Centrifugal Elutriation

Optimized cell separation with the Beckman Coulter Centrifugal Elutriation System

Counter-streaming centrifugation was originated by Lindahl in 1948 and later developed by Beckman Instruments as centrifugal elutriation* (McEwenet al. 1968). This rapid separating technique is effective for purifying large quantities of similar cells and has been used to prepare a wide variety of vertebrate cells (whole blood, brain and liver cells, transformed tumor cells).

During separation, cells are suspended in a buffer solution. Any buffer and culture medium may be used with the key being the media’s capacity to maintain the physiological integrity of the cells and prevent cell aggregation.

As a result, cells separated via CE can be used for further experimentation or separation via other methods.

Elutriator Rotors

Elutriator JE 5 0 Rotor

Elutriator JE 5 0 Rotor

JE-5.0 Elutriator Rotor, Aluminum and Stainless Steel, 5,000 rpm, 4,700 x g

Major Applications: Separation of whole living cells

  • Includes bypass chamber and supplies
  • Features quick-release assembly
  • Autoclavable for sterile operation.
  • Elutriation chamber(s) ordered separately

Elutriation Chamber

Description Part NO. Max. Cells Recovered per run Minimum Cells Required for Loading Per Fraction Max Elutriated Buffer volume
Large (40 mL)  356940 1010 108 1000 mL
Standard (4 mL)  356943 109 107 250 mL
 Sanderson  356945 109 106 250 mL