Why should I use a vector for gene transfer?
However, to best ensure nucleic acid uptake by the cell and incorporation into the target genome, the target nucleic acid sequence is attached to a vector. Vectors are DNA molecules containing a foreign DNA insert which are used to transport and deliver said payload to the target cell’s genome for incorporation. Commonly used vectors include bacterial plasmids, lipids, nanoparticles, and viruses.1,2
1. M.S. Al-Dosari and X. Gao, “Nonviral Gene Delivery: Principle, Limitations, and Recent Progress,” AAPS J 11(4): 671, 2009.
2. I.M. Verma and N.B. Somia, “Gene therapy -- promises, problems and prospects,” Nature 389(6648): 239-242, 1997.