucleic acid sample prep methods utilizing our own Agencourt reagent kits and other leading technology vendors. Each method is demonstrated to generate quality data using real-world samples by internal application scientists and customer laboratories.

Review our methods list below and supporting application overview documents to find the reagent kits you’re currently working with in your lab or those you plan to evaluate in the future.

DEMONSTRATED METHOD		BIOMEK i7 HYBRID	BIOMEK i7/i5 MULTICHANNEL	BIOMEK i5 SPAN-8	BIOMEK FX^P HYBRID	BIOMEK FX^P/NX^P MULTICHANNEL	BIOMEX NX^P SPAN-8	BIOMEK 4000
DNA AND RNA PURIFICATION AND CLEAN UP/SIZE SELECTION/QUANTITATION/NORMALIZATION/POOLING
	Agilent TapeStation 2200 Plate Setup for analysis of DNA Fragments
	AMPure XP - PCR Purifcation
	CleanSEQ - Dye Terminator Removal
	COVARIS DNA Shearing Plate Setup for E220 Focused Ultrasonicators
	Kapa Biosystems Library Quantifcation Kit - Illumina (96 and 384)
	qPCR/PCR Setup 384
	qPCR/PCR Setup 96
	Quantitation/Normalization
	RNAClean XP - Post cDNA Purifcation and Post-IVT cRNA Purifcation
	SPRIselect - Size Selection
	Sample Pooling for Multiplexing
NUCLEIC ACID ISOLATION
	CosMCPrep – High/Low Copy Plasmid Purifcation
	DNAdvance - DNA Isolation from Tissue
	FormaPure DNA - DNA Isolation from FFPE Tissue
	FormaPure - RNA Isolation from FFPE Tissue
	FormaPure Total – Total Nucleic Acid Isolation from FFPE Tissue
	GenFind v2 - DNA Isolation from Blood
	Mo Bio PowerMag Soil DNA Isolation Kit
	RNAdvance Blood - RNA Isolation from Blood
	RNAdvance Cell - RNA Isolation from Cells
	RNAdvance Tissue - RNA Isolation from Tissue

Method Key

Demonstrated Method. This indicates that the method was developed for a sample preparation kit following a vendor’s published manual protocol. Each one is tested with scientifically relevant samples and has yielded results that meet the kit vendor’s specifications either in a customer lab (customer demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated). Beckman Coulter makes no claims regarding the use or performance of these methods.

Illumina Qualified Methods, This indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually. This method is not an Illumina product, and Illumina does not directly support this product. Illumina makes no representations or warranties with respect to this product.

Method created and provided by kit vendor.