Standardized Results - Clinical Vignettes

This casebook has been designed to assist in the analysis of flow cytometric immunophenotyping data generated using Beckman Coulter’s ClearLLab 10C Panels.

Complete the form and download your free copy of this invaluable flow cytometry casebook for Leukemia & Lymphoma* analysis.

*Non-Hodgkin's lymphoma only

This 70-year-old male presents with circulating blasts. A peripheral whole blood sample is submitted for flow cytometric immunophenotyping using ClearLLab 10C Panels.

Compared with normal B cells, the expression of CD5, decreased CD20, and lambda light chain restriction of low intensity are aberrant.

The immunophenotype of the abnormal population is in keeping with abnormal immature T cells, i.e. T-lymphoblasts.

Compared with normal mature T cells, the decreased expression of CD2, CD5 and CD7 with increased CD16 and CD56 is aberrant.

Compared with normal CD34-positive progenitors, the near-uniform expression of CD7 and decreased expression of CD13 is aberrant.

Compared with normal mature monocytes, the decreased expression of CD13 and CD14 is aberrant.

Complete the form and download your free copy of this invaluable flow cytometry casebook for Leukemia & Lymphoma* analysis.

*Non-Hodgkin's lymphoma only

Compared with normal B cells, the expression of CD5, decreased CD20, and lambda light chain restriction of low intensity are aberrant.

Compared with normal B cell precursors, the expression of increased CD10, dim CD13, dim CD33, CD34 on a subset, and CD123 are aberrant.

Compared with normal peripheral blood, the presence of an immature T cell population in the peripheral blood is aberrant, with co-expression of CD4 and CD8, mildly decreased CD5, and dim CD45.

The blasts are in the CD45dim gate and express CD33, CD34, CD117 and HLA-DR. The blasts also abnormally express CD10 and CD56 (in a subset), while negative for CD19.