Application Notes

The real-world benefits provided by our technologies are realized only through their application.

Our growing repository of application notes runs the gamut of topics—from cutting-edge research on genomic and biopharmaceutical therapies to total organic carbon analysis for modern water systems.

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Title	Description
CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
The Particle Analyzers Advancing Life Science Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Scientists share how automated cell counting is revolutionizing research
AGENCOURT® FormaPure®AGENCOURT® FormaPure® for miRNA and total RNA for miRNA and total RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter Life Sciences
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt Genfind v2 system, genomic DNA isolation kit.
Agencourt RNAdvance Blood Kit for Free Circulating DNA and miRNA/RNA Isolation from 200-300μL of Plasma and Serum {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the Illumina TruSight® HLA Sequencing Panel on the Beckman Coulter Biomek FXP HLA SP.
Improved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate an improved automated sample preparation for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis of 25-OH-Vitamin D3.
Automated Sample Preparation for the Monitoring of Ethanol Metabolites in Urine by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we describe an LC-MS/MS method for the analysis of EtG and EtS in urine employing a simple, automated sample preparation methodology using a liquid handling-based automation system.
Real Time Determination Of Cell Volume Changes Of Sperm Used For Cryopreservation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Much of the cellular damage produced by freezing is due to freezing-induced dehydration and thawing-induced rehydration.
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters.
Highly-efficient miRNA Isolation using the Agencourt FormaPure and RNAdvance Cell v2 Kits and Biomek Automated Extraction Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we describe an efficient and robust miRNA extraction method using Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation.
Automated TruSeq RNA Sample Preparation from FFPE tissue specimens utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes automated RNA library construction using the Beckman Coulter Biomek FXP Liquid Handler from archival FFPE tissue specimens.
Automated Size Selection using SPRIselect beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes the automation of the Size Selection using SPRIselect beads on the Biomek FX Liquid Handler.
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D Lund, Beckman Coulter Life Sciences
Automation of Micro RNA and Total RNA Purification from Plasma Using the Agencourt RNAdvance Blood Kits and Biomek Span-8 Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee, Ph.D. \| John Palys, Beckman Coulter Life Sciences
Automated Sample Preparation for Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The automation of sample preparation and analysis workflows for a variety of common cellular assays.
Automation of NGS Sample Size Selection using SPRIselect {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To streamline the NGS sample preparation process, Beckman Coulter has recently launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample preparations.
Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/ MS and applied to the monitoring of multiple classes of pharmaceutical and illicit drugs.
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Three-dimensional (3D) cell cultures offer greater physiological relevance than monolayer cultures for cellular interaction studies and compound screens.
Automated Sample Preparation for Analysis of 25-OH Vitamin D3 and 25-OH-Vitamin D2 by LC/MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work, we demonstrate the feasibility of automating the sample preparation for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis of 25-OH-Vitamin D2 and 25-OH-Vitamin D3.
Automated XTT Assay for Cell Viability Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cellular viability assays are valuable for numerous workflows—from establishing proliferation rates to drug toxicity screens.
Illumina TruSeq Exome Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
A Fully-Automated, Image-Based Screen for miRNAs that Regulate Cancer Cell Viability and Chemosensitivity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist, \| Beckman Coulter Life Sciences, Indianapolis, IN \| Tammy Chan, Field Application Scientist, \| Molecular Devices, Sunnyvale, CA \| Kristin Prasauckas, Manager, Field Applications Scientist, \| Molecular Devices, Sunnyvale, CA \|Tara Jones-Roe, Marketing Manager, \| Beckman Coulter Life Sciences, Indianapolis, IN
FormaPure DNA FFPE Extraction Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Increasingly, Formalin-Fixed, Paraffin-Embedded (FFPE) samples are an essential part of many next-generation sequencing (NGS) sample prep workflows, specifically in the field of cancer research
Agencourt RNAdvance Cell v2 System - Total RNA Isolation and Purification from Cultured Eukaryotic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Cell v2 kit is based on patented Solid Phase Reversible Immobilization (SPRIA) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield
LS13320XR Laser Diffraction Particle Size Distribution Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample Preparation – How to measure success!
Supplemental Protocol for miRNA Enrichment using SPRIselect {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Agencourt RNAdvance Blood Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Supplemental protocol for micro RNA and total RNA isolation from PAXgene preserved blood.
Solid Phase Reverse Immobilization (SPRI) Bead Technology for Micro RNA Clean Up using the Agencourt RNAClean XP Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Automated FFPE DNA Extraction using the FormaPure DNA Extraction Kit using the Biomek Series of Automated Liquid Handlers from Beckman Coulter, Inc. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-occurring Regulatory T-cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Carlos A. Garcia Santana M.D., Ph.D. \| James W. Tung Ph.D. \| Sergei Gulnik Ph.D.
