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The PC7 (770/488) Setup Kit is used for daily verification of a flow cytometer’s optical alignment and fluidic stability and as an aid in standardizing the detectors on a flow cytometer for the quantitative analysis of human leukocytes. Data from each fluorosphere is collected with the 770 nm detector when excited with a 488 nm light source. The kit is comprised of two vials: one vial of Flow-Check 770 fluorospheres and one vial of Flow-Set 770 fluorospheres.