Next generation Sequencing Library Preparation

Automated Liquid Handling Solutions

Next generation sequencing (NGS), or massively parallel, high-throughput sequencing technology, continues to play an integral part in genomic research. Although a basic NGS workflow consists of just three steps—library preparation, sequencing, and data analysis—library preparation is a tedious, labor-intensive process that can be crucial to the success of your sequencing data.

Cost Saving Through Reducing Library Prep Volumes

As the cost of sequencing has gone down the cost of library prep has become a bottleneck, especially in high-throughput applications such as microbial or single-cell sequencing. One way to reduce the library prep cost is reducing or miniaturizing the reaction volumes.

Echo Liquid Handlers enable library preparation in low microliter volumes for a range of sequencing methods including Sanger, 16S and NGS – to dramatically reduce reagent costs, sample usage, and eliminate steps — all while maintaining/improving data quality and increasing the throughput.

Reduce Library Prep Costs 100-Fold

Using the Echo Liquid Handler for NGS library preparation versus conventional liquid handlers allows for significant reduction in reaction volumes (~100-fold) and therefore reducing library prep costs.

Comparison of Liquid Handling Methods

   Manual Pipetting Echo Liquid Handler
 Amount of DNA  50 ng 0.06 - 2.0 ng 
 DNA Volume (Rxn)  25 µL  200 nL
 Library prep volume (Rxn)  25 µL  300 nL
 Total volume  50 µL  0.5 µL
 Reactions per kit  96  9600
 Cost per reaction  $72.91* $0.73* 

* Based on list pricing.

Echo 525 Acoustic Liquid Handler

Reduce Transfer Volumes by 75% or More

When NGS sequencing programs scale, the cost of library preparation is a limiting factor. With the Echo Liquid Handler's low-volume acoustic transfer capability, researchers can significantly increase the number of libraries generated while maintaining current budgets.

Normalize and Pool Libraries for Sequencing with Greater Accuracy and Precision in Less Time

Echo Liquid Handlers can rapidly pool and normalize oligos or DNA libraries from any well of a microplate. With average transfer times of no more than a few seconds per well, Echo Liquid Handlers can reduce pooling times by more than 80% compared to traditional liquid handlers. Since Echo systems can transfer volumes as little as 25 or 2.5 nL, high concentration libraries do not have to be diluted prior to transfer. This capability results in simultaneous normalization while pooling. Furthermore, by avoiding tip-based sample dilution, the propagation of errors from sample retention by tips is eliminated. This drastically improves the concentration accuracy and precision of all pooled libraries.

Key Benefits

  • Reduced library prep cost through efficient assay miniaturization
  • Preserved or improved data quality with reaction volumes as low as 250 nL
  • Fast and simultaneous normalization and pooling of libraries due to wide dynamic range of the Echo
  • Integrated systems and software tailored for genomic reactions

Biomek Genomic Workstations

Automated solutions can start at nucleic acid isolation, then continue through steps like NGS library construction, and result in analysis-ready libraries. We deliver demonstrated methods enabling faster implementation of your genomic workflows.

  • Demonstrated methods are developed for a sample preparation kit following a vendor’s published manual protocol.
  • These methods are tested with scientifically relevant samples and yield results that meet the kit vendor’s specifications either in a customer or vendor lab (customer/vendor demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated).
  • Demonstrated methods also include a growing number of Illumina®-qualified NGS methods. Illumina-qualified methods indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually.

Miniaturized Illumina Nextera XT Library Prep Using an Integrated Biomek i5 and Echo 525 Liquid Handling Workstation

Nextera XT NGS Library Prep

Sequencing Nextera XT NGS Library Prep

Genomic Reagents Portfolio

Our reagents are powered by Solid Phase Reversible Immobilization or SPRI technology, which uses SPRI paramagnetic beads to selectively bind nucleic acids by size. It’s ideal for nucleic acid extraction from cells, tissue, blood and even challenging formalin-fixed, paraffin-embedded (FFPE) samples.
* Low-cost, high-throughput sequencing of DNA assemblies using a highly multiplexed Nextera process. Shapland et al. ACS Synth. Biol., 2015
Additional Resources

Video: Echo Liquid Handling for Next-Gen Sequencing

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