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Step-by-step instructions for generating a compensation matrix in CytExpert for CytoFLEX, the acquisition and analysis software used in the CytoFLEX Flow Cytometry platform.
Describes fundamentals of fluorescence spillover and the process of compensation in flow cytometry. CytExpert software compensation setup and multicolor flow cytometry set up is shown along with a demonstration of gain independent compensation available in the CytoFLEX flow cytometry platform.
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