Advancing Science is in our DNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	From the Human Genome Project to today’s quest for precision medicine, we’ve long invested in providing genomic products that reduce complexity and improve productivity
MicroRNA and RNA Extraction from Plasma and Serum using Beckman Coulter’s Agencourt RNAdvance Blood Kits {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D. Staff Application Scientist, Beckman Coulter Life Sciences \| Published 2015
Secondary Screening Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, our series of Biomek automated workstations provide comprehensive tailored solutions for your high throughput screening workflows
Automation of RNA Isolation from Blood Samples stabilized in RNAgard® Blood Tubes, using the Magnetic Bead-Based Agencourt RNAdvance Blood System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Clinical Research studies often require blood sample collection at multiple geographic sites under a wide range of conditions
Automated DNA Library Construction using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, M.S. \| Beckman Coulter, Inc. Daniela Munafo, Ph.D. \| New England Biolabs, Inc. Brad Langhorst, Ph.D. \| New England Biolabs, Inc. Laurie Mazzola \| New England Biolabs, Inc. Joanna Bybee \| New England Biolabs, Inc. Danielle Rivizzigno \| New England Biolabs, Inc. \| Published 2016
Highly Efficient Micro RNA Enrichment Procedure using Solid Phase Reverse Immobilization Magnetic Bead Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee,Ph.D. Sr. Application Scientist, Beckman Coulter Life Sciences \| Published 2013
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN USA \| Published 2014
Simple, Yet Effective – Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Excellent consistency of automated standard dilution and Bradford assay preparation
Automated Illumina TruSeq Custom Amplicon Low Input Library Preparation Kit on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
Quantification of Cellular Toxicity with Particle Characterization and Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles, reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.
Automated ELISAs—Save Time and Increase Throughput {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate how the integration of the MultiWash™+ Microplate Washer and EMax® Plus Microplate Reader to a Biomek NXP Workstation facilitates the automated processing and analysis of ELISAs.
Automating Bradford Assays—Reliable Results with Less Effort {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work we demonstrate the automated preparation and analysis of Bradford protein assay samples using a Biomek NXP Workstation with an integrated EMax® Plus Microplate Reader.
Agencourt DNAdvance System Nucleic Acid Extraction from Mammalian Tissue Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Built on SPRIB paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Automation of Multi-Day Cellular Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate the use of SAMI Process Management software to schedule and execute the automated liquid handling steps on a Biomek SAMI EX Workstation, for overlapping 8-day embryonic stem cell differentiation experiments.
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we describe a variety of automated solutions to reduce the time, effort, and complexity associated with sample preparation workflows for cell imaging.
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S.
Automated Illumina TruSeq Nano DNA Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. - Beckman Coulter, Inc. \| Published 2016
Reproducible and Efficient Automation of DTR Purification Using the Biomek 4000 Workstation and CleanSEQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CleanSEQ* application for the Biomek 4000 Laboratory Automation Workstation is a rapid, high performance dye-terminator removal method utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic beadbased technology.
Reproducible and Efficient Automation of DTR Purification Using the Biomek 4000 Workstation and CleanSEQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Alexandra Scavo - Senior Applications Scientist, John Palys - Systems Engineer II, Beckman Coulter Life Sciences, Danvers, Mass., USA
Biomek Automated 384-well qPCR Setup using KAPA Biosystems Library Quantification Kit for Illumina Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Library quantification is an essential step in the illumina next-generation sequencing(NGS) workflow
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2016
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The manual preparation of large numbers of sequencing libraries, which can be labor-intensive and time-consuming, is a bottleneck for many laboratories
High Recovery of Nucleic Acids withSuperior Yield and Purity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt DNAdvance system, nucleic acid extraction from mammalian tissue samples.
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years.
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D. Lund, Beckman Coulter Life Sciences, Indianapolis, IN \| Published: 2016
MicroRNA Extraction from Purified Exosomes Using Beckman’s Agencourt RNAdvance Cell v2 Kits and the Biomek NXP MC Platform {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee, Randy Carney, John Palys, Sidhartha Hazari, Alisha Knudson, and Kit S. Lam *University of California, Davis and Beckman Coulter Life Sciences \| Published 2015
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate total RNA (microRNA and mRNA).
