Automated NEBNext Ultra Directional RNA Library Prep Kit
Webinar covers RNA nanotechnology in cancer therapy.RNA/RNA-based nanoparticle purification by ultracentrifugation including current technologies, different methods of ultracentrifugation, why large quantities of RNA are needed, methods for large-scale RNA purification.
Part 2 in three-part series covering automated NGS workflows. This session addresses automated DNA & RNA sequencing methods including whole genome sequencing and Illumina products (TrueSeq DNA PCR-Free, Nano DNA); targeted DNA sequencing including Agilent HaloPlex; the transcriptome and automated total RNA and mRNA sequencing and library construction/QC, including a results overview.
Learn how the Agencourt FormaPure kit supports successful RNA and miRNA extractions from FFPE tissue.Use FormaPure to isolate microRNA, RNA, DNA and total nucleic acid and suppor downstream applications including qPCR/q-RT-PCR, microarray and next-gen sequencing.
Michaela Bowden (CEMOP Associate Director, Dana Farber Cancer Institute) presents the utility of Biomek automation (library prep, etc.) and using biopsied/low RNA-input (FFPE) samples for clinically relevant results. Includes overview of the RNA-Seq workflow and Illumina's Script-Seq Complete, TruSeq Stranded mRNA kits.