EMnetik Plasmid Purification System Performance Data

Plasmid Prep - Simplified

  • Plasmid recovery of 4-7 µg shown for a high copy plasmid
  • No need to handle small columns or use a single-channel pipette
  • Move samples from the lysate to the EMnetik 24 system, and don’t move them again until final elution
  • Intuitive user interface removes guesswork by providing clear, step-by-step instructions

Choose your sample input, elution and labware.

Genomics EMnetik Plasmid Purification Performance Figure 1

Figure 1

Figure 1 left: Yield of elutions from 2 different elution volume options (50 µL and 20 µL). The bars are the average of 8 replicates on the device, and the error bars are the standard deviation of the replicates. The left middle and right refer to the placement in the EMnetik 24 system; for example, the left most column of samples are averaged in the left grey bars. Figure 1 right: Yield does not vary widely when using different labware. The first three bars show the yield using PCR 8-strip tubes, and the second three bars show the yield using single PCR tubes. The bars represent the average of 8 samples; error bars indicate the standard deviation of the 8 samples. Tubes are as follows: A: Thermo Scientific AB-2005, B:VWR 93001-118, C: VWR 20170-002, D: Thermo Scientific AB-0337, E: VWR 20170-010, and F: VWR 20170-012

Plasmid extraction is comparable to manual cleanup protocols.

Genomics EMnetik Plasmid Purification Performance Figure 2

Figure 2

Figure 2: The concentration of pUC19 plasmid using EMnetik 24 and a manual bead-based chemistry were compared post extraction. The manual comparison bar is an average of 3 replicates, and the EMnetik bars are an average of 8 replicates. The error bars are the standard deviation.

EMnetik Plasmid Purification Workflow

Genomics EMnetik Plasmid Purification Workflow

EMnetik Plasmid Purification Workflow

  1. Pellet your sample
  2. Add L1 to lyse your sample
  3. Add N3 to neutralize your sample
  4. Pellet the flocculant and remove the supernatant to a new tube
  5. Add your sample to the instrument
  6. Add your EMnetik plasmid purification bind to your sample
  7. Start bind mix and separate
  8. Remove supernatant
  9. Add ethanol to wash
  10. Remove supernatant
  11. Repeat steps 5 and 6
  12. Start your ethanol dry
  13. Add your eluant
  14. Start elution mix and separate
  15. Move your final elution to your preferred labware

 

Not intended or validated for use in the diagnosis of disease or other conditions.
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