Illumina TruSeq Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded mRNA Sample Preparation Kit protocol converts mRNA from total RNA into a template sample of known origin
Particle Characterization - Nanotube Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carbon nanotubes are an emerging material that has had increasing industrial application in material reinforcement and great future potential in electronics, energy storage and conversion, and medicine
MoFlo™ Bacterial Proliferation and PHB Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Population dynamics were follow ed during growth on phenol in response to adaptation processes of Cupriavidus necato, bacterial individuals morphologically nearly uniform, yet structurally and functionally diverse
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc., Indianapolis, IN \| Published 2011
MoFlo™ Fluorescence Resonance Energy Transfer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Fluorescent proteins have become a mainstay in the study of protein localization, protein-protein interaction and gene expression
MoFlo™ Chromosome Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometric chromosome classification (flow karyotyping) has been routinely used in the analysis of mammalian karyotypes as well as to assess chromosomal abnormalities
PerFix-nc (No Centrifuge Assay): A New Intracellular No-Wash Assay for Whole Blood Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Fabrice Malergue, Valerie Mallet, Laeticia Khemici, Caroline Scifo, Sylvain Monseaux, Tewfik Miloud Emmanuel Gautherot, Felix A. Montero-Julian \| Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France
Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Yang, Samantha Bauchiero, James W. Tung, Valerie Mallet, Lidice Lopez Vincent Shankey, Emmanuel Gautherot \| Beckman Coulter Inc, Miami, FL, USA and Marseille, France
Dendritic Cells Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Main Dendritic cells markers
A Method for Lysing Red Blood Cells In Vi-CELL Sample Preparations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Blood cell viability measurements on the Beckman Coulter Vi-CELL will almost entirely involve analyses of the White Blood Cell population
MoFlo Astrios EQ Enhanced Forward Scatter: Simultaneous Sorting of Micro- and Macro- Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The MoFlo Astrios EQ series provides solutions to investigate biological materials between 200 nm and 30 μm
High Purity Simultaneous Cell Sorting of Nano and Large Phytoplankton Using the MoFlo Astrios EQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Phytoplankton conversion of light on the upper limits of the ocean consists of half of the photosynthesis on the Earth
Background Subtraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	When measuring a water sample to quantify TOC (Total Organic Carbon), most TOC analyzers add some combination of reagents and dilution water to the initial sample in order to complete the measurement
Issues with Testing Jet Fuels for Contamination! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joe Dabbs \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
The Coulter Principle for Outlier Detection in Highly Concentrated Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Particle characterization techniques generally fall into one of two categories: “ensemble” techniques (multiple par ticles are analyzed simultaneously), or “discrete” techniques (individual particles are measured one at a time)
Inorganic Carbon Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
Techniques of the Coulter Principle and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Liposomes are special lipid vesicles ranging from 50 nm to 5 μm in diameter
Using Laser Diffraction Analysis in Pigment Sizing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Pigments and paints are an important class of industrial materials
8011+ Web Server Application Setup Procedure {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Contamination Monitoring in the Hydraulics Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Calibration {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The calibration of a Total Organic Carbon (TOC) Analyzer is a crucial part of ensuring a quality measurement
Sieves to Laser Diffraction...Hours to Seconds. See What You've Been Missing! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Published 2013
Cell Separation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	During various stages of the cell culturing process, centrifugation is frequently used to isolate extracellular products or to separate cells from their aqueous environment
Optimizing Cell Separation with Beckman Coulter's Centrifugal Elutriation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In many applications of cell biology, separation of cells from a heterogenous population to an enriched population of specific target cells is necessary to answer relevant scientific questions.
Density Gradient Separation of Gold Nanorods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
Analysis of Membrane Protein Dimerization by Sedimentation Equilibrium {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Karen G. Fleming - Department of Molecular Biophysics and Biochemistry, New Haven, CT
Chymotrypsin: Characterization of a Self-Associating System in the Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Paul Voelker and Don McRorie \| Beckman Coulter
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years
Exosome-Depleted FBS Using Beckman Coulter Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D. \| Beckman Coulter Life Sciences, Indianapolis, IN
Efficient Kit-Free Nucleic Acid Isolation uses a Combination of Precipitation and Centrifugation Separation Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Julia P Luciano-Chadee, Beckman Coulter, Inc., Indianapolis, IN 46268 \| Published 2017
Get Control in GMP Environments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Floor-standing ultracentrifuges and high-performance centrifuges are vital to many bioproduction processes
Rapid Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: R. A. Gray, A. Stern, T. Bewley, and S. J. Shire Genentech, Inc. \| South San Francisco, CA
A Standardized, Automated Approach for Exosome Isolation and Characterization using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D., Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN 46268
Lentiviral Vector Preparation Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells
Enhancing Vaccine Development and Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Senior Application Scientist \| Beckman Coulter, Inc., Life Sciences, Indianapolis, IN USA \| Published 2015
Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Thomas M. Laue University of New Hampshire Department of Biochemistry Durham, NH 03824
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Application Scientist \| Beckman Coulter, Inc., Indianapolis, IN USA \| Published 2014
Avanti JXN Series - Complete Solution for Protein Purification Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays
High-Performance Automation and Centrifugation Preparation of Carbon Nanotubes for Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Single-Walled Carbon Nanotubes (SWCNT) experienced increasing interest in the past 15 years for semiconductors, fuel cells, and biomedical applications
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph.D., Dean Clodfelter, M.S. \| Beckman Coulter, Inc., Indianapolis, IN 46268
Beckman Coulter’s New Line of Centrifuges Provides Support for 21 CFR Part 11 Compliant Laboratories {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As technology and software advance, security and protection of data is becoming increasingly important to ensure a properly functioning lab environment
The Lipemic Serum Clarification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Lipemic Serum Clarifi cation System clears blood samples of lipemia caused by chylomicrons—fat particles 80–500 nm in diameter that impair accuracy of spectrophotometric analyses
Linearity of BSA Using Absorbance & Interference Optics {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dean Clodfelter, M.S., \|Chad Schwartz, Ph.D., Beckman Coulter Inc., 5350 Lakeview Parkway Dr., Indianapolis, IN 46268
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase
Stability of the Human Immunodeficiency Virus-1 Reverse Transcriptase Heterodimer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jacob Lebowitz - University of Alabama, Birmingham, AL
The Solution Conformation of Novel Antibody Fragments Studied Using the Proteomelab XL –A Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Peter J. Morgan, Olwyn D. Byron, Stephen E. Harding \| Department of Applied Biochemistry and Food Science \| University of Nottingham, Sutton Bonington, U. K.
