SPRI Bead Technology
Transcript
Agencourt SPRI Methodology
Solid Phase Reversible Immobilization (SPRI) technology uses paramagnetic beads to selectively bind nucleic acids by type and size.
- For high-performance isolation, purification and clean-up protocols
- Supporting applications such as qPCR, ddPCR, Sanger sequencing, next-generation sequencing (NGS) and microarrays
Nucleic Acid Immobilization
SPRI beads are directly added to sample reactions.
Nucleic acids are selectively immobilized onto SPRI beads, leaving contaminants in solution.
Contaminant Removal & Wash
A magnetic field is used to pull the microparticles out of solution.
Contaminants are aspirated and microparticles are thoroughly washed, yielding high quality nucleic acids.
Nucleic Acid Elution
Purified nucleic acids are easily eluted from the microparticles under aqueous conditions, which provides maximum flexibility for downstream applications.
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