FormaPure DNA Performance and Data

Genomic DNA Isolation from FFPE

Increasingly, Formalin-Fixed, Paraffin-Embedded (FFPE) samples are an essential part of many next-generation sequencing (NGS) sample prep workflows, specifically in the field of cancer research. High quality extraction protocols focused on yield and purity are essential to achieving reliable results. FormaPure DNA now provides an automation-ready, SPRI-based extraction reagent kit to support your evolving research needs. Most notably, it provides significant time savings in the form of reduced turnaround times and less than 15 minutes of hands-on time when automated on a Biomek liquid handler.

  • Minimal hands-on time
  • Dramatically increase productivity through automation - low to high throughput
  • Proven compatibility with NGS and other downstream applications
  • Consistent extraction performance
 
NEXT-GENERATION SEQUENCING COMPATIBILITY
POSITION GENE FORMAPURE DNA SAMPLES WITH VARIANT CALL (MAX 6) FORMAPURE DNA VARIANT FREQ (%) EXPECTED ALLELIC FREQ (%)
140453136 BRAF 6 12.2 10.5
55599321 KIT 6 9.2 10
55242464 EGFRΔ 6 1.8 2
55259515 EGFR 6 3.1 3
55241707 EGFR 6 25 24.5
25398281 KRAS 6 13.7 15
25398284 KRAS 6 5.3 6
115256530 NRAS 6 17.2 12.5
178952085 PIK3CA 6 17.5 17.5
178936091 PIK3CA 6 9.1 9

Table. Extraction with FormaPure DNA supports detection of low frequency variants.

 

FormaPure DNA maintains detectability of low frequency variants without introducing extraction based experimental bias. DNA extracted using FormaPure DNA is suitable for the most stringent downstream applications including Next-Generation Sequencing.

 

FormaPure DNA Average dsDNA

Average (n=8) yield per 10 μm slice. FormaPure DNA (FP DNA) compared to competitor (Q) FFPE DNA extraction kit. Multiple formalin fixed paraffin embedded (FFPE) cancer tissues were compared, including the Horizon Discovery- HD200 FFPE reference tissue.

 

FormaPure DNA Absorbance Ratio

Average (n=8) 260/280 absorbance ratios per 10 μm slice from FormaPure DNA (FP DNA) extraction method. Multiple formalin fixed paraffin embedded (FFPE) cancer tissues were evaluated, including the Horizon Discovery- HD200 FFPE reference tissue.

 

FormaPure DNA is available in three kit sizes based on your throughput needs.

PRODUCT ORDERING INFORMATION
B89230 Formapure DNA-50 Preps
B89231 Formapure DNA-96 Preps
B89232 Formapure DNA-384 Preps

 

Not intended or validated for use in the diagnosis of disease or other conditions.
Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks are the property of their respective owners.