The CD300a, also known as inhibitory receptor protein (IRp60), is a surface molecule of 60kDa expressed by all human natural killer (NK) cells. The IRp60 gene, located on human chromosome region 17q25 encodes a molecule, highly O- and N-glycosylated, belonging to the immunoglobulin superfamily (Ig-SF) that displays one extracellular Ig-like domain of the V-type, a hydrophobic transmembrane portion and a cytoplasmic tail containing ITIM motifs involved in the inhibitory function. The ligand of the CD300a is still unknown but CD300a does not appear to recognize HLA-class I molecules. Upon cross-linking, IRp60 recruits and activates SH2-containing phosphatases SHP-1 and SHP-2 which switch off the activating signaling cascade by dephosphorylating a variety of tyrosine phosphorylated proteins. Cross-linking of IRp60 by E59.126 monoclonal antibody strongly inhibits the spontaneous cytotoxicity of NK cells as well as the NK-mediated cytolytic activity induced via different non-HLA-specific or HLA-specific activating receptors. CD300a may play a more general role in the regulation of the immune response since it is expressed on other cell types, including T lymphocyte subsets, monocytes and granulocytes.

Specifications

Specificity CD300a
Form PE
Clone E59.126
Flow Product Line IOTest
Clone Description The E59.126 antibody has been used in flow cytometry and Western Blotting to characterize IRp60.
Isotype IgG1 Mouse
Format Liquid
Size 1 mL
Regulatory Status ASR
Target Species Human
Other Names CD300a, CMRF35H, IRp60
UNSPSC 41116015

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