Analytical Ultracentrifugation

In contrast to other methods used to analyze macromolecules, analytical ultracentrifugation (AUC) enables characterization of samples in their native state under biologically relevant solution conditions. AUC is the most versatile, rigorous and accurate technology available for determining the molecular weight, hydrodynamic and thermodynamic properties of a protein or other macromolecule. It can be used to investigate nearly any type of molecule or particle over a wide range of concentrations and in a diverse variety of solvents. For many research questions, there is no satisfactory analytical substitute for AUC.

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Advances in Sedimentation Analysis by Dr. Borries Demeler This presentation covers advances in sedimentation velocity (SV) analysis including methods for accurately characterizing heterogeneous systems at high resolution; approaches to extract rich information from SV experiments and techniques to measure the strength of macromolecular interactions with discrete binding models to whole boundary SV experiments in analytical ultracentrifugation.
The Use of the Analytical Ultracentrifuge to Characterize Very Large Complex Biopharmaceuticals This webinar covers the key attributes of AUC as a highly effective technique for characterizing viral vectors for viral gene therapy delivery systems
Analytical Ultracentrifugation in the Biopharmaceutical Industry Dr. Alexander Bepperling focuses on the data analysis tools to achieve high precision when analyzing biopharmaceutical products due to an interest in minor species of about 1-2% usually comprising of the total protein content. He focuses on the data analysis done in SEDFIT. Learn how to set up continuous c(S) distribution analysis as implemented with SEDFIT software for enhanced reproducibility during a study of many batches of one protein. Also covers applications of sedimentation equilibrium (SE) for screening weak interactions and determining detergent binding to proteins.
Addressing the Challenges of Purification & Quality Control in Gene Therapy Our panelists will focus on several pertinent topics in rAAV process development including current challenges, technologies for overcoming quality control hurdles, best practices for downstream processing, as well as discuss the current trends and future outlook of this rapidly growing therapeutic frontier.
Looking Under the Hood: Getting Started with the Optima AUC In the webinar we discuss the fundamentals of analytical ultracentrifugation and look at a few sample applications.