Following isolation, what are the main methods for analyzing EVs?

FAQs Scientific

Researchers first determine the protein concentration (BSA assay), then antibody confirmation (i.e. CD63) using either western blot or capture beads. Populations are also characterized by size and dispersity using dynamic light scatter (DLS) and finally, a high-resolution analysis by either nanotracking analysis or analytical ultracentrifugation (AUC).