MoFlo Astrios EQ Optics
Accelerate cell sorting with the first true 32-bit high-resolution 5-decade multi-channel system
Excitation Optics—Laser Palette
- Seven discrete laser interrogation points
- Seven pinholes
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Simultaneously measure crucial particle characteristics in more than three orders of magnitude. |
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Wavelength |
Laser Power |
Elliptical shape |
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355nm |
100 mW |
Flat-Top shape |
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405nm |
55 mW |
Flat-Top shape |
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488nm |
200 mW |
Flat-Top shape |
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532nm |
150 mW |
Flat-Top shape |
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561nm |
200 mW |
Flat-Top shape |
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592nm |
200 mW |
Flat-Top shape |
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642nm |
100 mW |
Flat-Top shape |
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Standard Filter Sets Optimized per Laser Wavelength |
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355nm |
PI, Hoechst (red and blue), DAPI |
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405nm |
SSC, Cascade Blue*, Pacific Blue*, Cascade Yellow* |
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488nm |
SSC, FITC, PE, ECD (PE-Texas Red*), PE-Cy5/PerCP, PE-Cy5.5, PE-Cy7 |
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532nm |
SSC, PE, PE-Alexa* 610, PE-Alexa 647, PE-Cy5.5, PE-Alexa 700 |
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561nm |
SSC, mCherry, DSRed, mPlum, HCRed |
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592nm |
SSC, Texas Red, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750 |
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642nm |
SSC, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750 |
*Certifications adhere to local Regulatory Standards.
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Emission Optics |
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Resolution |
Resolves minimum 0.2µm diameter particles in scatter from background. |
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Fluorescence Sensitivity |
Fluorescence sensitivity of < 125 MESF for FITC and <110 MESF for PE using Spherotech 8-peak beads using 488nm laser. |
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Optical Parameters |
Up-to 44, including two FSC |
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Fluorescence half height CV precision |
FITC<2.4%, PE <2.4%, PE-TR <2.6%, PE-Cy5 <3.2%, PE-Cy7 <4.3%, APC <2.3%, APC-Cy7 <2.8% (1x 106 SPHERO™ Ultra Rainbow beads at sample flow rate of 6µl/min, 100-120 EPS) |