Agencourt RNAdvance Blood for Biomek 4000 Laboratory Automation Workstation
The RNAdvance Blood total RNA purification kit utilizes a patented Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology to isolate total RNA from PAXgene preserved blood. The protocol can be performed in both 96-well plate and single tube formats. Purification begins with lysis and protein digestion. Following lysis, the RNA is immobilized onto the magnetic particles allowing separation from contaminants using a magnetic field. The RNA is then treated with DNase and the contaminants rinsed away using a simple wash procedure. The RNAdvance Blood kit is amenable to automation as it utilizes magnetic separation, eliminating the need for vacuum filtration or centrifugation. The following protocols are used for the isolation of total RNA from 400 μL of PAXgene preserved blood per well in 96-well and 2 mL tube formats. Set up for the Biomek® 4000.