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The APC (675/633) Setup Kit is a set of fluorospheres (fluorescent microbeads) used for daily verification of a flow cytometer;s optical alignment and fluidic stability and as an aid in standardizing the detectors on a flow cytometer for the quantitative analysis of human leukocytes. Data from each fluorosphere is collected with the 675 nm detector when excited with a 633 nm light source. The kit is comprised of two vials: one vial of Flow-Check 675 fluorospheres and one vial of Flow-Set 675 fluorospheres.