How useful is flow cytometry for EV isolation? What controls should be used?

Дозирование жидкости в стеклянные пробирки

Researcher can use flow cytometric sorting for single EV isolation which will generate high purity but lower yield than ultracentrifugation. Several controls are required to confirm the visible population contains EVs including, sample media, unstained EVs, stained EVs, stain solution with no EVs, and fluorescence and scatter calibration standards.