Daily Maintenance and Cleaning

Daily cleaning, changing out sample line. 

Daily Clean

Daily Clean should be performed during instrument startup and instrument shutdown to clean the sample line. After sampling an excessively large sample or a sample that can easily clog the sample probe, it is recommended to perform the Daily Clean procedure. Daily Clean can also be used to remove residual sample from previous tubes. For more detailed instructions, refer to Chapter 3 in the Instructions for Use document. 

  1. Open the CytExpert software and confirm that the instrument is connected and that it has already been initialized. 
  2. Select Daily Clean in the Cytometer menu.
  3. Add 2 mL of FlowClean solution to an unused sample tube.
  4. Add 3 mL of DI water to an unused sample tube.
  5. Insert the sample tube with 2 mL of FlowClean solution into the sample holder and select Run. 
    NOTE: The default cleaning time is 3 minutes.
  6. Remove the Flow Clean tube.
  7. Insert the sample tube with 3 mL of DI water into the sample holder and select Run to perform the second step of the cleaning process. NOTE: The default cleaning time is 5 minutes.
  8. After the process has been completed, remove the sample tube and close the Daily Clean Window.

Daily Clean [With Plate Loader]

Daily Clean should be performed during instrument startup and instrument shutdown to clean the sample line. After sampling an excessively large sample or a sample that can easily clog the sample probe, it is recommended to perform the Daily Clean procedure. Daily Clean can also be used to remove residual sample from previous tubes.

  1. Open the CytExpert software and confirm that the instrument is connected and that it has already been initialized.
  2. Select Daily Clean in the Cytometer menu. The Daily Clean window appears. The plate loader automatically ejects the plate holder stage.
  3. Follow the on screen software prompts and select the desired wells for cleaning agent and deionized water.
    IMPORTANT You must select at least one cleaning solution well and one water well.
    1. Select the desired wells for the cleaning agent and select Set As Cleaning Agent Well.
    2. Select the desired wells for the deionized water and select Set As Deionized Water Well.
      NOTE To deselect water wells, select the desired well and select Set As Empty Well.
    1. Select the Turn off cytometer after daily clean checkbox to automatically shutdown the cytometer after Daily Clean is finished. [CytoFLEX LX Only]
  4. Select Start to start the cleaning procedure. The message Please confirm that the correct plate is placed properly and press OK appears. Select OK.
  5. Select Close.

 

Tips & Tricks If you don't use your CytoFLEX every day consider filling the cleaning solution bottle with a smaller amount of Contrad 70.

 

Cleaning for Small Particle

Debris from samples can build up in the sample probe, and later break away during acquisition. This debris is mostly visible below the range of 100nm PS particles, so this will not be apparent with less sensitive flow cytometers.

  1. This debris can be cleaned by running a panel of Bleach, FlowClean, and then Water to flush. What’s the mix?
  2. Repeat if necessary. If major noise remains after 2 rounds, it may be in the buffer or sheath.

Additional Notes

  1. If you have previously identified the optimal threshold for your settings, then you can bypass Step E. The CytoFLEX results and settings are very consistent, and any previously identified optimal settings will still be optimal in the future, all things being equal.
  2. If the optimal threshold has not been identified, assessing the cleanliness at any setting can be a futile effort. It is important to know where to look.
  3. Regular CytoFLEX Sheath works very well for small-particle detection on the CytoFLEX. Higher-purity sheath is not necessary. The CytoFLEX Sheath also contains an antimicrobial agent that will prevent instrument contamination.