Automated Next-Generation Sequencing Sample Preparation Methods

We partner with customers and leading technology vendors to develop and deliver the best solutions. Our vast portfolio of Biomek-automated next-generation sequencing sample prep methods are demonstrated to generate quality data using real-world samples, and includes a growing number of Illumina®-qualified NGS methods.

Review our methods list below and supporting application overview documents to find the kits you’re currently working with in your lab or those you plan to evaluate in the future.

DEMONSTRATED METHOD
BIOMEK i7
HYBRID
BIOMEK i7/i5
MULTICHANNEL
BIOMEK i5
SPAN-8
BIOMEK FXP
HYBRID
BIOMEK FXP/NXP
MULTICHANNEL
BIOMEX NXP
SPAN-8
BIOMEK 4000
CANCER PANELS
Illumina TruSight® Cancer
Illumina TruSight® Tumor 15
Illumina TruSight® Tumor 170
Illumina TruSight® Tumor 500
NEB NEBNext Direct Cancer HotSpot Panel
TruSight RNA Pan Cancer
DNA SEQUENCING
ATAC-Seq
Illumina AmpliSeq for Illumina NGS
Illumina ForenSeq
Illumina Nextera® DNA Flex Library Prep Kit
Illumina Nextera® XT
Illumina TruSeq® Custom Amplicon Low Input Kit
Illumina TruSeq® DNA PCR-Free
Illumina TruSeq® Nano DNA
NEB NEBNext® Ultra DNA for Illumina NGS (ChIP-seq and HLA)
NEB NEBNext® Ultra II DNA Kit for Illumina NGS
NEB NEBNext® Ultra II FS DNA
NuGEN Ovation Ultralow Library System v2
NuGEN Ovation Rapid Library System (PCR-Free)
Roche KAPA HyperPrep Library Prep Kit for Illumina NGS
Roche KAPA HyperPlus Library Prep Kit for Illumina NGS
Swift Biosciences Accel - NGS® 2S Plus DNA Library Kit for Illumina NGS
HLA TYPING
GenDx NGSgo®
Illumina TruSight® HLA Sequencing Panel v2
Immucor MIA FORA Flex HLA
Omixon Holotype HLA X2
RNA SEQUENCING
Illumina AmpliSeq for Illumina NGS
Illumina TruSeq® RNA v2
Illumina TruSeq® RNA Exome
Illumina TruSeq® Stranded mRNA
Illumina TruSeq® Stranded Total RNA
Lexogen QuantSeq 3' mRNA -Seq Library Prep Kit
NEB NEBNext® Small RNA Kit for Illumina NGS
NEB NEBNext® Ultra II Directional RNA Library Kit for Illumina NGS
NuGEN Ovation Universal RNA-Seq
NuGEN Trio RNA-Seq
NuGEN Universal Plus mRNA-Seq
Roche KAPA™ mRNA HyperPrep Kit
Roche KAPA™ RNA HyperPrep Kit with RiboErase (HMR)
SINGLE-CELL SEQ
NEB NEBNext Single Cell/ Low Input RNA Library Prep Kit
Takara SmartSeq® HT
TARGET/EXOME CAPTURE
Agilent HaloPlex Target Enrichment - Ion Torrent
Agilent SureSelect XT®
IDT xGen Exome Research
Illumina Nextera® DNA Exome Kit
Illumina Nextera® Flex Enrichment
Illumina Nextera® Rapid Capture
Illumina TruSeq® Exome
NuGEN Allegro Targeted Genotyping
Roche HyperCap Workflow v2.0
Roche Nimblegen SeqCap EZ® for Illumina NGS

Method Key

 
Demonstrated Method. This indicates that the method was developed for a sample preparation kit following a vendor’s published manual protocol. Each one is tested with scientifically relevant samples and has yielded results that meet the kit vendor’s specifications either in a customer lab (customer demonstrated) or in a Beckman Coulter Life Sciences Lab (Beckman demonstrated). Beckman Coulter makes no claims regarding the use or performance of these methods.
 
Illumina Qualified Methods, This indicates that Illumina’s analysis of libraries prepared with the Biomek-automated method has shown the libraries to perform comparably to those prepared manually. This method is not an Illumina product, and Illumina does not directly support this product. Illumina makes no representations or warranties with respect to this product.
 
Method created and provided by kit vendor.
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