Next-Generation Sequencing (NGS) Library Preparation

Creating libraries for next generation sequencing is a tedious process that can take from 2.5 hours to several days to complete, depending on the type of library created. Great care must be taken to keep accurate records of which samples received which adapter. Pipetting each sample and reagent by hand can lead to pipetting inconsistencies, ultimately compromising precious samples. Automating NGS library preparation can minimize these constraints associated with manual processing. Automation solutions from Beckman Coulter Life Sciences are built to alleviate your pain points, whether you need robust automation for a few samples, or need a high-throughput solution to increase your capacity.

Biomek Workstations are configurable to handle workflow needs from microliter to milliliter transfers for nucleic acid isolations from primary sample tubes, to preparing sequence-ready libraries. The capability of Echo Acoustic Liquid Handlers to enable nanoliter to microliter transfers allows for the reduction of library prep costs through reaction volume miniaturization and acoustic non-contact transfers.

 

Explore Library Construction Models

 

Other configurations are available. Request a quote or contact us for more information.

An example NGS sequencing workflow

An example NGS sequencing workflow

Biomek Workstation Solutions for NGS

Enabling higher throughput while increasing efficiency, and reducing hands-on time, Biomek Workstations provide flexibility to maximize scheduling your day. Biomek data-demonstrated methods are developed in collaboration with leading technology vendors using real-world samples. Demonstrated methods can be run start to finish with full walk-away capability, and include optional start and stop points based on the kit vendor’s recommendations.

Biomek Workstations offer scalable automation solutions for NGS sample prep workflows, including all steps involved from extraction through library construction. Biomek configurations are flexible and include capability for on-deck incubation, magnetic bead separation, and shaking, as well as optional enclosures and on-deck thermocycling, based on user needs.

 

The method/application illustrated is for demonstration only, and is not validated by Beckman Coulter. Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Biomek and Echo Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions.

Products identified are not for use in diagnostics procedures.

© 2021 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. ECHO is a trademark or registered trademark of Labcyte Inc. in the United States and other countries. Labcyte is a Beckman Coulter company.

All other product names and brands are properties of their respective owners. 

Features

Biomek Method Launcher is a secure interface for method implementation without affecting method integrity, and allows users to remotely monitor the progress of the run. The manual control options provide the opportunity to interact with the instrument, if needed. Guide Labware Setup (GLS) provides the user specific text and graphical setup instructions such as reagent volume calculations and step-by-step instructions to prepare reagents and place labware for NGS library construction.

Biomek i7 Hybrid NGS Workstation Features

  • 300 µL or 1200 µL Multichannel head with 1-300 µL and 5-1200 µL pipetting capability
  • Span-8 pod with fixed and disposable tips
  • Enhanced Selective Tip pipetting to transfer custom array of samples
  • Independent 360˚ rotating gripper with offset fingers optimize access to high density decks
  • High deck capacity with 45 positions
  • Orbital Shakers, peltiers, span-8 and 96 channel Tip washing for controlling sample processing
  • On-deck thermocycler option
  • Enclosure option
 

Biomek i5 NGS Workstation Features

  • Span-8 pod with fixed and disposable tips
  • Multichannel Enhanced Selective Tip pipetting to transfer custom array of samples
  • Independent 360˚ rotating gripper with offset fingers optimize access to high density decks
  • High deck capacity with 25 positions
  • Orbital Shakers, peltiers, and span-8 Tip washing for controlling sample processing
  • On-deck thermocycler option
  • Enclosure option
 

Biomek 4000 NGS Workstation Features

  • With both single-channel and multichannel pipette tools Biomek 4000 has a dynamic pipetting range of 1-1000 µL
  • Single Tip pipetting to transfer custom arrays of samples
  • Enhanced Gripper tool optimizes access to both on-deck and off-deck integrated solutions
  • Orbital shakers, peltiers and thermal cycling for controlling sample processing
  • Enclosure option

Specifications

Minimum Table Support Requirements 116x63cm(46x25in)
Pod and Tool Type 1μL - 300 μL 96-Channel head; 5 μL -1200 μL 96-Channel Head; 0.5 μL - 60 μL Multichannel-384 Head; Span-8 Pod
Operating System Windows® 10
Software SAMI EX scheduling software, Biomek PowerPack software, Biomek Method Launcher, DART 2.0 software, Biomek software
Display 22" controller screen
Operating Modes Semi-automated, manual, controlled by operator
Autoclavable No
Item Specifications Referenced C23339

Content and Resources

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Resource Type
Automated Sample Preparation Methods Explore our demonstrated methods for automating NGS sample prep as well as automated nucleic acid sample prep methods
A highly consistent BCA assay on Biomek i-Series The BCA (bicinchoninic acid) assay is a popular method used in quantification of proteins
A Highly Consistent Lowry Method on Biomek i-Series The quantification is based on peptide bonds of proteins reacting with copper under alkaline conditions followed by copper-catalyzed oxidation of aromatic amino acids, producing blue color
A highly consistent Bradford assay on Biomek i-Series The Bradford protein assay is a simple, fast and sensitive method that is used to measure the concentration of total protein in a sample
Automation Drug Discovery Brochure Drug discovery is all about the numbers. A sometimes overwhelming amount of numbers. Maintaining traceability of billions of cells or hundreds, maybe thousands of compounds. Library screening and hit identification. Countless hours spent at the bench, followed by dozens more hours spent mining millions of data points.