Gain Independent Compensation

A feature in CytExpert for CytoFLEX software is called Gain Independent Compensation. In a CytExpert compensation matrix, the gain value is recorded along with the spillover values. When the gain is adjusted during the experiment, the compensation matrix is updated to reflect the change in the spillover values.


Clip from CytExpert Compensation Workflow Video demonstrating Gain Independent Compensation.

This feature is made possible by the types of detectors used and how they were implemented in the CytoFLEX platform. Due to the reproducible semiconductor manufacturing process, the gains of the detectors used in the CytoFLEX platform can be calibrated for a linear response. Measured intensities are linear to the detector gain setting across a wide range of gain settings. This means the fluorescence measurement increases or decreases linearly based on the gain adjustment. (Each instrument has its own gain/fluorescence curve for each detector).

Gain Independent Compensation is one of the features that helps users new to flow cytometry implement multicolor panels into their experimental programs and laboratories.


CytoFLEX Flow Cytometer Multicolor Workflow

CytoFLEX Flow Cytometer Multicolor Workflow. In the CytoFLEX flow cytometer, the Gain Independent Compensation means that the compensation matrix obtained at one gain setting can be used for experiments at different gain settings. The software automatically recalculates spillover values in real time as the gains are adjusted. Download Complete Infographic.


For Research Use Only. Not for use in diagnostic procedures.

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