Automated Next-Generation Sequencing Sample Preparation Methods


Document

We partner with customers and leading technology vendors to develop and deliver the best solutions. Our vast portfolio of Biomek-automated next-generation sequencing sample prep methods are demonstrated to generate quality data using real-world samples, and includes a growing number of Illumina®-qualified NGS methods.

Review our methods list below and supporting application overview documents to find the kits you’re currently working with in your lab or those you plan to evaluate in the future.

DEMONSTRATED METHOD
BIOMEK i7
HYBRID
BIOMEK i7/i5
MULTICHANNEL
BIOMEK i5
SPAN-8
BIOMEK FXP
HYBRID
BIOMEK FXP/NXP
MULTICHANNEL
BIOMEX NXP
SPAN-8
BIOMEK 4000
CANCER PANELS
Illumina AmpliSeq Cancer HotSpot Panel v2
 
Illumina AmpliSeq Cancer HotSpot Panel v3
 
Illumina AmpliSeq Focus Panel
 
Illumina TruSight Oncology 500 DNA/RNA
 
Illumina TruSight Oncology 500 HT
 
Illumina TruSight Cancer
 
Illumina TruSight Tumor 15
 
Illumina TruSight Tumor 170
 
 
Illumina TruSight Tumor 500
 
NEB NEBNext Direct Cancer HotSpot Panel
 
 
TruSight RNA Pan Cancer
 
DNA SEQUENCING
ATAC-Seq
 
Illumina AmpliSeq for Illumina NGS
 
Illumina DNA PCR-Free Prep
 
Illumina ForenSeq
 
Illumina Nextera DNA Flex Library Prep Kit
 
Illumina Nextera XT
 
 
Illumina TruSeq Custom Amplicon Low Input Kit
 
Illumina TruSeq DNA PCR-Free
 
 
 
 
Illumina TruSeq Nano DNA
 
 
 
 
NEB NEBNext Ultra DNA for Illumina NGS (ChIP-seq and HLA)
 
NEB NEBNext Ultra II DNA Kit for Illumina NGS
 
 
NEB NEBNext Ultra II FS DNA
 
NuGEN Ovation Ultralow Library System v2
 
NuGEN Ovation Rapid Library System (PCR-Free)
 
Roche KAPA HyperPrep Library Prep Kit for Illumina NGS
 
 
 
 
 
Roche KAPA HyperPlus Library Prep Kit for Illumina NGS
 
 
 
 
 
Swift Biosciences Accel - NGS 2S Plus DNA Library Kit for Illumina NGS
 
Takara SMARTer ThruPLEX DNA-seq
 
Twist Library Preparation Kit (Mechanical & enzymatic fragmentation)
 
 
HLA TYPING
GenDx NGSgo
 
 
Illumina TruSight HLA Sequencing Panel v2
 
Immucor MIA FORA Flex HLA
 
 
 
 
 
Omixon Holotype HLA X2
 
RNA SEQUENCING
Illumina AmpliSeq for Illumina NGS
 
Illumina RNA Prep with Enrichment
 
Illumina TruSeq RNA v2
 
Illumina TruSeq RNA Exome
 
 
Illumina TruSeq Stranded mRNA
 
 
Illumina TruSeq Stranded Total RNA
 
 
Lexogen QuantSeq 3' mRNA -Seq Library Prep Kit
 
NEBNext ARTIC SARS-CoV-2 FS Express
 
NEB NEBNext Small RNA Kit for Illumina NGS
 
NEB NEBNext Ultra II Directional RNA Library Kit for Illumina NGS
 
 
NuGEN Ovation Universal RNA-Seq
 
NuGEN Trio RNA-Seq
 
NuGEN Universal Plus mRNA-Seq
 
Roche KAPA mRNA HyperPrep Kit
 
Roche KAPA RNA HyperPrep Kit with RiboErase (HMR)
 
SINGLE-CELL SEQ
10X Genomics Single Cell 3’ kit- Library Preparation
 
NEB NEBNext Single Cell/ Low Input RNA Library Prep Kit
 
Takara SmartSeq HT
 
TARGET/EXOME CAPTURE
Agilent HaloPlex Target Enrichment - Ion Torrent
 
 
 
Agilent SureSelect XT
 
 
IDT xGen Hybridization and capture of DNA Libraries
 
Illumina Nextera DNA Exome Kit
 
Illumina Nextera Flex Enrichment
 
Illumina Nextera Rapid Capture
 
 
Illumina TruSeq Exome
 
 
NEBNext Direct Genotyping Solution
 
NuGEN Allegro Targeted Genotyping
 
Roche Nimblegen SeqCap EZ for Illumina NGS
 
Twist Fast Hybridzation and Wash
 
Twist Standard Hybridization and Wash
 

Method Key

 
Demonstrated Method: This indicates that the method was developed for a sample preparation kit following a vendor’s published manual protocol. Each one is tested with scientifically relevant samples and has yielded results that meet the kit vendor’s specifications either in a customer lab (customer demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated). Beckman Coulter makes no claims regarding the use or performance of these methods.
 
Illumina Qualified Methods: This indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually. This method is not an Illumina product, and Illumina does not directly support this product. Illumina makes no representations or warranties with respect to this product.
 
Method created and provided by kit vendor.
 
TruSight Qualified method: These methods are co-developed by Illumina and the automation partner, undergo verification testing at Illumina and are alpha tested at a user site. The methods are installed and supported by the partner, but Illumina FAS will offer method training and will provide front line support. Technical support will also offer front line support in order to determine if the customer issue is the method/automation or the kit.



Biomek Automated Workstation Stations and methods are not intended or validated for use in the diagnosis of disease or other conditions. In some cases, method data was generated on pre-production automated workstations.

The automated methods identified are demonstrated methods only and are not validated by Beckman Coulter. Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. The demonstrated methods are available through Beckman Coulter automation field applications team.

© 2021 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All additional trademarks used throughout this document are the property of their respective owners. 21.08.2280 AUTO



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