Echo 525 Acoustic Liquid Handler

For aqueous-based acoustic transfers

Explore Echo 525 Models

Liquid Handling Reimagined

Introducing the Echo 525 Liquid Handler, the first rapid acoustic liquid handler designed specifically for biochemical and genomics reagent transfer. The Echo 525 Liquid Handler and the Echo Software Applications enable scientists to spend more time looking at data. Protocol creation and implementation in the Echo Software Applications are fast and does not require extensive training. Designed for use with the Echo System, the Access Laboratory Workstation expands the Echo 525 Liquid Handler's utility and further frees up time.

Echo 525 Liquid Handler Features

Key Benefits

  • Highly precise and accurate low volume transfers enabling assay miniaturization
  • Builds assays 50–100 times faster than traditional liquid handlers giving the lab higher capacity from one instrument
  • Suite of Echo Software Applications guide creation of complex protocols for a variety of applications
  • Eliminates pipetting variances that affect assay success
  • Integrates into the Labcyte Access Laboratory Workstation for increased throughput

Build Complex Assays Quickly

The flexibility, speed, accuracy, and precision of the Echo 525 Liquid Handler enable researchers to build assays that are not possible with traditional liquid handlers. The use of acoustic sound energy removes the traditional boundaries of tip and pin tool based liquid handlers. Transfer variable fluid heights and viscosities from one source plate to enable rapid assay creation in one fluid transfer run. Say goodbye to fixed plate designs as the Echo Liquid Handler moves fluids from any well to any well enabling researchers to design the experimental plates they need to make new discoveries.

Efficiently Transfer Build Reagents with Echo Qualified Reservoir

Assay assembly and the transfer of one reagent to many wells is simplified using the Echo Qualified Reservoir. With the capability of transferring up to 2.5 µL from each micro-well, you can build your assay without requiring an instrument change – saving time and money.

Flexible and Reliable Transfer of Genomic Reagents

The Echo 525 Liquid Handler transfers a wide range of fluids during one transfer including distilled water, buffers, nucleic acids, and reagents containing up to 50% glycerol. With its versatile fluid range, transfer volume range, any well to any well transfer capability, and powerful applications software the Echo 525 Liquid Handler supports a wide range of genomic assay workflows:

  • Gene Synthesis
  • PCR and qPCR assay set-up
  • RNA and DNA NGS sequencing workflows
  • Sanger sequencing assay set-up
  • Single-cell sample preparation

Acoustic Technology Video


Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Echo 525 Acoustic Liquid Handler Specifications

Drop volume 25 nL
Volume transfer range 25 nL – 5 µL one well to one well
Transfer accuracy <10% deviation from target volume
Transfer precision <8% CV
Reagents supported PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, and antibodies
Source labware compatibility EchoQualified 384 Polypropylene, 384 Polypropylene Plus, Reservoir, Microplates
Destination labware compatibility All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-well formats 8 -16 mm in height. Use of 1536-well plates as destination plates is application dependent
Dimensions 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight 128 kg (283 lbs)
Working envelope Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature 21°C ± 5°C (70°F ± 9°F)
Vacuum House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac)
Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr (70.68 cu ft/hr), minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration No calibration needed

Content and Resources

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Illumina Nextera XT DNA Library Preparation Kit Miniaturized on Biomek i5 Multichannel 384 & Echo 525 Liquid Handlers (PDF) We have demonstrated that the automated workflow for the Nextera XT Library Prep kit from aliquoting reagents to final normalization pooling can be done in 4.5 hours for 384 samples on the Biomek i5-Echo 525 integrated system, including 35 minutes of deck set up time.
High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB® Luna® Reagents (PDF) Application Note: We show that NEB Luna qPCR reagents offer strong performance in high-throughput qPCR applications and are readily compatible with automated reaction setup, here using the Beckman Echo 525 acoustic liquid handler.