The Sizing of Non-Spherical, Sub-Micron Particles using Polarization Intensity Differential Scattering (PIDS™) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nearly all laser-based particle-sizing devices make no allowance for the shape of the materials under test, regardless of the size of the particles
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase.
How to Characterize Multimodal Samples using Particle Characterization Equipment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note will discuss the topic of size resolution in particle characterization and advantages and disadvantages of various techniques
Particle Characterization - Flow Pressurization Eliminates Bubbles for More Accuracy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Bubbles create major issues when characterizing nanoparticles in solution because the characterization techniques typically utilize light scattering specifically when determining size distribution and stability
Achieving Compliant Batch Release - Sterile Parenteral Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Tony Harrison, Compliance and Applications Specialist \| Beckman Coulter, Inc., United Kingdom \| Published 2016
Method Overview {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
Circulating Cell Free DNA And MiRNA Extraction From Plasma Using Beckman Coulter’s Agencourt RNAdvance Blood Kits {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Using The Agencourt ® AMPure® And Agencourt CleanSEQ® Methods To Manage A Variety Of Input Sample Quality {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we present sample purification methods that assist in addressing the varying input sample quality encountered by facilities performing PCR amplification and sequencing for SNP detection.
The MD Anderson SPRI8 Experience: Using Agencourt8 CleanSEQ8 and Agencourt AMPure8 in HLA Sequencing-Based Typing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Cano, Pedro \| Fernandez-Vina, Marcelo \| Guerrero, Edward \| Barnes, Titus \| Partlow, David \| Willis, Dana \| Zhao, Weicheng \| Patel, Hemantkumar \| Heins-Israel, Karen \| EL-Fahmawi, Bassam
Mo Bio PowerMag Soil DNA Isolation Kit on Biomek 4000 Liquid Handling Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Shilpa Parakh, Beckman Coulter, Inc \| Published 2016
Solid Phase Reverse Immobilization (SPRI) Bead Technology for Micro RNA Clean Up using the Agencourt RNAClean XP Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D. Staff Application Scientist, Beckman Coulter Life Sciences \| Published 2014
Agencourt® FormaPure® for miRNA and Total RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This protocol describes the procedure for purifying miRNA, including total RNA, from formalin-fixed, paraffin embedded (FFPE) tissue sections
Continuous Cell Culture {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Continuous cell culture provides scientists the ability to readily have assay-ready plates available to test potential agents or to have large quantities of multiple cell lines available for therapies research.
Micro RNA and Total RNA Purification from Tissues using the AgencourtRNAdvance Tissue Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D. Staff Application Scientist, Beckman Coulter Life Sciences \| Published 2014
FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The FormaPure DNA extraction kit provides a convenient extraction process utilizing Beckman Coulter’s proprietary solid phase reversible immobilization (SPRI) chemistry to extract DNA from FFPE curls.
Reproducible and Efficient Automation of PCR Reaction Setup and Purification Using the Biomek 4000 Workstation and AMPure XP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ruth Zhang, Li Liu, Amy Yoder, John Palys, Bryan Daniels, Isabel Gautreau and William Godfrey Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2013
Automation of NGS Sample Size Selection using SPRIselect on the Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ruth Zhang, Sr. Development Scientist, Li Liu, Sr. Development Scientist, Mary Blair, Sr. Applications Scientist, Amy Yoder, Staff Development Scientist, Alexandra Scavo, Sr. Applications Scientist, and William Godfrey, Sr. Manager Development Science, Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2013
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood and serum
Automated Solutions for Cellular Analysis Through Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski - Staff Applications Scientist, Li Liu - Senior Development Scientist, Amy Yoder - Staff Development Scientist, Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2014
Automation of Cell Staining Using the Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Manual cell staining of cardiomyocytes, either to characterize antigen expression or to assess cell health, is labor-intensive and time-consuming.
Automated Data Management for Reliable Cell-Based Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN Timothy Sherrill, Staff Systems Engineer \| Beckman Coulter Life Sciences, Indianapolis, IN Tara Jones-Roe, Marketing Manager \| Beckman Coulter Life Sciences, Indianapolis, IN
High Quality RNA from Preserved Blood {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt RNAdvance Blood System, RNA extraction from RNA-stabilizing blood collection tubes.
