Instrument Startup



Instrument Preparation

  1. Ensure all system connections are connected correctly.

  2. CytoFLEX Instrument Preparation: Ensure all system connections are connected correctly
  3. Ensure that all of the tubing and the cables are connected to the instrument according to the color code.

    CytoFLEX Instrument Preparation: Ensure that all of the tubing and the cables are connected to the instrument according to the color code
    1. Waste level sense. Connects to the waste liquid sensor cable.
    2. Flow cell waste out. Connects to the flow cell waste tubing.
    3. Sheath fluid level sense. Connects to the sheath fluid sensor cable.
    4. Waste out. Connects to the waste liquid tubing.
    5. Sheath return. Connects to the sheath fluid tubing.
    6. Sheath fluid in. Connects to the sheath fluid tubing.
  4. Verify that the USB configuration key has been connected to a USB port.
    Caution
    Risk of instrument damage. Remove the sheath fluid container from the Fluid Container holder and fill away from the instrument to prevent spills that could damage the instrument circuitry.
  5. Remove the sheath fluid container from the Fluid Container holder and fill the sheath fluid container with the supplied sheath fluid.
  6. If necessary, remove the right side cover and fill the Deep Clean solution with a mixture of 1 part Contrad 70 and 1 part DI water.
    Warning
    Risk of chemical injury from bleach. To avoid contact with the bleach, use barrier protection, including protective eyeware, gloves, and suitable laboratory attire. Refer to the Safety Data Sheet for details about chemical exposure before using the chemical.
  7. Empty the waste container if necessary. Add 400 mL of 5 to 6% bleach to the waste container.

Instrument Start Up

  1. Turn on the power switch on the back cover of the instrument, located just above the power cable.
  2. Log on to the computer and double-click CytExpert logo to start CytExpert.
    1. Ensure that the Connected icon on the Status Bar near the bottom-left side of the display is green.
      CytoFLEX Instrument Startup: Ensure that the Connected icon on the Status Bar near the bottom-left side of the display is green.
    2. If the icon is not green, ensure that the instrument USB is securely connected to the computer and restart the computer.
  3. Select System Startup Procedure in the Cytometer menu, click Initialize and follow the software prompts.