Nanoparticle Research

Leverage the power of flow cytometry for the detection and characterization of cellular origin nanoparticles

Interest in nanoparticles of cellular origin is exploding due to new insights into the role these particles play in extracellular communication processes and the potential for detecting pathological states via liquid biopsy.

Several fundamental capabilities of flow cytometry make this an attractive platform for extracellular vesicle research, such as the ability to detect large numbers of events and discriminate rare events while collecting information on surface composition.

Recent developments in laser and electronics design pushed the boundaries of reliably detecting events in the nanoparticle size range. The CytoFLEX Flow Cytometer is the first benchtop instrument with acceptable noise-to-signal discrimination for the reliable detection of particles down to 80 nm.

Nanoparticle Resources

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Title	Description
How can I differentiate between particulate in the sample, the sample probe, or the sheath solution? {C761825C-5739-42D5-90E3-D058FB587ADC}
Does the broad distribution of EV populations affect resolution from background? {C761825C-5739-42D5-90E3-D058FB587ADC}
Does ultracentrifugation processing duration correlate with successful isolation of exosomes? {C761825C-5739-42D5-90E3-D058FB587ADC}	Centrifugation processing time and successful exosome isolation.
Can I tell exosomes apart from other microvesicles? {C761825C-5739-42D5-90E3-D058FB587ADC}	Differences between exosomes and other small microvesicles.
How can nanoparticles be used for immunotherapy? {C761825C-5739-42D5-90E3-D058FB587ADC}	We examine how nanoparticles can be used for immunotherapy as well as in other research areas
Following isolation, what are the main methods for analyzing EVs? {C761825C-5739-42D5-90E3-D058FB587ADC}	Methods for extracellular vesicle analysis.
Can you label intra-extracellular vesicle markers? {C761825C-5739-42D5-90E3-D058FB587ADC}
Does this tech apply for follicular fluid samples? {C761825C-5739-42D5-90E3-D058FB587ADC}
If flow rate is high, and we are studying minimal concentration particles, what should we do to detect it? {C761825C-5739-42D5-90E3-D058FB587ADC}
CytoFLEX Instrument Set-up for Measuring Extracellular Vesicles Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the instrument set up and verification steps required for measuring extracellular vesicles by flow cytometry. Instructions for reducing background particles found in buffers and reagents to enhance small particle detection.
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase
Density Gradient Separation of Gold Nanorods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
Tech Note: Violet Side Scatter Use in Nanoparticle Detection {749D68FB-4893-4B2D-BD6C-C662F0520F64}	How to Use Violet Side Scatter to Detect Nanoparticles on the CytoFLEX Flow Cytometer
Characterizing RNA Nanoparticles by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad T. Schwartz, Daniel Jasinski, and Peixuan Guo - Beckman Coulter, Inc., Indianapolis, IN \| University of Kentucky, Department of Pharmaceutical Sciences
How to use VSSC on CytoFLEX Technical Note {749D68FB-4893-4B2D-BD6C-C662F0520F64}	The purpose of this paper is to demonstrate how to setup the CytoFLEX flow cytometer to detect small particles by Violet Side Scatter (VSSC). A bead mixture is defined to assist in demonstrating the detection capabilities. Using VSSC, the CytoFLEX was able to clearly discriminate 80 nm polystyrene and 150 nm silica nanoparticles from background noise.
Accurate, Rapid Sizing of C60 Fullerene Particles {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joshua T. Robinson \| Published 2016
Twenty-seven Markers on a 3-laser Flow Cytometer {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	This poster was presented at Cyto in 2018 and demonstrates a technique to maximize the usage of channels on a flow cytometer. The method relies on stacking biomarkers that are not expressed on the same cell and a gating technique to interrogate the populations of interest.
A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	In this poster, we will demonstrate the VSSC-based size resolution and fluorescence sensitivity of our prototype using a variety of NIST-traceable size standards and fluorescent nanoparticles
Characterizing the Light-Scatter Sensitivity of the CytoFLEX Flow Cytometer {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	The CytoFLEX is so sensitive that the SSC detector actually has an attenuation filter to reduce 95% of the scatter signal, adjusting it to a range useful for cells
Using Violet Side Scatter to Detect and Resolve Nanoparticles on the CytoFLEX Flow Cytometer {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	This poster provides detailed instructions for setting up the CytoFLEX flow cytometer Violet Side Scatter for nanoparticle detection. Experimental considerations for nanoscale flow cytometry and illustrated with data from the instrument.
Resolving Dyes with Identical Emission Wavelengths on the CytoFLEX {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Compensation in flow cytometry is a special case of spectral deconvolution. In this poster we demonstrate that the CytoFLEX can deconvolute highly overlapping fluorophores using the compensation algorithm.
