Analytical Ultracentrifugation in the Biopharmaceutical Industry
The analytical ultracentrifugation (AUC) technique is presented as a highly effective method for characterizing the structural aspects of macromolecules and as a quantitative technique that provides access to detailed thermodynamic characterization of a system under study. Also learn how AUC enables study of conformational changes and complex formation in physiologically relevant buffers.
Covers the utility of fractionation in the investigation of complex mixtures and polydisperse samples as well as versatility of analytical ultracentrifugation (AUC) for colloid chemistry.
Sedimentation velocity (SV) experiments on dispersed nanoparticles present an effective means for determining the hard-to-evaluate properties of nanoparticles. This webinar focuses on leveraging the capabilities of SV methods for characterizing single-wall carbon nanotubes (SWCNTs). Results detailing the use of density contrast techniques on the characterization of the interfacial layer on specific SWCNT species demonstrate the power of analytical ultracentrifugation to resolve this interfacial layer.
A brief but informative visual demonstration of the process of analytical ultracentrifugation, which enables live monitoring of macromolecules in solution under native, biologically relevant conditions
A brief overview of some of the features of the Optima AUC analytical ultracentrifuge that make it the most robust research technology available for thorough analysis of proteins, viruses and other macromolecules in their native, matrix-free state
A brief review of highlights in the long history of analytical ultracentrifugation -- from the 1920s through the dawn of the 21st century -- focused on its importance in the fields of science, medicine and industry