Instrument Setup and QC

In flow cytometry, instrument setup is required ensure consistent sample analysis. This process involves verification of optical detectors and fluidic stability, standardization of instrument detector settings and establishment of color compensation settings. Beckman Coulter offers a variety of instrument setup, fluorospheres and quality control reagent products for optimum setup, daily maintenance and operation of your Beckman Coulter flow cytometer.

  • Cell Controls
  • Compensation Kits
  • Cytometer Setup
  • Cytometer Verification

Cell Controls

AQUIOS IMMUNO-TROL Cells

AQUIOS IMMUNO-TROL Cells is an assayed, lysable whole blood quality control product for immunophenotyping analysis using monoclonal antibody reagents and flow cytometry. It provides a positive cell control that is processed in the same manner as a whole blood sample. This allows verification of instrument and reagent performance. It also verifies the methods used for staining targeted cells, the lysing of erythrocytes, and the analysis of samples by the AQUIOS CL Flow Cytometer. When using Tetra-1 Panel or Tetra-2+ Panel tests, there are 15 tests per tube. When using the Tetra Combo, there are 10 tests per tube.
 

AQUIOS IMMUNO-TROL Low Cells

AQUIOS IMMUNO-TROL Low Cells is an assayed, lysable whole blood quality control product for immunophenotyping analysis using monoclonal antibody reagents and flow cytometry. It provides a positive cell control that is processed in the same manner as a whole blood sample. This allows verification of instrument and reagent performance. It also verifies the methods used for staining targeted cells, the lysing of erythrocytes, and the analysis of samples by the AQUIOS CL Flow Cytometer.When using Tetra-1 Panel or Tetra-2+ Panel tests, there are 15 tests per tube. When using the Tetra Combo, there are 10 tests per tube.

AQUIOS IMMUNO-TROL Low Cells - PLG-Tetra

AQUIOS IMMUNO-TROL LOW Cells (PLG/Tetra) is an assayed, lysable whole blood quality control product for immunophenotyping analysis using monoclonal antibody reagents and flow cytometry. It provides a positive cell control that is processed in the same manner as a whole blood sample. This allows verification of instrument and reagent performance. It also verifies the methods used for staining targeted cells, the lysing of erythrocytes, and the analysis of samples by the AQUIOS CL Flow Cytometer.

CYTO-TROL

Lyophilized human lymphocytes used to assess activity of monoclonal antibodies. CYTO-TROL Control Cells are used to assess the activity of monoclonal antibodies by flow cytometry. They are a lyophilized preparation of human lymphocytes that exhibit surface antigens detectable with monoclonal antibodies. These cells are isolated from peripheral blood and express antigens that are representative of those found on normal lymphocytes. They may be used with directly-conjugated monoclonal antibodies.

IMMUNO-TROL Cells

IMMUNO-TROL Cells are a positive process control for flow cytometry. They are assayed for lymphocyte, granulocyte and monocyte specific antigens and single platform absolute counts. Light scatter, population distribution, fluorescence intensity, and antigen density mimic those of whole blood.

Stem-Trol

Ready-to-use highly calibrated control cells providing antibody-to-antigen positive control for absolute counting of CD34+ CD45dim cells. COULTER Stem-Trol Control Cells are preserved KG-1a cells, that have been modified and stabilized (patent pending) to express the CD34 Class III epitopes and the CD45 leucocyte common antigen at densities that approximate normal human hematopoietic progenitor cells (HPC). This product is a ready-to-use cell suspension. COULTER Stem-Trol Control Cells provide an antibody-to-antigen positive control for CD34 and CD45 staining in flow cytometry studies. The concentration of this cell suspension is precisely calibrated. Therefore it is also designed as an internal control for absolute count verification procedures in CD34+  HPC absolute counting process.

Compensation Setup

Cyto-Comp Cell Kit

CYTO-COMP Cell Kit is a preparation of lyophilized human lymphocytes and reconstitution buffer used in conjunction with the CYTO-COMP Reagent Kit to establish and monitor color compensation settings for multicolor analysis.
 

Cyto-Comp Reagent Kit

CYTO-COMP Reagent Kit is comprised of four sets of two-color reagents used in conjunction with the CYTO-COMP Cell Kit to establish and monitor color compensation settings for multicolor analysis.

QuickCOMP 2 Kit

The QuickCOMP 2 Kit consists of two single-color fluorescent reagents comprised of one monoclonal antibody each. Each antibody is labeled with either FITC or PE. It may be used with the CYTO-COMP Cell Kit to adjust color compensation settings on a flow cytometer equipped with AutoSetup Software.

