How feasible is simultaneous staining of surface and intracellular antigens using DURAClone antibody panels?

DURAClone IF Holding Reagent Vial
The single step-staining of intracellular and surface antigens is done in sequential steps using PerFix-nc sample processing solutions, an innovative cell permeabilization kit that simplifies intracellular staining procedures and makes them more reproducible.