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AQUIOS Lysing Reagent Kit consists of two reagents (Reagent A and Reagent B) used by AQUIOS flow cytometers to prepare whole blood samples for analysis of white blood cells. Reagent A lyses red blood cells in whole blood samples sufficiently to perform monoclonal antibody panel analysis without interference from red blood cells. Reagent B slows the lysing reaction sufficiently to allow analysis without damage to white blood cells. The lysing reagents are placed onboard the system and are barcoded, which allows the system to detect their presence and position. High volume users can load multiple containers and the system will automatically move from one container to the next as needed.
IMMUNOPREP Reagent System
The IMMUNOPREP Reagent System is a rapid, no-wash, standardized, whole blood preparation procedure maximizing sample to sample, and laboratory to laboratory reproducibility. The IMMUNOPREP reagent lysing action reduces cell stress and minimizes cell shrinkage while maintaining cellular integrity and morphology. Separation of leukocyte cell populations of interest from red blood cell ghosts and debris is maximized. Scatter characteristics are optimized for superior cell type resolution.
IOTest 3 Lysing Solution
IOTest3 Lysing Solution is an ammonium chloride-based erythrocyte lysing solution designed for flow cytometry. This reagent is provided as a 10X-concentrated mixture of ammonium chloride, potassium bicarbonate and ethylenediamine tetraacetic acid (EDTA). IOTest3 Lysing Solution allows whole blood or bone marrow specimen preparation by erythrolysis during immunophenotyping procedures. A wash post lysis is required to yield optimal results.
OptiLyse No-Wash Lysing Solutions
Both OptiLyse C and B are erythrolytic reagents intended for lysing human red blood cells following direct immunofluorescence staining of whole blood for flow cytometry. OptiLyse C is a ready-to-use lysing solution optimized for use on Beckman Coulter instruments. OptiLyse B is a ready-to-use lysing solution optimized for use on BD Biosciences* instruments.
VersaLyse Lysing Solution
VersaLyse Lysing Solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse / wash, wash / lyse and lyse / no-wash procedures with undiluted or diluted samples.
Whole Blood Lysing Reagents
The 100-test lysing kit (part number 6602764) provides Immuno-Lyse, Fixative and Stabilizer for preparation of whole blood for analysis by fluorescence microscopy or flow cytrometry. The 300-test lysing kit (part number 6603152) provides Immuno-Lyse and Fixative for preparation of whole blood for analysis by flow cytometry only.
Explore Lysis without Permeabilization Models
Please view Technical Documents for Lysis without Permeabilization
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