QuickCOMP 4 Kit

The QuickCOMP 4 Kit consists of four single-color fluorescent reagents comprised of one monoclonal antibody each. Each antibody is labeled with one or four fluorochromes: FITC, PE, ECD or PC5. It may be used with the CYTO-COMP Cell Kit to adjust color compensation settings on a flow cytometer equipped with AutoSetup Software.

Stem-Comp Kit

The Stem-Comp Reagent consists of a two color fluorescent reagent implementing two monoclonal antibodies. The antibodies are conjugated to fluorescein isothiocyanate (FITC) for the fluorescence 1 channel (FL1) of a flow cytometer and R Phycoerythrin (PE) for the fluorescence 2 channel (FL2). The Stem-Comp Reagent is used to adjust color compensations for automated or manual analysis with the Stem-Kit Reagents.

VersaComp Antibody Capture Kit

VersaComp Antibody Capture Bead kit provides both positive and negative microspheres that can be used to set compensation for multicolor flow cytometry experiments. The positive population captures conjugates in single color stains and recognizes all mouse and rat isotypes, most hamster isotypes and rabbit polyclonal IgG. The negative population provides fluorescence similar to unstained cells across the different excitation/emission wavelength combinations. This combination can be used to set up compensation to correct for spectral overlap.

Cytometer Setup

APC 675-633 Set-up Kit

The APC (675/633) Setup Kit is a set of fluorospheres (fluorescent microbeads) used for daily verification of a flow cytometer’s optical alignment and fluidic stability and as an aid in standardizing the detectors on a flow cytometer for the quantitative analysis of human leukocytes. Data from each fluorosphere is collected with the 675 nm detector when excited with a 633 nm light source. The kit is comprised of two vials: one vial of Flow-Check 675 fluorospheres and one vial of Flow-Set 675 fluorospheres.

CytoFLEX Daily QC

CytoFLEX Daily QC Fluorospheres consists of a suspension of ~3 μm fluorospheres with a fluorescence emission of 410 nm to 800 nm when excited at 405 nm, 488 nm or 635 nm. CytoFLEX Daily QC Fluorospheres may be used for daily verification of the CytoFLEX flow cytometer's optical alignment and fluidics system.

Flow-Set

Flow-Set Fluorospheres are a suspension of fluorospheres (fluorescent microbeads) used as an aid in optimizing a flow cytometer for quantitative analysis of human leukocytes. Each fluorosphere contains a dye which has a fluorescent emission range of 525 nm to 700 nm when excited at 488 nm. They have a uniform size and fluorescence intensity allowing the standardization of light scatter, fluorescence intensity and optimal hydrodynamic focusing instrument settings.
 

Flow-Set Pro

Flow-Set Pro Fluorospheres is a suspension of fluorescent microspheres which may be used as an aid in standardizing a flow cytometer configured with multiple lasers (Blue, Red, and Violet). The suspension contains 3 μm (nominal diameter) polystyrene fluorospheres suspended in an aqueous medium containing surfactants and preservatives.
 

PC7 770-488 Set-up Kit

The PC7 (770/488) Setup Kit is used for daily verification of a flow cytometer’s optical alignment and fluidic stability and as an aid in standardizing the detectors on a flow cytometer for the quantitative analysis of human leukocytes. Data from each fluorosphere is collected with the 770 nm detector when excited with a 488 nm light source. The kit is comprised of two vials: one vial of Flow-Check 770 fluorospheres and one vial of Flow-Set 770 fluorospheres.

Cytometer Verification

Flow-Check Fluorospheres

Flow-Check Fluorospheres consist of 10 μm (nominal diameter) polystyrene fluorescent microspheres suspended in an aqueous medium containing surfactants and preservatives at 1 x 106 fluorospheres/mL (nominal concentration). The fluorescence emission of the dye contained within the fluorospheres ranges from 525 nm to 700 nm when excited at 488 nm. Flow-Check Fluorospheres are used to verify instrument optical alignment and fluidics.
 

Flow-Check Pro Fluorospheres

Flow-Check Pro Fluorospheres is a suspension of fluorescent microspheres which may be used for daily verification of optical alignment and fluidics of flow cytometer's configured with multiple lasers (Blue, Red, and Violet). The Fluorosphere suspension consists of a mixture of 10 μm fluorescent microbeads with a fluorescence emission of 515 nm to 800 nm when excited at 488nm, 6 μm fluorospheres with a fluorescence emission of 640 nm to 800 nm when excited at 635 nm, and 3 μm fluorospheres with a fluorescence emission of 400 nm to 500 nm when excited at 405 nm, respectively.

IMMUNO-BRITE Fluorospheres

IMMUNO-BRITE Fluorospheres are a suspension of fluorescent microbeads uniform in size with varying fluorescence intensities. The set contains one vial of non-fluorescent fluorospheres and four vials of fluorospheres of increasing intensity. They may be used to monitor instrument linearity.