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Demonstrated application on how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Flexible ELISA Automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automated ELISA assay workflow \| Optimized liquid transfers eliminate the need for a plate washer \| High-capacity deck accommodates fresh tip usage
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
AGENCOURT® RNAdvance® CELL v2 Supplemental Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For micro RNA and total RNA isolation from cultured cells.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, MS, Senior Applications Scientist - Beckman Coulter, Inc. \| Nate Baird, PhD, Scientist, Illumina \| Brad Baas, PhD, Senior Scientist, Illumina \| Published 2015
Automated Salt-Assisted Liquid-Liquid Extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski and Ruth Zhang \| Beckman Coulter Life Sciences, Indianapolis, IN, USA, Yun Yun Zou and Michael Jarvis \| SCIEX, Concord, ON, Canada
Agencourt RNAClean XP System - Enzymatic Purification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions
Automated Optimization of IgG Production in CHO Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist - Beckman Coulter Life Sciences, Indianapolis, IN \| David Apiyo, Marketing Applications Manager - Pall ForteBio, Fremont, CA \| Rashi Takkar, Product Manager - Pall ForteBio, Fremont, CA \| Tara Jones-Roe, Marketing Manager -Beckman Coulter Life Sciences, Indianapolis, IN
Stem Cell Analyses Using The Vi-CELL™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.
Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext Ultra DNA Library Preparation Kit for Illumina on the Beckman Coulter Biomek FXP Dual-Arm Multichannel 96 and Span-8 automated liquid handler (Biomek FXP ).
Highly Efficient Micro RNA Enrichment Procedure using Solid Phase Reverse Immobilization Magnetic Bead Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Scalable, Automation-friendly and High Throughput PCR Purification {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ system, dye terminator removal.
Extraction and Purification of High Quality RNA from Many Tissue Types {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt RNAdvance tissue system, total RNA extraction from tissue
The New Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	By focusing on sample protection, the Avanti J-15 series has reduced sample disturbance during acceleration and deceleration, increasing sample yield, decreasing contamination and increasing centrifugation efficiency.
High-Capacity Next Generation Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation
The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The implementation of Ultra Harmonic Technology into the Avanti J-15 series renders our centrifuge ideal for applications that require multiple runs. It saves up to 10% of instrument usage time for a single application and the time savings maximizes overall laboratory assent management.
Met One 3400 Series Portable Airborne Particle Counter {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The new enhanced MET ONE 3400 Series is the easiest-to-use and easiest-to-integrate portable particle counter for any facility or environmental monitoring program
Enumeration and Size Distribution of Yeast Cells in the Brewing Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Steve Szabo, Ken Lindo, Brendan Yee \| Cellular Analysis Business Center, Beckman Coulter Inc, Miami, FL
Determination of Size and Concentration of Particles in Oils {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in an oil sample may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Evaluating Contact Lens Disinfection Efficacy Using the Beckman Coulter™ Multisizer™ 3 COULTER COUNTER® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The percentage of aggregates in bacteria cultures may be determined by employing the Coulter Principle, also known as Electrical Sensing Zone (ESZ) technique
Beer, Evaluation of Final Product and Filtration Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Using Pressure to Reduce Bubble Contamination from Particle Count Results {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc, Grants Pass, OR \| Published 2016
Using Laser Diffraction in Folk and Ward Phi Notation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Soil studies span a range of applications including agriculture, architectural planning, and historical environmental studies
Using the DelsaMax CORE for Molecular Weight Analysis - Protein Sizing Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Accurate determination of protein and antibody molecular weight has great impor tance in the field of molecular biology
USP System Suitability {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The US Pharmacopeia (USP 36-NF 31, section <643>) provides specific guidance on how to qualify TOC instrumentation for use
Coulter Principle Short Course {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	What is a particle? According to Webster’s Dictionary a particle is “a minute quantity or fragment” or “a relatively small or the smallest discrete portion or amount of something
HIAC 8011+ Using Pressure To Reduce Entrained Air from Fluid Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Global Marketing Manager for Beckman Coulter Life Sciences \| Published 2013
Industrial Application Note 8011+ Reporting Standards Feature and Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Determine Hydrodynamic Size of Surfactant Micelles with DelsaMax CORE {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sodium Dodecyl Sulfate (SDS) is a commonly used amphiphilic surfactant with wide-ranging applications, including chemical disinfection to protein denaturation in SDS-PAGE gels, cell lysing for DNA extraction, and nanoparticle solubilization
Biological Applications for Multisizer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Aspirin Analogues and Flow of Erythrocytes through Narrow Capillaries
Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Sr. Application Scientist for Beckman Coulter Life Sciences in Grants Pass, Oregon, USA \| Published 2013
Minimal Sample to Sample Carry Over with the Newly Designed HIAC 8011+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Engineer / Scientist for Beckman Coulter Life Sciences in Grants Pass, Oregon, USA. \| Published 2013
ISO11171-2010 Standard Requirement Changes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Applications Scientist for Beckman Coulter, Inc. in Grants Pass, Oregon, USA
Automating 3-D Cell Culture and Screening by Flow Cytometry and High-Content Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Michael Kowalski, Ph.D. \| Staff applications scientist for Beckman Coulter Life Sciences Indianapolis, IN \| Published 2016
Enzymatic Determination Of Glycerol Contents In Biological Samples Using The Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Jörg Mampel, B.R.A.I.N Aktiengesellschaft, Darmstaedter Str. 34-36, D-64673 Zwingenberg \| Published 2014
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith and Scott D. Michaels - The Center for Genomics and Bioinformatics - Indiana University \| Mary Blair - Life Science Division Beckman Coulter - Indianapolis, IN \| Published 2013
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Highly Efficient MicroRNA and Total RNA Purification from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee,Ph.D. Staff Application Scientist, Beckman Coulter Life Sciences \| Published 2014
Challenges in Next Gen Sequencing and How to Overcome Them {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The exponential acceleration in our capacity to sequence 3.2 billion human base pairs has transformed our approach to therapeutics, particularly for cancer.
