Flow Cytometry in Cell Therapy

Flow cytometry is a critical analysis tool used in the research and development of adoptive cell therapies.

Robust, validated flow cytometry analysis is needed in many steps throughout the development process – from quality control of incoming raw materials, assessment of cell growth rates and evaluation of differentiation status, to characterization of the final product.

The inherent variability of flow cytometry experimental setup and analysis, inter-operator differences, and the constant revalidation of materials and reagents used requires a high degree of investment in developing skilled technicians for the oversight required to control the data generation and interpretation.

Simplifying Flow Cytometry Assays 

Timing is critical when developing cell therapy products. Defining your assay requirements, establishing Standard Operating Procedures (SOPs), choosing reagents and optimizing panels can be complex, time consuming and require extensive manual skilled labor.

A streamlined workflow helps ensure you get the results you trust, while keeping your flow cytometry assays simple. That’s where we come in.


For Research Use Only. Not for use in diagnostic procedures.

Data Consistency at all Stages of Drug Development 


As you develop and qualify cell therapy products, the same flow cytometric assays are often needed. You need to have confidence in how specifications have been set in order to make the assays repeatable, which is why assay validation is so critical.

When designing your flow cytometry assay, you need a robust antibody panel, avoiding inconsistencies in the reagents used or human errors in panel preparation.

With DURAClone dry, pre-formulated antibody panels, you can standardize your assays efficiently, reduce variability and get the peace of mind you deserve.

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For Research Use Only. Not for use in diagnostic procedures.


Flexible and streamlined workflows

We offer comprehensive workflow solutions that standardize your flow cytometric assays from sample preparation and data acquisition, all the way to streamlined file management and analysis.

Our set of modular solutions allow you to choose what fits your workflow at the different stages of development, allowing reproducible and standardized assays among colleagues around the world. This ultimately reduces administrative burden, costs and the need for flow cytometry experts.

The CytoFLEX Platform

cytoFLEX in use

Equipment procurement involves determining how many parameters needed, defining sample format, and sample throughput needs. The CytoFLEX platform of analyzers and sorters provide adaptability to your evolving assay needs. Whether you are testing 4, 10 or 21 colors, there’s a CytoFLEX flow cytometer for you.

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For Research Use Only. Not for use in diagnostic procedures.

The Importance of Reproducible and Automated Data Analysis

After running your flow cytometry experiment, you need to analyze the data.

Manual gating to identify groups of cells is repetitive, highly subjective and often prone to error. The Cytobank automatic gating process can help reduce variability and time to result. With it, you can reduce the time required for executing your own gating strategy and easily share gating models with other users. Researchers around the world rely on the Cytobank platform to access machine learning-assisted tools.

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Flow Cytometers and Automated Liquid Handlers

When you need confidence in the data you obtain, having control of the process, with a full understanding of the assay and its performance, is essential.

At Beckman Coulter Life Sciences, we’re committed to providing you with the right tools to simplify complex assays while improving the quality of data used to make decisions in the development of cell therapy treatments.

Our advancements in flow cytometry technology together with the seamless integration of automated liquid handling solutions remove subjectivity and manual, error-prone steps from workflows. Sample acquisition can also be automated with SCARA arm using the Automation Assistant.

With a CytoFLEX flow cytometer integrated with a Biomek i- Series liquid handler or coupled to a SCARA arm in the Automations Assistant, you can:

  • Confidently automate parts of or all your experiment
  • Build controlled experiments that generate reproducible data
  • Increase sample throughput for faster processing of high-complexity assays
Learn More About Integrated Automation

CytoFLEX Automation Assistant

Automation Assistant automatically feeds plates to the CytoFLEX cytometer for acquisition freeing up time for the end user to perform other tasks.