Nano- and Microparticle Bead Mix for Use as a Sizing Standard in Flow Cytometry {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	The CytoFLEX platform is distinguished for not only its fluorescence sensitivity but also its ability to detect in the nanoparticle range using Violet Side Scatter. This poster, presented at Cyto in 2016 describes a NIST-traceable bead mix for use in standardizing flow cytometry experiments.
Preparing a CytoFLEX for Nanoscale Flow Cytometry {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	In this poster, we will demonstrate how to properly setup and clean a CytoFLEX flow cytometer for NP analyses
What guidelines are available for working with EVs? {C761825C-5739-42D5-90E3-D058FB587ADC}
What benefit can analyzing EVs by flow Cytometry give, over more traditional methods like western blotting? {C761825C-5739-42D5-90E3-D058FB587ADC}
CytoFLEX Flow Cytometer Platform Join the Resolution Revolution {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	The CytoFLEX Platform is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity
MoFlo Astrios EQ {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Small Particle FSC Enhancement Module \| Six-way sort purity > 99 % Purity \| Integrated Bio Safety Hood: Baker SterilGARD II BSL2 Cabinet
MoFlo Astrios EQ Series {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Resolution: Resolves minimum 0.2μm diameter particles in forward scatter from background
Analytical Ultracentrifugation in Nanoparticle Analysis	Joshua Robinson covers the utility of fractionation in the investigation of complex mixtures and polydisperse samples as well as versatility of analytical ultracentrifugation (AUC) for colloid chemistry. He quickly reviews analytical ultracentrifugation (AUC) theory, then discusses deconstructing nano-constructs and how AUC has been used in recent literature to really probe the underlying structure of nano-constructs, nanomaterials. Other topics covered include nanoparticle sample preparation, as well as Beckman Coulter's research and future perspectives.
Verifying for Small Particle with Certified Beads {C761825C-5739-42D5-90E3-D058FB587ADC}
Western Blot and Other Methods vs. Nanoscale Flow Cytometry {C761825C-5739-42D5-90E3-D058FB587ADC}
The Importance of Guidelines in Small Particle Research {C761825C-5739-42D5-90E3-D058FB587ADC}
Browse Nanoscale FAQs	Here is an aggregated collection of all FAQs relating to Biological Nanoparticle Analysis and Research
Small Particle {611E05FD-FC41-4E23-9498-DA5662F8D4F9}	We explore research of small biological particles, including extracellular vesicles, algae, viruses, bacteria, and more.
Physical and Chemical Characterization of Nanoparticle Constructs Using the Analytical Ultracentrifuge	Webinar covers physical and chemical characterization of nanoparticle constructs using analytical ultracentrifugation. Webinar is presented by Osman M Bakr from King Abdullah University of Science and Technology.
Quantitative Determination of Reaction Stoichiometry, Interaction Energies, and Solute Masses Using Analytical Ultracentrifugation	Live broadcast covering applications of analytical ultracentrifugation (AUC).
Large Scale Purification of RNA and RNA-based Nanoparticles by Preparative Ultracentrifugation	Webinar from Daniel Jasinski covers RNA nanotechnology in cancer therapy. RNA/RNA-based nanoparticle purification by ultracentrifugation including current technologies, different methods of ultracentrifugation, why large quantities of RNA are needed, methods for large-scale RNA purification.
Looking Under the Hood: Getting Started with the Optima AUC	n the webinar we discussed the fundamentals of analytical ultracentrifugation and looked at a few sample applications. <h3></h3>
Webinar: An Intro to Characterization of Biomolecules Using AUC	<span style="background-color: #ffffff; color: #5b5b5b;">Analytical Ultracentrifugation (AUC) is one of the most powerful biophysical tools used today for the characterization of biological samples ranging from small drug molecules to intact viruses, vesicles and microparticles</span>
Dr. Karla Williams on Analyzing Extracellular Vesicles Using the CytoFLEX
Extracellular Vesicles for ‘Liquid Biopsy’ Development in Cancer by Dr. Karla Williams	Using nano-scale flow cytometry, the Williams lab has identified a secondary bio-marker that allows them to identify the most critical prostrate cancer in patient samples. This revolutionary discovery has the potential to reduce unnecessary needle biopsy surgeries because only a blood sample is needed, as well as reduce overall costs to test for the disease. The Williams lab expects to move to clinical trial phase in the coming months.
Pushing the Boundaries of Nanoparticle Characterization	Simultaneous dynamic light scattering and zeta potential. Using carbon nanotubes in Cancer therapy and characterization of nanotubes. Beckman Coulter Life Sciences presents a video on pushing the boundaries of nanoparticle characterization with ultra-fast, simultaneous dynamic light scattering and zeta potential.
Will Violet Side-Scatter improve detection on any flow cytometers? {C761825C-5739-42D5-90E3-D058FB587ADC}
Nanoparticle Research	Why has interest in nanoparticles of cellular origin expanded so rapidly? Because of the role they play in extracellular communication processes, and their potential for detecting pathological states; learn more here
Grading of nanocellulose using a centrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}

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Nanoparticle Research

Leverage the power of flow cytometry for the detection and characterization of cellular origin nanoparticles

Nanoparticle Resources

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