Cell Culture Automating Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is an essential workflow that enables studies to be performed in drug discovery and scientific
Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell Culture is an essential workflow that enables studies to be performed in drug discovery and scientific research
Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As biologics research continues to evolve and gain prominence in therapy development for disease states, the need for reliable and robust cell lines will greatly increase
Circulating Cell Free DNA and miRNA Extraction from Plasma Using Beckman Coulter’s Agencourt RNAdavance Blood Kits {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D. Staff Application Scientist, Beckman Coulter Life Sciences
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter, Inc. \| Published 2014
Fully-Automated Cellular Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Direct integration of CytoFLEX Flow Cytometer to Biomek i-Series Automated Workstations enables complete automation of sample processing and data acquisition
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Application Scientist - Beckman Coulter, Inc., Indianapolis, IN USA \| Published 2014
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Design of Experiment (DOE) approach can be quickly automated on the Biomek i7 Automated Workstation to identify optimal transfection conditions for a variety
Automating Biologics Signature Peptide Quantitation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ian Moore, Technical Marketing Manager \| SCIEX, Concord, Canada, Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential
Biomek FXP Laboratory Automation Workstation as a Tool for Discovering Targets & Compounds for Host-Directed Influenza Therapy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Markus Lesch, Project Manager, Center for Systems Biomedicine, Steinbeis Innovation gGmbH. Falkensee, Germany, Alexander Karlas, Senior Scientist, Max Planck Institute for Infection Biology, Berlin, Germany. Published: 2015
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We show here how one can take advantage of the in Sorét band of porphyrins complexed with different cations to spectrally deconvolute dissimilar optical properties from different molecules.
High Throughput Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, screening campaigns are employed to identify potential new treatments for diseases and to answer questions that remain unknown in the scientific community.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers superior quality DNA with no salt carryover
Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The introduction of exogenous nucleic acids into cells is an essential means of studying cell biology.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers superior quality DNA with no salt carryover
Biomek i-Series1 Automated FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential for many cancer research applications
Illumina TruSeq Nano DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq Nano DNA Library Prep Kit enables preparation of uniquely indexed DNA libraries for single end and paired end sequencing
Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Improve Sample Quality with Standardized Sample Prep \| Quick Implementation with Demonstrated Methods \| Increase Throughput to Maximize Sequencing Runs
Automation of Micro RNA and Total RNA Purification from Plasma Using the Agencourt RNAdvance Blood Kits and Biomek Span-8 Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee, Ph.D. and John Palys, Beckman Coulter Life Sciences \| Published 2015
Automated TruSeq RNA Sample Preparation from FFPE Tissue Specimens Utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee Ph.D., Senior Application Scientist, and Sarah Simons, Advanced Specialist- Field Systems Support, Beckman Coulter Life Sciences \| Michaela Bowden Ph.D., Research Scientist \| Ashley Rossi, Molecular Technician, and Ed Stack Ph.D., Associate Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI \| Svitlana Tyekucheva, Department of Biostatistics, DFCI Biostatistics & Computational Biology and Massimo Loda MD., Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI and Department of Pathology at Brigham and Women's Hospital
Automated Size Selection using SPRIselect Beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Alexandre Bélisle, Vicky Arsenault, Belisle Tir, Alfredo Staffa Sylvie LaBoissière and Alexandre Montpetit - McGill University and Génome Québec Innovation Centre - Montréal, Québec, Canada
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Li Liu, Sr. Development Scientist \| Amy Yoder, Staff Development Scientist \| Beckman Coulter Life Sciences Indianapolis, IN
Full Automation of the SISCAPA® Workflow Using a Biomek NXP Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Morty Razavi, Michael Kowalski, Tara Jones-Roe, Christie Hunter, Leigh Anderson, Richard Yip, Matt Pope, Terry Pearson SISCAPA Assay Technologies, Washington DC
Workflow Questions Answered: Challenges with FFPE Tissue Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jung Doh, Ph.D., Senior Applications Scientist, Beckman Coulter, Inc. \| Published 2017
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: S. E. SZABO, S. L. MONROE, S. FIORINO, J. BITZAN, K. LOPER \| The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland; Beckman Coulter Miami, Florida, USA \| Published 2004
Agencourt® RNAdvance™ Tissue Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Supplemental protocol for micro RNA and total RNA isolation from tissue.
