EMnetik PCR System Performance Data

PCR Cleanup - Simplified

  • ~2x faster turnaround time compared to column cleanups (16 min vs 30 min)
  • >80% recovery (comparable to column cleanup kits)
  • Significantly fewer touchpoints (< 50 touchpoints compared to 300 for columns)
    • No need to handle small columns or use a single-channel pipette
    • Move samples from a thermocycler to the EMnetik 24 system, and don’t move them again until final elution
  • Intuitive user interface removes guesswork by providing clear, step-by-step instructions

Choose your sample input, elution and labware.

Genomics EMnetik PCR Performance Figure 1

Figure 1

These graphs demonstrate your flexible options.
Figure 1 left: Yield of elutions from 2 different starting inputs and 2 different elution volume options (50 µL and 20 µL). Bars indicate the average of 9 replicates on the device; error bars indicate the standard deviation of the replicates. Figure 1 right: Yield does not vary widely when using different labware. The first three bars show the yield using PCR 8-strip tubes, and the second three bars show the yield using single PCR tubes. The bars represent the average of 8 samples; error bars indicate the standard deviation of the 8 samples. Tubes are as follows: A: Thermo Scientific AB-2005, B:VWR 93001-118, C: VWR 20170-002, D: Thermo Scientific AB-0337, E: VWR 20170-010, and F: VWR 20170-012.

DNA is stable in the spinning magnetic beads. 

Genomics EMnetik PCR Performance Figure 2

Figure 2

Figure 2: To test that DNA was not degraded during the automatic bead mixing or separation, a ladder was used as input lanes; 1 and 2 show the input ladder and lanes 3 - 8 show the ladder after cleanup. All bands can be seen in all lanes, indicating that DNA is not degraded during the cleanup process.

EMnetik PCR Cleanup Workflow

Genomics EMnetik PCR Workflow

EMnetik PCR Cleanup Workflow

  1. Add your sample to the instrument
  2. Add EMnetik PCR cleanup kit to sample (1.8x ratio)
  3. Start bind mix and separate
  4. Remove supernatant
  5. Add ethanol to wash
  6. Remove supernatant
  7. Repeat steps 5 and 6
  8. Start ethanol dry
  9. Add eluant
  10. Start elution mix and separate
  11. Move final elution to preferred labware

 

Not intended or validated for use in the diagnosis of disease or other conditions.
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 Genomics EMnetik PCR System

EMnetik PCR Cleanup System
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EMnetik System Flyer:

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EMnetik System Overview:

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EMnetik PCR Cleanup Data Sheet:

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Genetic Engineering Timeline:

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