Dynamic Light Scatter

Dynamic Light Scattering (DLS) is the most prevalent light scattering technique as it can size and characterize particles down to 1 nm diameter. With DLS, the sample is illuminated by a laser beam and a photo detector detects fluctuations in the scattered light at a known scattering angle. Particles scatter the light and as such, ‘imprint’ information about their motion – analyzing the fluctuations of the scattered light yields information about the particles themselves.

DLS is often used wherever the size distributions of macromolecules and nanoparticles must be measured quickly. Most DLS applications are in the nanometer to sub-micron size range (e.g., emulsions, micelles, polymers, proteins, nanoparticles). 

Applications include

  • Protein stability analysis
  • Formulation testing
  • Nanoparticle characterization
  • Liposome and exosome analysis
  • Polymer and macromolecule analysis
  • Aggregation detection