Highly Efficient MicroRNA and Total RNA Purification from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe how the Agencourt SPRI (Solid Phase Reverse Immobilization) magnetic bead based method is superior to the column purification method for purifying miRNA from formalin-fixed paraffin-embedded tissues (FFPE).
Agencourt RNAdvance Blood Kit for Micro RNA and Total RNA Isolation from 200μL of Plasma and Serum {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
Micro RNA and Total RNA Purification from Tissues using the AgencourtRNAdvance Tissue Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D.
AGENCOURT® FormaPure® Kit Supplemental Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For micro RNA and total RNA isolation from formalin-fixed, paraffin-embedded tissue.
Automation of Micro RNA and Total RNA Purification from Tissues Using the Agencourt RNAdvance Tissue Kits and Biomek Span-8 Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee, PhD \| John R. Palys \| Christian Croisetiere \| Isabel Gautreau
Improved Sequencing Performance with Publication-Quality Data {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CosMCPrep system is a versatile plasmid purification procedure
A Path to the Standardization of Exosome Isolation and NGS Characterization for Complex Disease Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: CHAD SCHWARTZ, PH.D. \| ZACH SMITH, M.S. \| Published October 2015
Highly-Efficient miRNA Isolation using the Agencourt FormaPure and RNAdvance Cell v2 Kits and Biomek Automated Extraction Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bee Na Lee, Ph.D. Senior Application Scientist, Beckman Coulter Life Sciences, Brea, CA, USA \| Published 2012
Agencourt RNAdvance Tissue System - Total RNA Extraction from Tissue {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue extraction technology, in combination with Beckman Coulter automation, offers researchers a true walk-away solution for consistent recovery of high quality total RNA in a multi-well format
Agencourt CleanSEQ System - Dye Terminator Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ is a SPRID (Solid Phase Reversible Immobilization) paramagnetic bead-based sequencing purification system with a simple three-step protocol
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample quality is of utmost importance to genomic researchers to better ensure good results when running laborious and expensive processes such as Next Generation Sequencing.
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith \| Scott D. Michaels \| Mary Blair
JP SDBS Validation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	JP 16 specifies that when measuring a 500ppb standard of sodium dodecylbenzenesuflonate (SDBS), a TOC analyzer should recover a value of at least 450ppb (90% recovery)
Overload Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In a typical laboratory environment, a TOC analyzer is used to measure water samples where the TOC value of those samples is unknown
Preparing Reagent Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 uses a mixture of phosphoric acid (to aid in removal of CO2 from solution) and ammonium persulfate (to aid in oxidizing organic compounds)
HIAC PODS+ Reporting Standards Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
New HIAC PODS+ …Have Internal Pump, Will Travel! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Density Gradient Separation of Gold Nanorods - Using the New Avanti JXN Centrifuge & JS-24.15 Swinging Bucket Rotor {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
HIAC PODS+ vs. Parker ACM-20 Performance Comparison {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
HIAC PODS+ Online Mode & Filter Cart Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Joe Dabbs & Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2017
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad Schwartz, Ph.D., Application Scientist I \| Randy Lockner, M.S., Centrifugation Marketing \| Randy Pawlovich, Director of Product Management & Strategy \| Beckman Coulter, Inc., Indianapolis, IN USA
Using k-Factor to Compare Rotor Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The k-factor is a common parameter that describes the efficiency of a centrifuge-rotor system, although temperature is still overlooked
ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Tony Harrison - Compliance and Applications Specialist for Beckman Coulter Life Sciences \| Published 2013
Accurate, Rapid Sizing of C60 Fullerene Particles {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joshua T. Robinson \| Published 2016
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Julia P Luciano-Chadee - Beckman Coulter, Inc., Indianapolis, IN \| Chad Schwartz Ph.D. - Beckman Coulter, Inc., Indianapolis, IN
Characterizing RNA Nanoparticles by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad T. Schwartz, Daniel Jasinski, and Peixuan Guo - Beckman Coulter, Inc., Indianapolis, IN \| University of Kentucky, Department of Pharmaceutical Sciences
Application Information Bulletin: Dendritic Cells Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Tewfik Miloud, Nathalie Dupas, Anders Kverneland, Mathias Streitz, Birgit Sawitzki and Felix A. Montero-Julian \| Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France and Institute of Medical Immunology, Charité Universitätsmedizin, Berlin, Germany
Zebra Fish Whole Kidney Marrow Cells Myeloid, Precursor, and Lymphoid % after RBC Lysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Dovey, John Tigges, Vasilis Toxavidis \| Beth Israel Deaconess Medical Center - Harvard Stem Cell Institute
Use of Beckman Coulter Reagents for Automated, Whole Blood, White Blood Cell Viability Measurements {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.\| Beckman Coulter, Inc \| Published 2004
DuraClone IM Standardized Phenotyping Panels for Studies of the Human Immune System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The continuous increase of multiplexing capability in cytometry allows to choose from an extensive menu of markers and fluorochromes
A High Recovery and High Purity Data Analysis Strategy for Rare Abnormal Plasma Cell Events using the DuraClone RE PC Tube {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr Agnieszka Blum- Charité Virchow Hospital, Stem Cell Facility, Berlin, Germany \| Dr Michael Kapinsky - Life Science, Beckman Coulter, Marseille, France
Instrument-to-Instrument Variability in the Vi-CELL Automated Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Clarence Lew, Senior Applications Scientist, John A. Gomez, Senior Staff Technical Operations Engineer, Matthew N. Rhyner, Ph.D., Global Marketing Manager-Analytical Products \| Beckman Coulter Life Sciences, Brea, CA USA \| Published 2012
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi-Cell™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S. \| Beckman Coulter, Inc. \| Published 2004
Counting Eschericia Coli Using the CytoFLEX Research Flow Cytometer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Often an enumeration of bacteria is required more quickly than a colony forming unit, CFU, assay can be completed
PerFix-nc, Procedure for PBMC (Peripheral Blood Mononuclear Cells) and Purified Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Resuspend the cells or PBMC in 100% serum (FCS or FBS), at a concentration ranging from «normal» (5.106/mL) to «high» (4.107/mL).
Automated Optimization of Murine Embryonic Stem Cell Differentiation into Cardiomyocytes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski Ph.D., Li Liu Ph.D., Amy Yoder, and Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter, Inc. Indianapolis, IN \| Published 2011
ZAP-70 Assessment by Flow Cytometry: Improving Assay Sensitivity, Robustness and Workflow using an Isoclonic Control and PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ZAP-70 testing by flow cytometry is a fast and cost-friendly alternative method to molecular assays (Crespo et al., N Engl J Med 2003)
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung \| Beckman Coulter, Inc., Miami, FL, USA
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak \| Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
6 Color Panel for Staining Human Peripheral Blood Lymphocytes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we describe immunophenotypic analysis of human peripheral blood lymphocytes utilizing a 6-color antibody panel in a stain/lyse protocol run on the CytoFLEX* Research Flow Cytometer
Enhanced Forward Scatter Module+ on the MoFlo™ Astrios: Small Particle Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Alan Dean Ph.D., Kerry Weinberg, Robin Morris, Blake McCarty and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
MoFlo™ Viability and Function with High-Speed Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
MoFlo™ Stem Cell Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified populations of functional stem cells are of great interest to the biomedical community, both in the understanding of stem cell biology and in clinical transplant settings
MoFlo™ Astrios* Enhanced Forward Scatter+: 0.2 μm to 30 μm Dynamic FSC Detection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Mike Morrell, Robin Morris, Blake McCarty, Alan Dean Ph.D. and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
Intelligent Biosafety: MoFlo Astrios Fully Integrated with the Biosafety Cabinet and Aerosol Evacuation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As with any sorter that requires jet-in-air sorting, aerosols are created both from the sort and waste streams
Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Andreas Spittler, MD, Associate Professor for Pathophysiology, Medical University of Vienna, Core Facility Flow Cytometry & Department of Surgery, Research Laboratories \| Published 2015
The Unique Peristaltic Sample Delivery System of the CytoFLEX Analyzer Enables Optimized Measurements of Transient Changes in Intracellular Calcium in Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ira Schieren, Peter Racanelli, Damian Williams \| Howard Hughes Medical Institute, Columbia University Medical Center, Beckman Coulter Inc., Miami, United States and Department of Pathology and Cell Biology, Columbia University Medical Center
Rapid and Easy Intracellular + Surface Staining of MOUSE Cells using PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Dispense 50 μL of cells into empty tube \| Add 5 μL of fixative reagent R1 (low fixation), mix tube \| Incubate 15 min. at room temperature (RT)
FoxP3 Staining in Human Whole Blood using PerFix-nc (Optimized Protocol) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Fabrice Malergue PhD (1), Carlos A. Garcia PhD (2) and Tewfik Miloud PhD (1) \| (1) Global Assay and Applications Development, Beckman Coulter Life Science Marseille France; (2) Life Science Research Beckman Coulter Life Science Miami USA
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Mary Blair
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext (Registered trademark) Ultra Directional RNA Library Prep Kit for Illumina on the Beckman Coulter Biomek FXP automated liquid handler.
Automated Cell Culture: Improving the Consistency of Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we illustrate the capabilities for automating a standard workflow for cell line maintenance and demonstrate this process using mouse embryonic stem cells.
Automated Illumina TruSeq DNA PCR-Free Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. \| Beckman Coulter, Inc.
Agencourt® RNAClean™ XP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Supplemental Protocol For Micro RNA Clean Up Using Agencourt® RNAclean® XP
Xtra Pure RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt RNAClean XP System, Enzymatic Purification System